CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Arylsulfatase A/ARSA was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Human Arylsulfatase A/ARSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2485) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2485-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86135-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2485-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98210-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89502-50ul | |||
![Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Western-Blot-NBP1-00154-img0006.jpg "Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00154-img0007.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-00154-img0005.jpg)
![Flow Cytometry: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Flow-Cytometry-NBP1-00154-img0008.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-00154-img0004.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-86135-img0013.jpg "Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Analysis in human testis and skeletal muscle tissues. Corresponding ARSA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-86135-img0006.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Staining of human cerebral cortex shows moderate granular cytoplasmic postivity in neurons.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-86135-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Staining of human small intestine shows moderate granular cytoplasmic postivity in glandular cells.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-86135-img0008.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Staining of human skeletal muscle shows weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-86135-img0010.jpg)
![Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-86135] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunohistochemistry-Paraffin-NBP1-86135-img0012.jpg)
![Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Anti-Arylsulfatase A/ARSA rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Western-Blot-NBP2-98210-img0002.jpg "Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Anti-Arylsulfatase A/ARSA rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Mouse Arylsulfatase A/ARSA was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-Immunoprecipitation-NBP2-98210-img0001.jpg)
![Western Blot: Arylsulfatase A/ARSA Antibody (001) [NBP2-89502] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:54 kDa Observed band size:60 kDa](http://images.novusbio.com/fullsize/Arylsulfatase-A-ARSA-Antibody-001-Western-Blot-NBP2-89502-img0001.jpg "Western Blot: Arylsulfatase A/ARSA Antibody (001) [NBP2-89502] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:54 kDa Observed band size:60 kDa")
![Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBP2-09289] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.](http://images.novusbio.com/fullsize/ARSA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09289-img0001.jpg "Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBP2-09289] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.")
![Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBL1-07736] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.](http://images.novusbio.com/fullsize/ARSA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07736-img0002.jpg "Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBL1-07736] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.")
![Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBP2-08435] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.](http://images.novusbio.com/fullsize/ARSA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08435-img0001.jpg "Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBP2-08435] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.")
![Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBP2-09290] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.](http://images.novusbio.com/fullsize/ARSA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09290-img0001.jpg "Western Blot: Arylsulfatase A/ARSA Overexpression Lysate [NBP2-09290] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSA.")
