| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-66383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42188-0.025mg | |||
NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="Mitofusin 2 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Human/Mouse/Rat Mitofusin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7884) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7884-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21710-50ul | |||
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0009.jpg "Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in PC-12 (Cat # L012V1).")
![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 5 ug/ml](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Immunohistochemistry-Paraffin-H00009927-M03-img0007.jpg)
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0004.jpg)
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0008.jpg)
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in transfected 293T cell line by MFN2 monoclonal antibody (M03), clone 4H8. Lane 1: MFN2 transfected lysatE (86.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0010.jpg)
![ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Detection limit for recombinant GST tagged MFN2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-ELISA-H00009927-M03-img0011.jpg)
![Western Blot: Mitofusin 2 Antibody (6A8) [H00009927-M01] - MFN2 monoclonal antibody (M01), clone 6A8. Analysis of MFN2 expression in PC-12.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-6A8-Western-Blot-H00009927-M01-img0007.jpg "Western Blot: Mitofusin 2 Antibody (6A8) [H00009927-M01] - MFN2 monoclonal antibody (M01), clone 6A8. Analysis of MFN2 expression in PC-12.")
![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (6A8) [H00009927-M01] - Analysis of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-6A8-Immunohistochemistry-Paraffin-H00009927-M01-img0004.jpg)
![Western Blot: Mitofusin 2 Antibody (6A8) [H00009927-M01] - MFN2 monoclonal antibody (M01), clone 6A8. Analysis of MFN2 expression in HeLa.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-6A8-Western-Blot-H00009927-M01-img0006.jpg)
![Western Blot: Mitofusin 2 Antibody (6A8) [H00009927-M01] - MFN2 monoclonal antibody (M01), clone 6A8. Analysis of MFN2 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-6A8-Western-Blot-H00009927-M01-img0008.jpg)
![ELISA: Mitofusin 2 Antibody (6A8) [H00009927-M01] - Detection limit for recombinant GST tagged MFN2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-6A8-ELISA-H00009927-M01-img0009.jpg)
![Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Western-Blot-NBP2-66383-img0003.jpg "Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody [NBP2-66383] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Mitofusin 2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-66383-img0002.jpg)
![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-66383] - Analysis of FFPE tissue section of mouse heart using Mitofusin 2/MFN2 antibody at 5 ug/mL concentration (1:200 dilution). The primary antibody binding to MFN2 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This MFN2 antibody generated a diffused to punctate cytoplasmic staining of mitochondria in the cardiac muscles (brown color). The nuclei of the muscle cells and the macrophages are stained blue in this image (40X image of a transverse section of heart tissue).](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-66383-img0001.jpg)
![Western Blot: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa Mitofusin 2 protein using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5 (NBP2-42188). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody (NBP2-42188) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-S153-5-Western-Blot-NBP2-42188-img0003.jpg "Western Blot: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa Mitofusin 2 protein using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5 (NBP2-42188). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody (NBP2-42188) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5 (NBP2-42188). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody (NBP2-42188) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Mitofusin 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-S153-5-Immunocytochemistry-Immunofluorescence-NBP2-42188-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-S153-5-Immunocytochemistry-Immunofluorescence-NBP2-42188-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5 (NBP2-42188). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody (NBP2-42188) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Mitofusin 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-S153-5-Immunocytochemistry-Immunofluorescence-NBP2-42188-img0005.jpg)
![Western Blot: Mitofusin 2 Antibody (SR2236) [NBP3-21710] - Western blot analysis using NBP3-21710 in (1) HeLa cell lysate; (2) Mouse kidney lysate.](http://images.novusbio.com/fullsize/nbp3-21710_rabbit-mitofusin-2-mab-sr2236-57202316112168.jpg "Western Blot: Mitofusin 2 Antibody (SR2236) [NBP3-21710] - Western blot analysis using NBP3-21710 in (1) HeLa cell lysate; (2) Mouse kidney lysate.")
![Immunohistochemistry: Mitofusin 2 Antibody (SR2236) [NBP3-21710] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21710.](http://images.novusbio.com/fullsize/nbp3-21710_rabbit-mitofusin-2-mab-sr2236-5720231754858.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody (SR2236) [NBP3-21710] - Immunofluorescent analysis of Hela cells, using NBP3-21710.](http://images.novusbio.com/fullsize/nbp3-21710_rabbit-mitofusin-2-mab-sr2236-57202317161891.jpg)
![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-17298] - Paraffin-embedded mouse fore brain. MFN2 antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-17298-img0003.jpg "Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-17298] - Paraffin-embedded mouse fore brain. MFN2 antibody [N1N2], N-term dilution: 1:500.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN2 antibody [N1N2], N-term (NBP2-17298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17298_rabbit-polyclonal-mitofusin-2-antibody-western-blot-261120251839621.jpg)
![Western Blot: Mitofusin 2 Antibody (4H10F8) [NBP3-15855] - Western blot analysis of extracts of various cell lines, using Mitofusin 2 antibody (NBP3-15855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H10F8-Western-Blot-NBP3-15855-img0001.jpg "Western Blot: Mitofusin 2 Antibody (4H10F8) [NBP3-15855] - Western blot analysis of extracts of various cell lines, using Mitofusin 2 antibody (NBP3-15855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (4H10F8) [Mitofusin 2] - Confocal imaging of paraffin-embedded Mouse brain tissue using Mitofusin 2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15855_rabbit-mitofusin-2-mab-4h10f8-52202517192973.jpg)
![Western Blot: Mitofusin-2 Overexpression Lysate (Adult Normal) [NBL1-13040] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mitofusin-2.](http://images.novusbio.com/fullsize/Mitofusin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13040-img0002.jpg "Western Blot: Mitofusin-2 Overexpression Lysate (Adult Normal) [NBL1-13040] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mitofusin-2.")
![Western Blot: Mitofusin-2 Overexpression Lysate (Adult Normal) [NBP2-11121] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mitofusin-2.](http://images.novusbio.com/fullsize/Mitofusin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11121-img0001.jpg "Western Blot: Mitofusin-2 Overexpression Lysate (Adult Normal) [NBP2-11121] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mitofusin-2.")
![Immunohistochemistry: Mitofusin 2 Antibody (10F6) [NBP3-15201] - IHC image of Mitofusin 2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-10F6-Immunohistochemistry-NBP3-15201-img0001.jpg "Immunohistochemistry: Mitofusin 2 Antibody (10F6) [NBP3-15201] - IHC image of Mitofusin 2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
