| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7628-SP | |||
HAF007). A specific band was detected for PUM1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, BG01V human embryonic stem cells, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PUM1 Mono-clonal Antibody (Catalog # MAB7628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PUM1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7628-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58337-25ul | |||
![Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NB100-259-img0009.jpg "Western Blot: PUM1 Antibody [NB100-259] - Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody used for WB at 0.1 ug/ml. Secondary: HRPconjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: PUM1 Antibody [NB100-259] - Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody NB100-259 used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NB100-259-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PUM1 Antibody [NB100-259] - Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody NB100-259 (lot NB100-259-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-259-2) and another rabbit anti-Pumillio 1 antibody. For blotting immunoprecipitated Pumillio 1, NB100-259 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunoprecipitation-NB100-259-img0008.jpg)
![Western Blot: PUM1 Antibody [NBP2-20011] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Western-Blot-NBP2-20011-img0002.jpg "Western Blot: PUM1 Antibody [NBP2-20011] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PUM1 Antibody [NBP2-20011] - H441 xenograft, using Pumilio 1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Immunohistochemistry-Paraffin-NBP2-20011-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP2-20011] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Pumilio-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20011-img0004.jpg)
![Pumilio 1 antibody [C2C3], C-term detects Pumilio 1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pumilio 1 stained by Pumilio 1 antibody [C2C3], C-term (NBP2-20011) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20011_rabbit-polyclonal-pum1-antibody-immunocytochemistry-immunofluorescence-2611202518441015.jpg)
![Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP1-87856] - Staining of human cell line U-251 MG shows positivity in cytoplasm.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87856-img0005.jpg "Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP1-87856] - Staining of human cell line U-251 MG shows positivity in cytoplasm.")
![<b>Independent Antibodies Validation. </b>Western Blot: PUM1 Antibody [NBP1-87856] - Analysis using Anti-PUM1 antibody NBP1-87856 (A) shows similar pattern to independent antibody NBP2-58337 (B).](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NBP1-87856-img0008.jpg)
![Immunohistochemistry-Paraffin: PUM1 Antibody [NBP1-87856] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87856-img0006.jpg)
![Western Blot: PUM1 Antibody [NBP2-58337] - Analysis using Anti-PUM1 antibody NBP2-58337 (A) shows similar pattern to independent antibody NBP1-87856 (B).](http://images.novusbio.com/fullsize/PUM1-Antibody-Western-Blot-NBP2-58337-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUM1 Antibody [NBP2-58337] - Analysis using Anti-PUM1 antibody NBP2-58337 (A) shows similar pattern to independent antibody NBP1-87856 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PUM1 Antibody [NBP2-58337] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/PUM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58337-img0001.jpg)
![Western Blot: PUM1 Antibody (8Y6H5) [NBP3-15279] - Western blot analysis of extracts of various cell lines, using PUM1 Rabbit mAb (NBP3-15279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PUM1-Antibody-8Y6H5-Western-Blot-NBP3-15279-img0002.jpg "Western Blot: PUM1 Antibody (8Y6H5) [NBP3-15279] - Western blot analysis of extracts of various cell lines, using PUM1 Rabbit mAb (NBP3-15279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: PUM1 Antibody (8Y6H5) [NBP3-15279] - Immunofluorescence analysis of U-2 OS cells using PUM1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15279_rabbit-pum1-mab-8y6h5-72202512432057.jpg)
![Western Blot: Pumilio-1 Overexpression Lysate (Adult Normal) [NBP2-10310] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pumilio-1.](http://images.novusbio.com/fullsize/Pumilio-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10310-img0001.jpg "Western Blot: Pumilio-1 Overexpression Lysate (Adult Normal) [NBP2-10310] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pumilio-1.")
