Products Related to Gene-ID: 959

F0113) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." alt="Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB1163) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

8230-CL) induces proliferation in mouse splenic B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse CD40 Ligand/TNFSF5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB1163). The ND₅₀ is typically 0.0075-0.045 µg/mL." class="big_lightbox"> 8230-CL) induces proliferation in mouse splenic B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse CD40 Ligand/TNFSF5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB1163). The ND₅₀ is typically 0.0075-0.045 µg/mL." class="big_thumb" />

MAB060)." class="big_lightbox"> MAB060)." class="big_thumb" />

MAB100) and Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB6171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD40 Ligand/TNFSF5 at approximately 15-18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> MAB100) and Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB6171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD40 Ligand/TNFSF5 at approximately 15-18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human CD4⁺cells untreated (-) or treated (+) with immobilized Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) and Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD40 Ligand/TNFSF5 Monoclonal Antibody (Catalog # MAB6171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD40 Ligand/TNFSF5 at approximately 15-18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1493-CDB) with an ED₅₀ of 2.50-30.0 ng/mL." class="big_lightbox"> 1493-CDB) with an ED₅₀ of 2.50-30.0 ng/mL." alt="Biotinylated Recombinant Human CD40 Ligand/TNFSF5 Avi-tag His-tag Protein (Catalog # AVI11596) binds Recombinant Human CD40/TNFRSF5 Fc Chimera (1493-CDB) with an ED₅₀ of 2.50-30.0 ng/mL." class="big_thumb" />

1493-CDB) with an ED₅₀ of 0.300-3.60 ng/mL." class="big_lightbox"> 1493-CDB) with an ED₅₀ of 0.300-3.60 ng/mL." alt="In a functional ELISA, Animal-Free™ Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) (Catalog # AFL617) binds to Recombinant Human CD40/TNFRSF5 Fc Chimera (1493-CDB) with an ED₅₀ of 0.300-3.60 ng/mL." class="big_thumb" />