MAB10654) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB10654) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Human Adiponectin/Acrp30 protein was serially diluted 2-fold and captured by Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog #
MAB10654) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Adiponectin/Acrp30 protein was serially diluted 2-fold and captured by Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
MAB10654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Adiponectin/Acrp30 protein was serially diluted 2-fold and captured by Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Western blot Analysis of human Kidney lysate using Adiponectin/Acrp30 Antibody (ADPN/1370).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-ADPN-1370-Western-Blot-NBP2-47652-img0004.jpg "Western Blot: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Western blot Analysis of human Kidney lysate using Adiponectin/Acrp30 Antibody (ADPN/1370).")
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Formalin-fixed, paraffin-embedded human Kidney stained with Adiponectin Monoclonal Antibody (ADPN/1370).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-ADPN-1370-Immunohistochemistry-Paraffin-NBP2-47652-img0001.jpg)
![Protein Array: Adiponectin/Acrp30 Antibody (ADPN/1370) [NBP2-47652] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Adiponectin/Acrp30 Antibody (ADPN/1370). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-ADPN-1370-Protein-Array-NBP2-47652-img0003.jpg)
![Western Blot: Adiponectin/Acrp30 Overexpression Lysate [NBL1-07342] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07342-img0002.jpg "Western Blot: Adiponectin/Acrp30 Overexpression Lysate [NBL1-07342] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adiponectin.")
![Western Blot: Adiponectin/Acrp30 Antibody (S03-9F3) [NBP3-20028] - Western blot detection of Adiponectin/Acrp30 in K562, Hela cell lysates using NBP3-20028 (1:1000 diluted). Predicted band size: 26kDa. Observed band size: 30kDa.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-S03-9F3-Western-Blot-NBP3-20028-img0001.jpg "Western Blot: Adiponectin/Acrp30 Antibody (S03-9F3) [NBP3-20028] - Western blot detection of Adiponectin/Acrp30 in K562, Hela cell lysates using NBP3-20028 (1:1000 diluted). Predicted band size: 26kDa. Observed band size: 30kDa.")
![Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.](http://images.novusbio.com/fullsize/Recombinant-Rat-Adiponectin-Acrp30-FLAG-Protein-Western-Blot-NBP3-18139-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.")
![SDS-Page: Recombinant Human Adiponectin/Acrp30 Protein [NBC1-18461] - Adiponectin, 25.1 kDa (231 aa) with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/Adiponectin-Protein-SDS-Page-NBC1-18461-img0001.jpg "SDS-Page: Recombinant Human Adiponectin/Acrp30 Protein [NBC1-18461] - Adiponectin, 25.1 kDa (231 aa) with a purity of 90% by SDS - PAGE")
![SDS-Page: Recombinant Mouse Adiponectin/Acrp30 Protein [NBC1-18447] - Adiponectin, 16 kDa (138 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Adiponectin-Protein-SDS-Page-NBC1-18447-img0001.jpg "SDS-Page: Recombinant Mouse Adiponectin/Acrp30 Protein [NBC1-18447] - Adiponectin, 16 kDa (138 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
