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![Immunohistochemistry: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32003) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32003_rabbit-adiponectin-acrp30-mab-je65-49-2982024149262.jpg "Immunohistochemistry: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32003) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Immunocytochemistry analysis of NCI-H441 cells labeling Adiponectin/Acrp30 with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32003_rabbit-adiponectin-acrp30-mab-je65-49-29202410484876.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (JE65-49) [NBP3-32003] - Western blot analysis of Adiponectin/Acrp30 on mouse colon tissue lysates with Rabbit anti-Adiponectin/Acrp30 antibody (NBP3-32003) at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 34 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32003) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32003_rabbit-adiponectin-acrp30-mab-je65-49-392024848505.jpg)
![Western Blot: Adiponectin/Acrp30 Overexpression Lysate [NBL1-07342] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07342-img0002.jpg "Western Blot: Adiponectin/Acrp30 Overexpression Lysate [NBL1-07342] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adiponectin.")
![Western Blot: Adiponectin/Acrp30 Antibody (S03-9F3) [NBP3-20028] - Western blot detection of Adiponectin/Acrp30 in K562, Hela cell lysates using NBP3-20028 (1:1000 diluted). Predicted band size: 26kDa. Observed band size: 30kDa.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-S03-9F3-Western-Blot-NBP3-20028-img0001.jpg "Western Blot: Adiponectin/Acrp30 Antibody (S03-9F3) [NBP3-20028] - Western blot detection of Adiponectin/Acrp30 in K562, Hela cell lysates using NBP3-20028 (1:1000 diluted). Predicted band size: 26kDa. Observed band size: 30kDa.")
![Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.](http://images.novusbio.com/fullsize/Recombinant-Rat-Adiponectin-Acrp30-FLAG-Protein-Western-Blot-NBP3-18139-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.")
![SDS-Page: Recombinant Human Adiponectin/Acrp30 Protein [NBC1-18461] - Adiponectin, 25.1 kDa (231 aa) with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/Adiponectin-Protein-SDS-Page-NBC1-18461-img0001.jpg "SDS-Page: Recombinant Human Adiponectin/Acrp30 Protein [NBC1-18461] - Adiponectin, 25.1 kDa (231 aa) with a purity of 90% by SDS - PAGE")
![SDS-Page: Recombinant Mouse Adiponectin/Acrp30 Protein [NBC1-18447] - Adiponectin, 16 kDa (138 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Adiponectin-Protein-SDS-Page-NBC1-18447-img0001.jpg "SDS-Page: Recombinant Mouse Adiponectin/Acrp30 Protein [NBC1-18447] - Adiponectin, 16 kDa (138 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![Goat Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77003] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-77003_goat-adiponectin-acrp30-elisa-kit-colorimetric-3120248343432.png "Goat Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77003] - Standard Curve Reference")
![Monkey Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77001] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-77001_monkey-adiponectin-acrp30-elisa-kit-colorimetric-3120248485422.png "Monkey Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77001] - Standard Curve Reference")
![Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77004] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-77004_sheep-adiponectin-acrp30-elisa-kit-colorimetric-3120248485446.png "Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77004] - Standard Curve Reference")