TRAILR2/TNFRSF10B: Novus Biologicals

NB100-56618

TRAILR2/TNFRSF10B Antibody - BSA Free

(1 Review)

(43 Publications)

0.1 mg

NB100-56618

<b>Biological Strategies Validation. </b>TRAILR2-TNFRSF10B-Antibody-Immunohistochemistry-NB100-56618-img0008.jpg

Immunocytochemistry/Immunofluorescence: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TRAIL R2 (H76) at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis using the Azide Free version of NB100-56618. Detection of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL-R2 at 5 ug/ml.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-120A

Alt Catalog# IMG-120A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB100-55744
BSA Free	NB100-56618SS

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AF631

TRAILR2/TNFRSF10B Antibody [Unconjugated]

(32 Publications)

100 μg

AF631

HAF019). A specific band was detected for TRAIL R2/TNFRSF10B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF631) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). A specific band was detected for TRAIL R2/TNFRSF10B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF631) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for TRAIL R2/TNFRSF10B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

UL141 Blockade of Both TRAIL DR and CD155 Contributes to NK Cell Inhibition(A) RAd control or UL141 transduced A549 cells were analyzed for expression of TRAIL-R2, CD155 and CD112 at the time of NK cell addition by flow cytometry.(B) Western blot of RAd-transduced A549 cells.(C) Expression of TRAIL by IFN alpha activated (blue) or unactivated (red) human NK cells assessed by flow cytometry.(D) IFN alpha -activated NK cells were purified from human peripheral blood and added to A549 lung epithelial cells transduced with either control adenovirus vector (Rad-Cntrl) or Rad-UL141 (effector to target [E:T] ratio of 2). Blocking alpha DNAM-1 antibody or blocking soluble TRAIL-R2 (10 μg/ml) was added to cultures where indicated (+), and control mIgG or sCD30 was added as a control to the other cultures. Apoptosis of A549 cells was assessed 4 hr later. Shown are two representative experiments of more than six performed.(E) Summary of NK killing data from eight separate experiments and four different donors. †Data are mean ± SEM from n = 1 to 3 wells. ∗Bonferroni post test shows significance at p < 0.05.See also Figure S5. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23498957), licensed under a CC-BY license. Not internally tested by R&D Systems.

TRAIL-R2 Expression in HCMV-Infected Cells Human foreskin fibroblasts (HFFs) were infected (72 hr, MOI = 20) with HCMV Merlin (Mer) or Merlin delta UL141 (Mer delta 141) and analyzed for TRAIL-R2 expression by (A) flow cytometry and (B) Western blot. IgG(–), isotype control antibody staining. Results are representative of six to ten performed experiments. See also Figure S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23498957), licensed under a CC-BY license. Not internally tested by R&D Systems.

PERK pathway activation upon ER stress in bidimensional cultures and multicellular tumor spheroids.HCT116 cells, in conventional 2D cultures or spheroids (3D) (10-days), were treated with TG (100 nM) for the indicated times. Activation of the PERK signaling pathway (A) and TRAIL-R2/DR5 protein levels (B) were assessed in whole-cell extracts by western blotting. alpha -tubulin or GAPDH were used as protein-loading controls. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35115486), licensed under a CC-BY license. Not internally tested by R&D Systems.

cFLIP levels and caspase-8 activation upon ER stress in tumor cells.HCT116 cells were treated with TG (100 nM) for the indicated times. cFLIP and CHOP levels (A) as well as TRAIL-R2/DR5 upregulation, caspase-8 and caspase-3 processing (B) were determined in whole-cell extracts by western blotting. Levels of both cFLIP isoforms were quantified with Image LabTM 6.0 software using GAPDH as protein-loading control and graphed relative to time 0 levels. Blots are representative of three independent experiments. In A, two different exposures of the western blot are shown to follow the levels of the short isoform of cFLIP. C HCT116 cells were treated with TG (100 nM) for the indicated times and mRNA relative levels of cFLIPL (upper panel) and cFLIPS (lower panel) were examined by RT-qPCR as described in materials and methods and referred to time 0 h levels (ns = not statistically significant. Unpaired t test with Welch’s correction). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35115486), licensed under a CC-BY license. Not internally tested by R&D Systems.

GCN2-dependent activation of the extrinsic pathway of apoptosis in tumor cells treated with AOA.A Apoptosis in HCT116 cells cultured for 48 h in complete medium with or without AOA in the presence or absence of non-essential amino acids (NEAA). B, C HCT116 cells were transfected either with a scrambled oligonucleotide (Sc) or with two different siRNAs targeting GCN2 (siGCN2#1 or #2). 48 hours after transfection, cells were either incubated for 16 h in the presence of absence of AOA to assess phosphorylated eIF2 alpha , eIF2 alpha and CHOP levels by Western blotting (B), or during 24 h to measure apoptosis (C). D HCT116 cells were transfected and treated as described in B. TRAIL-R2 and GCN2 levels were assessed by Western blotting. GAPDH was used as protein-loading control. E HCT116 cells stably expressing scrambled, caspase-8, or TRAIL-R2 targeting shRNA, were cultured in the presence or absence of AOA, and apoptosis was measured at 24 or 48 h. Caspase 8 and TRAIL-R2 knockdown were determined by Western blotting. In A, C and E, data are presented as mean ± SD from at least three independent experiments. *P < 0.05; ***P < 0.001; ****P < 0.0001; two-way ANOVA test. Tukey’s multiple comparison test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36302756), licensed under a CC-BY license. Not internally tested by R&D Systems.

Role of FLIP in cell death induced by glutamine deprivation.A HCT116 (left panel) or MDA-MB468 (right panel) cells were cultured in the presence or absence of glutamine for the indicated times. Tubulin and GAPDH were used as protein-loading controls. Following these treatments, FLIPL and FLIPS levels were assessed by Western blotting. B Apoptosis was assessed in pBabe or FLIPL overexpressing HCT116 cells cultured in the presence or absence of glutamine for 48 h (left panel). Procaspase-8 levels, caspase-8 activation (cC8) and FLIPL overexpression were determined by Western-blotting after 30 h of glutamine starvation (right panel). C Apoptosis was assessed in pBabe or FLIPL-overexpressing MDA-MB468 cells cultured in the presence or absence of glutamine for 48 h. FLIPL overexpression was detected by Western blotting. In B and C data are presented as mean ± SD from at least three independent experiments. **P < 0.01; ***P < 0.001; two-way ANOVA test. Tukey’s multiple comparison test. D HCT116 cells were cultured in the presence or absence of glutamine for 16 h, with or without dimethyl alpha -ketoglutarate (5 mM). FLIP levels, ISR activation and TRAIL-R2 upregulation were assessed by Western blotting. In E apoptosis was assessed in HCT116 cells cultured in the presence or absence of glutamine for 48 hours, with or without dimethyl alpha -ketoglutarate. Data are presented as mean ± SD from at least three independent experiments. **P < 0.01; ***P < 0.001; two-way ANOVA test. Tukey’s multiple comparison test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36302756), licensed under a CC-BY license. Not internally tested by R&D Systems.

Role of TRAIL-R2 in apoptosis induced by glutamine deprivation in tumor cells.A (HCT116) or B (MDA-MB468) cells stably expressing a scrambled (shSc) or a TRAIL-R2 targeting shRNA (shTRAIL-R2#1) were cultured with or without glutamine and apoptosis was assessed at the indicated times (HCT116) or 48 h (MDA-MB468). TRAIL-R2 knockdown was determined by Western blotting. Tubulin and GAPDH were used as protein-loading controls. Data are presented as mean ± SD from at least three independent experiments. **P < 0.01; ****P < 0.0001; two-way ANOVA test. Tukey’s multiple comparison test. C Procaspase-8 levels and caspase-8 activation (cC8) in HCT116 cells incubated in the presence or absence of glutamine for the indicated times. D Scrambled (Sc) or shTRAIL-R2#1 HCT116 cells were cultured in the presence or absence of glutamine for 24 h. Following this incubation, TRAIL-R2 levels, caspase-8 and caspase-3 activation, as well as procaspase-8 or procaspase-3 levels were assessed by Western blotting. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36302756), licensed under a CC-BY license. Not internally tested by R&D Systems.

GCN2-dependent TRAIL-R2 upregulation upon glutamine deprivation in tumor cells.A HCT116 cells were cultured in the presence or absence of glutamine for the indicated times and TRAIL-R2 mRNA levels were measured by RT-qPCR (left panel). TRAIL-R2 and TRAIL-R1 protein levels were assessed by Western blotting (middle panel). GAPDH was used as protein-loading control. Cell surface TRAIL-R2 levels (right panel) were also analyzed in HCT116 and MDA-MB468 cell lines after 24 h of glutamine deprivation in the presence of 20 µM Q-VD-OPh, as described in Materials and Methods (MFI: geometric mean fluorescent intensity). B HCT116 cells were incubated for the indicated times in medium with or without glutamine, in the presence or absence of 1 µM A92. Western blotting was performed to determine TRAIL-R2, p-eIF2 alpha , eIF2 alpha and CHOP levels. C HCT116 cells stably expressing either a scrambled (Sc) or a GCN2 targeting sequence (shGCN2), were incubated for 17 h in the presence or absence of glutamine. Following this incubation, TRAIL-R2 mRNA levels were measured by RT-qPCR (left panel) and GCN2, CHOP and TRAIL-R2 protein levels were assessed by Western blotting (right panel). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36302756), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF631U-100UG
Alexa Fluor 405	AF631V-100UG
Alexa Fluor 488	AF631G-100UG
Alexa Fluor 532	AF631X-100UG
Alexa Fluor 594	AF631T-100UG
Alexa Fluor 647	AF631R-100UG
Alexa Fluor 700	AF631N-100UG
Alexa Fluor 750	AF631S-100UG
Alexa Fluor Plus 405	AF631AFP405-100UG
Alexa Fluor Plus 488	AF631AFP488-100UG
Alexa Fluor Plus 555	AF631AFP555-100UG
Alexa Fluor Plus 594	AF631AFP594-100UG
Alexa Fluor Plus 647	AF631AFP647-100UG
Alexa Fluor Plus 680	AF631AFP680-100UG
Allophycocyanin	AF631A
Biotin	BAF631
CoraFluor 1	AF631CL1
DyLight 350	AF631D
DyLight 405	AF631E
DyLight 488	AF631K
DyLight 550	AF631L
DyLight 594	AF631M
DyLight 650	AF631W
DyLight 680	AF631Y
DyLight 755	AF631Z
FITC	AF631F
HRP	AF631H
Janelia Fluor 525	AF631JF525
Janelia Fluor 549	AF631I
Janelia Fluor 646	AF631J
Janelia Fluor 669	AF631JF669
PE	AF631P
PerCP	AF631C
mFluor Violet 450 SE	AF631MFV450
mFluor Violet 500 SE	AF631MFV500
mFluor Violet 610 SE	AF631MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF631-SP

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MAB6311

TRAILR2/TNFRSF10B Antibody (71908) [Unconjugated]

(25 Publications)

500 μg

MAB6311

Flow cytometry studies of scFv-Fc-scTRAIL molecules and Fc-scTRAIL on Colo205 and HCT116 cells(A) Expression levels of EGFR, HER2, HER3, EpCAM, and TRAIL-receptors were investigated after treatment of the cells with medium or 250 ng/ml BZB for 16 h. (B) Serial dilutions of the molecules were analyzed. (C) 20 nM scTRAIL units were analyzed after preincubation of the cells with PBA or a 200-fold molar excess of the respective blocking antibody. Binding of Fc-scTRAIL was measured in the presence of all blocking antibodies separately and is here represented as the mean of blocking with all different antibodies. Bound molecules were detected via anti-FLAG-PE. relative MFI, relative median fluorescence intensity. Pairwise comparisons were performed by unpaired t test (two-tailed; *P 0.05). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.24379), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6311U-100UG
Alexa Fluor 405	FAB6311V-100UG
Alexa Fluor 488	FAB6311G-025
Alexa Fluor 488	FAB6311G-100
Alexa Fluor 532	FAB6311X
Alexa Fluor 594	FAB6311T-100UG
Alexa Fluor 700	FAB6311N-100
Alexa Fluor 700	FAB6311N-025
Alexa Fluor 750	FAB6311S-100UG
Alexa Fluor Plus 405	FAB6311AFP405-100UG
Alexa Fluor Plus 488	FAB6311AFP488-100UG
Alexa Fluor Plus 555	FAB6311AFP555-100UG
Alexa Fluor Plus 594	FAB6311AFP594-100UG
Alexa Fluor Plus 647	FAB6311AFP647-100UG
Alexa Fluor Plus 680	FAB6311AFP680-100UG
Allophycocyanin	FAB6311A-100
Allophycocyanin	FAB6311A-025
Allophycocyanin/Cy7	FAB6311APCCY7
Biotin	FAB6311B
CoraFluor 1	FAB6311CL1
DyLight 350	FAB6311D
DyLight 405	FAB6311E
DyLight 488	FAB6311K
DyLight 550	FAB6311L
DyLight 594	FAB6311M
DyLight 650	FAB6311W
DyLight 680	FAB6311Y
DyLight 755	FAB6311Z
FITC	FAB6311F
HRP	FAB6311H
Janelia Fluor 525	FAB6311JF525
Janelia Fluor 549	FAB6311I
Janelia Fluor 646	FAB6311J
Janelia Fluor 669	FAB6311JF669
PE/Atto594	FAB6311PEATT594
PE/Cy5.5	FAB6311PECY55
PE/Cy7	FAB6311PECY7
Phycoerythrin	FAB6311P-100
Phycoerythrin	FAB6311P-025
mFluor Violet 450 SE	FAB6311MFV450
mFluor Violet 500 SE	FAB6311MFV500
mFluor Violet 610 SE	FAB6311MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6311-SP
Carrier Free	FAB6311N-100
Carrier Free	FAB6311N-025
Carrier Free	FAB6311A-100
Carrier Free	FAB6311A-025
Carrier Free	FAB6311G-100
Carrier Free	FAB6311G-025
Carrier Free	FAB6311P-100
Carrier Free	FAB6311P-025
Carrier Free	FAB6311V-100UG
Carrier Free	FAB6311U-100UG
Carrier Free	FAB6311T-100UG
Carrier Free	FAB6311S-100UG

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MAB631

TRAILR2/TNFRSF10B Antibody (71903) [Unconjugated]

(19 Publications)

100 μg

MAB631

Human TRAIL R2/TNFRSF10B Monoclonal Antibody induces apoptosis of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner, as measured by Resazurin (Catalog # AR002) . The ED50 for this effect is typically 2-12 ng/mL.

<b>Biological Strategies Validation. </b>Cleavage of FRET probe is indicative of caspase activation.Cells were treated with (A) 0–300 ng/ml TRAIL for 2.5 hours or (B) 0–50 µg/ml anti-DR5 antibody for 20 h. The resultant cells were analyzed by western blotting using antibodies specific to caspase 3 (C3), caspase 8 (C8), and CFP/YFP. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0107010), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>MB231_CFP-YFP cells retain sensitivity to TRAIL and anti-DR5 antibody.(A) Parental MB231 (filled black) and MB231_CFP-YFP (open clear) cells were treated with TRAIL (0–100 ng/ml) for 2.5 h or anti-DR5 antibody (0–200 µg/ml) for 20 h, at the indicated concentrations. Cell viability was determined by MTT assay. (B) Cells were treated as in A and analyzed for apoptosis by flow cytometry after staining with Annexin-V-APC and propidium iodide (PI). p-values were determined using a Student’s t-test. EC50 values were determined using nonlinear curve fitting as described in the Materials and Methods section. For each EC50 value, 95% confidence intervals reflecting the statistical accuracy are reported in Table 1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0107010), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Cell-based FRET biosensor provides a quantifiable response to treatments of death receptor targeted cancer therapies.MB231_CFP-YFP cells were treated with (A) 0–300 ng/ml TRAIL for 2.5 h and (B) 0–100 µg/ml anti-DR5 antibody for 20 h. FRET was calculated and is represented using bar graphs on the left side of the panel. EC50 values were determined by nonlinear curve fitting to normalized FRET values plotted against the log values of TRAIL and anti-DR5 antibody concentration in molar (right side of the panel). (C) Confocal microscopy images showing changes in FRET in MB231_CFP-YFP cells treated with 0–25 ng/ml TRAIL for 2.5 h. A pronounced shift from YFP acceptor emission (yellow) to CFP donor emission (cyan) can be observed for cells treated with increasing concentration of TRAIL, with an excitation setting = 458 nm. Statistical analysis was performed as in Fig. 1 and 2. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0107010), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB631U-100UG
Alexa Fluor 405	FAB631V-100UG
Alexa Fluor 488	FAB631G-100UG
Alexa Fluor 532	FAB631X-100UG
Alexa Fluor 594	FAB631T-100UG
Alexa Fluor 647	FAB631R-100UG
Alexa Fluor 700	FAB631N-100UG
Alexa Fluor 750	FAB631S-100UG
Alexa Fluor Plus 405	FAB631AFP405-100UG
Alexa Fluor Plus 488	FAB631AFP488-100UG
Alexa Fluor Plus 555	FAB631AFP555-100UG
Alexa Fluor Plus 594	FAB631AFP594-100UG
Alexa Fluor Plus 647	FAB631AFP647-100UG
Alexa Fluor Plus 680	FAB631AFP680-100UG
Allophycocyanin	FAB631A
Biotin	FAB631B
CoraFluor 1	FAB631CL1
DyLight 350	FAB631D
DyLight 405	FAB631E
DyLight 488	FAB631K
DyLight 550	FAB631L
DyLight 594	FAB631M
DyLight 650	FAB631W
DyLight 680	FAB631Y
DyLight 755	FAB631Z
FITC	FAB631F
HRP	FAB631H
Janelia Fluor 525	FAB631JF525
Janelia Fluor 549	FAB631I
Janelia Fluor 646	FAB631J
Janelia Fluor 669	FAB631JF669
PE	FAB631P
PerCP	FAB631C
mFluor Violet 450 SE	FAB631MFV450
mFluor Violet 500 SE	FAB631MFV500
mFluor Violet 610 SE	FAB631MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB631-SP
Carrier Free	MAB631-500

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AF721

TRAILR2/TNFRSF10B Antibody [Unconjugated]

(5 Publications)

100 μg

AF721

MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF721). The ND₅₀ is typically 0.4-1.2 µg/mL." class="big_lightbox">

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # <a class=

MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF721). The ND₅₀ is typically 0.4-1.2 µg/mL." alt="In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF721). The ND₅₀ is typically 0.4-1.2 µg/mL." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF721U-100UG
Alexa Fluor 405	AF721V-100UG
Alexa Fluor 488	AF721G-100UG
Alexa Fluor 532	AF721X-100UG
Alexa Fluor 594	AF721T-100UG
Alexa Fluor 647	AF721R-100UG
Alexa Fluor 700	AF721N-100UG
Alexa Fluor 750	AF721S-100UG
Alexa Fluor Plus 405	AF721AFP405-100UG
Alexa Fluor Plus 488	AF721AFP488-100UG
Alexa Fluor Plus 555	AF721AFP555-100UG
Alexa Fluor Plus 594	AF721AFP594-100UG
Alexa Fluor Plus 647	AF721AFP647-100UG
Alexa Fluor Plus 680	AF721AFP680-100UG
Biotin	BAF721
CoraFluor 1	AF721CL1
DyLight 350	AF721D
DyLight 405	AF721E
DyLight 488	AF721K
DyLight 550	AF721L
DyLight 594	AF721M
DyLight 650	AF721W
DyLight 680	AF721Y
DyLight 755	AF721Z
FITC	AF721F
HRP	AF721H
Janelia Fluor 525	AF721JF525
Janelia Fluor 549	AF721I
Janelia Fluor 646	AF721J
Janelia Fluor 669	AF721JF669
PE	AF721P
mFluor Violet 450 SE	AF721MFV450
mFluor Violet 500 SE	AF721MFV500
mFluor Violet 610 SE	AF721MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF721-SP

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NBP2-46148

TRAILR2/TNFRSF10B Antibody (OTI2A5)

0.1 ml

NBP2-46148

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74578AF350
Alexa Fluor 405	NBP2-74578AF405
Alexa Fluor 488	NBP2-74578AF488
Alexa Fluor 532	NBP2-74578AF532
Alexa Fluor 594	NBP2-74578AF594
Alexa Fluor 647	NBP2-74578AF647
Alexa Fluor 700	NBP2-74578AF700
Alexa Fluor 750	NBP2-74578AF750
Alexa Fluor Plus 405	NBP2-74578AFP405
Alexa Fluor Plus 488	NBP2-74578AFP488
Alexa Fluor Plus 555	NBP2-74578AFP555
Alexa Fluor Plus 594	NBP2-74578AFP594
Alexa Fluor Plus 647	NBP2-74578AFP647
Alexa Fluor Plus 680	NBP2-74578AFP680
Allophycocyanin	NBP2-74578APC
Biotin	NBP2-74578B
CoraFluor 1	NBP2-74578CL1
DyLight 350	NBP2-74578UV
DyLight 405	NBP2-74578V
DyLight 488	NBP2-74578G
DyLight 550	NBP2-74578R
DyLight 594	NBP2-74578DL594
DyLight 650	NBP2-74578C
DyLight 680	NBP2-74578FR
DyLight 755	NBP2-74578IR
FITC	NBP2-74578F
HRP	NBP2-74578H
Janelia Fluor 525	NBP2-74578JF525
Janelia Fluor 549	NBP2-74578JF549
Janelia Fluor 646	NBP2-74578JF646
Janelia Fluor 669	NBP2-74578JF669
PE	NBP2-74578PE
PerCP	NBP2-74578PCP
mFluor Violet 450 SE	NBP2-74578MFV450
mFluor Violet 500 SE	NBP2-74578MFV500
mFluor Violet 610 SE	NBP2-74578MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74578

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MAB1540

TRAILR2/TNFRSF10B Antibody (118929) [Unconjugated]

(2 Publications)

500 μg

MAB1540

CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of Purkinje neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

TRAIL R2/TNFRSF10B was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Rat Anti-Mouse TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB1540) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of Purkinje neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="TRAIL R2/TNFRSF10B was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Rat Anti-Mouse TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB1540) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of Purkinje neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1540U-100UG
Alexa Fluor 405	FAB1540V-100UG
Alexa Fluor 488	FAB1540G-100UG
Alexa Fluor 532	FAB1540X-100UG
Alexa Fluor 594	FAB1540T-100UG
Alexa Fluor 647	FAB1540R-100UG
Alexa Fluor 700	FAB1540N-100UG
Alexa Fluor 750	FAB1540S-100UG
Alexa Fluor Plus 405	FAB1540AFP405-100UG
Alexa Fluor Plus 488	FAB1540AFP488-100UG
Alexa Fluor Plus 555	FAB1540AFP555-100UG
Alexa Fluor Plus 594	FAB1540AFP594-100UG
Alexa Fluor Plus 647	FAB1540AFP647-100UG
Alexa Fluor Plus 680	FAB1540AFP680-100UG
Allophycocyanin	FAB1540A
Biotin	FAB1540B
CoraFluor 1	FAB1540CL1
DyLight 350	FAB1540D
DyLight 405	FAB1540E
DyLight 488	FAB1540K
DyLight 550	FAB1540L
DyLight 594	FAB1540M
DyLight 650	FAB1540W
DyLight 680	FAB1540Y
DyLight 755	FAB1540Z
FITC	FAB1540F
HRP	FAB1540H
Janelia Fluor 525	FAB1540JF525
Janelia Fluor 549	FAB1540I
Janelia Fluor 646	FAB1540J
Janelia Fluor 669	FAB1540JF669
PE	FAB1540P
PerCP	FAB1540C
mFluor Violet 450 SE	FAB1540MFV450
mFluor Violet 500 SE	FAB1540MFV500
mFluor Violet 610 SE	FAB1540MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1540-SP

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10140-T2

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

(3 Publications)

100 μg

10140-T2

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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721-DR

Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

(2 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB6313

TRAILR2/TNFRSF10B Antibody (603307)

100 μg

MAB6313

HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6313U-100UG
Alexa Fluor 405	FAB6313V-100UG
Alexa Fluor 488	FAB6313G-100UG
Alexa Fluor 532	FAB6313X-100UG
Alexa Fluor 594	FAB6313T-100UG
Alexa Fluor 647	FAB6313R-100UG
Alexa Fluor 700	FAB6313N-100UG
Alexa Fluor 750	FAB6313S-100UG
Alexa Fluor Plus 405	FAB6313AFP405-100UG
Alexa Fluor Plus 488	FAB6313AFP488-100UG
Alexa Fluor Plus 555	FAB6313AFP555-100UG
Alexa Fluor Plus 594	FAB6313AFP594-100UG
Alexa Fluor Plus 647	FAB6313AFP647-100UG
Alexa Fluor Plus 680	FAB6313AFP680-100UG
Allophycocyanin	FAB6313A
Biotin	FAB6313B
CoraFluor 1	FAB6313CL1
DyLight 350	FAB6313D
DyLight 405	FAB6313E
DyLight 488	FAB6313K
DyLight 550	FAB6313L
DyLight 594	FAB6313M
DyLight 650	FAB6313W
DyLight 680	FAB6313Y
DyLight 755	FAB6313Z
FITC	FAB6313F
HRP	FAB6313H
Janelia Fluor 525	FAB6313JF525
Janelia Fluor 549	FAB6313I
Janelia Fluor 646	FAB6313J
Janelia Fluor 669	FAB6313JF669
PE	FAB6313P
PerCP	FAB6313C
mFluor Violet 450 SE	FAB6313MFV450
mFluor Violet 500 SE	FAB6313MFV500
mFluor Violet 610 SE	FAB6313MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6313-SP

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AFR10140

Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

20 μg

AFR10140

Streptavidin coated beads conjugated to biotinylated anti-human TRAILR2 Antibody were stained with the indicated concentrations of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 647 (Catalog # AFR10140).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AFG10140

Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein

20 μg

AFG10140

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 8795

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TRAILR2/TNFRSF10B Antibody (71908) [Unconjugated]

TRAILR2/TNFRSF10B Antibody (71903) [Unconjugated]

TRAILR2/TNFRSF10B Antibody [Unconjugated]

TRAILR2/TNFRSF10B Antibody (OTI2A5)

TRAILR2/TNFRSF10B Antibody (118929) [Unconjugated]

Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

TRAILR2/TNFRSF10B Antibody (603307)

Recombinant Human TRAIL R2 Fc Alexa Fluor® 647 Protein

Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein