MAB633R) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_lightbox">

MAB633R) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." alt="Recombinant Human TRAILR4/TNFRSF10D protein was serially diluted 2-fold and captured by Mouse Anti-Human TRAILR4/TNFRSF10D Monoclonal Antibody (
MAB633R) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_thumb" />
MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog #
633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog #
375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R4/ TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633). The ND
50 is typically 0.03-0.1 µg/mL." class="big_lightbox">

MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog #
633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog #
375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R4/ TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633). The ND
50 is typically 0.03-0.1 µg/mL." class="big_thumb" />