MAB758) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB758) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Mouse Chordin protein was serially diluted 2-fold and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog #
MAB758) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB7582) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB7582) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Chordin was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse Chordin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF758) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
758-CN) was serially diluted and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB758) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Chordin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF758) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse Chordin (Catalog # 758-CN) was serially diluted and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB758) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Chordin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF758) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
MAB758) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Chordin protein was serially diluted 2-fold and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB758) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Chordin protein was serially diluted 2-fold and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # MAB7581) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
758-CN) was serially diluted and captured by Rat Anti-Mouse Chordin Monoclonal Antibody (Catalog # 
MAB7582) coated on a Clear Polystyrene Microplate (Catalog # ![Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-98689] - Staining of human Chordin in human hepatoma with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Chordin-Antibody-Immunohistochemistry-Paraffin-NBP2-98689-img0002.jpg "Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-98689] - Staining of human Chordin in human hepatoma with rabbit polyclonal antibody (1:1000).")
![Immunocytochemistry/Immunofluorescence: Chordin Antibody [NBP2-98689] - Immunofluorescence staining of Chordin in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98689 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(TM)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98689_rabbit-polyclonal-chordin-antibody-235202521525073.png)
![Immunohistochemistry-Paraffin: Chordin Antibody (065) [NBP2-89221] - Staining of human CHRD in human hepatoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Chordin-Antibody-065-Immunohistochemistry-Paraffin-NBP2-89221-img0002.jpg "Immunohistochemistry-Paraffin: Chordin Antibody (065) [NBP2-89221] - Staining of human CHRD in human hepatoma with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Chordin Antibody (065) [NBP2-89221] - Staining of human CHRD in human liver with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Chordin-Antibody-065-Immunohistochemistry-Paraffin-NBP2-89221-img0001.jpg)
![Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-99454] - Immunochemical staining of human Chordin in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Chordin-Antibody-Immunohistochemistry-Paraffin-NBP2-99454-img0002.jpg "Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-99454] - Immunochemical staining of human Chordin in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-99454] - Immunochemical staining of human Chordin in human hepatoma with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Chordin-Antibody-Immunohistochemistry-Paraffin-NBP2-99454-img0001.jpg)
![Immunohistochemistry-Paraffin: Chordin Antibody [NBP1-87513] - Staining of human duodenum shows strong plasma positivity.](http://images.novusbio.com/fullsize/Chordin-Antibody-Immunohistochemistry-Paraffin-NBP1-87513-img0002.jpg "Immunohistochemistry-Paraffin: Chordin Antibody [NBP1-87513] - Staining of human duodenum shows strong plasma positivity.")
![Western Blot: Chordin Overexpression Lysate (Adult Normal) [NBP2-10545] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chordin.](http://images.novusbio.com/fullsize/Chordin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10545-img0001.jpg "Western Blot: Chordin Overexpression Lysate (Adult Normal) [NBP2-10545] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chordin.")
![Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-98688] - Immunochemical staining of human Chordin in human liver (from 2 donors) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Chordin-Antibody-Immunohistochemistry-Paraffin-NBP2-98688-img0001.jpg "Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-98688] - Immunochemical staining of human Chordin in human liver (from 2 donors) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
