| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47524-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8098-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20135-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22125-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26694-50ul | |||
![Immunocytochemistry/Immunofluorescence: Cbx2 Antibody [NBP2-47524] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47524-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cbx2 Antibody [NBP2-47524] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Cbx2 Antibody [NBP2-47524] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Immunohistochemistry-Paraffin-NBP2-47524-img0008.jpg)
![Immunohistochemistry-Paraffin: Cbx2 Antibody [NBP2-47524] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Immunohistochemistry-Paraffin-NBP2-47524-img0005.jpg)
![Immunohistochemistry-Paraffin: Cbx2 Antibody [NBP2-47524] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Immunohistochemistry-Paraffin-NBP2-47524-img0006.jpg)
![Immunohistochemistry-Paraffin: Cbx2 Antibody [NBP2-47524] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Immunohistochemistry-Paraffin-NBP2-47524-img0007.jpg)
![Flow Cytometry: Cbx2 Antibody (RAB-C238) - Chimeric - Azide and BSA Free [NBP3-20135] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C238 (NBP3-20135, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20135_rabbit-cbx2-mab-rab-c238-chimeric-azide-and-bsa-free-2742023924270.png "Flow Cytometry: Cbx2 Antibody (RAB-C238) - Chimeric - Azide and BSA Free [NBP3-20135] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C238 (NBP3-20135, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Cbx2 Antibody (RAB-C238) - Chimeric - Azide and BSA Free [NBP3-20135] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C238 (NBP3-20135) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20135, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20135_rabbit-cbx2-mab-rab-c238-chimeric-azide-and-bsa-free-2742023924222.png)
![Western Blot: CBX2 Antibody [NBP2-15735] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CBX2-Antibody-Western-Blot-NBP2-15735-img0001.jpg "Western Blot: CBX2 Antibody [NBP2-15735] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CBX2 Antibody [NBP2-15735] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CBX2-Antibody-Immunohistochemistry-Paraffin-NBP2-15735-img0002.jpg)
![Western Blot: Cbx2 Antibody (JE34-45) [NBP3-32114] - Western blot analysis of Cbx2 on different lysates with Rabbit anti-Cbx2 antibody (NBP3-32114) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: Mouse heart tissue lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32114_rabbit-cbx2-mab-je34-45-27820241524261.jpg "Western Blot: Cbx2 Antibody (JE34-45) [NBP3-32114] - Western blot analysis of Cbx2 on different lysates with Rabbit anti-Cbx2 antibody (NBP3-32114) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: Mouse heart tissue lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Cbx2 Antibody (JE34-45) [NBP3-32114] - Flow cytometric analysis of HeLa cells labeling Cbx2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32114, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32114_rabbit-cbx2-mab-je34-45-29202416372149.jpg)
![Chromatin Immunoprecipitation Sequencing: Cbx2 Antibody [NBP1-40342] - Localization of CBX2 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX2 antibody NBP1-40342 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX2 binding within a 1.5 Mb region of chromosome 1 as detected using anti-CBX2 antibody NBP1-40342. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP1-40342-img0004.jpg "Chromatin Immunoprecipitation Sequencing: Cbx2 Antibody [NBP1-40342] - Localization of CBX2 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX2 antibody NBP1-40342 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX2 binding within a 1.5 Mb region of chromosome 1 as detected using anti-CBX2 antibody NBP1-40342. ChIP-seq validation performed by Diogenode, Denville, NJ.")
![Immunoprecipitation: Cbx2 Antibody [NBP1-40342] - Detection of human CBX2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX2 antibody NBP1-40342 (lot NBP1-40342-3) used for IP at 6 ug per reaction. CBX2 was also immunoprecipitated by previous lots of this antibody (lots NBP1-40342-1 and NBP1-40342-2). For blotting immunoprecipitated CBX2, NBP1-40342 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cbx2-Antibody-Immunoprecipitation-NBP1-40342-img0005.jpg)
![Western Blot: Cbx2 Antibody (3Z6W6) [NBP3-16164] - Western blot analysis of extracts of various cell lines, using Cbx2 Rabbit mAb (NBP3-16164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cbx2-Antibody-3Z6W6-Western-Blot-NBP3-16164-img0001.jpg "Western Blot: Cbx2 Antibody (3Z6W6) [NBP3-16164] - Western blot analysis of extracts of various cell lines, using Cbx2 Rabbit mAb (NBP3-16164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Cbx2 Antibody (SR2008) [NBP3-22125] - Western blot analysis using NBP3-22125 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22125_rabbit-cbx2-mab-sr2008-57202316582175.jpg "Western Blot: Cbx2 Antibody (SR2008) [NBP3-22125] - Western blot analysis using NBP3-22125 in HeLa cell lysate.")
![ELISA: Cbx2 Antibody (3E2) [H00084733-M04] - Detection limit for recombinant GST tagged CBX2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cbx2-Antibody-3E2-ELISA-H00084733-M04-img0001.jpg "ELISA: Cbx2 Antibody (3E2) [H00084733-M04] - Detection limit for recombinant GST tagged CBX2 is approximately 3ng/ml as a capture antibody.")
![Western Blot: Cbx2 Antibody (24A2) [NBP3-26694] - Positive Western Blot detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Rat heart tissue. All lanes: Cbx2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 24 kDa Observed band size: 60-72 kDa](http://images.novusbio.com/fullsize/nbp3-26694_rabbit-cbx2-mab-24a2-28220241140919.jpg "Western Blot: Cbx2 Antibody (24A2) [NBP3-26694] - Positive Western Blot detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Rat heart tissue. All lanes: Cbx2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 24 kDa Observed band size: 60-72 kDa")
![Western Blot: CBX2 Overexpression Lysate (Adult Normal) [NBL1-08747] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CBX2.](http://images.novusbio.com/fullsize/CBX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08747-img0002.jpg "Western Blot: CBX2 Overexpression Lysate (Adult Normal) [NBL1-08747] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CBX2.")