| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82849-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76619-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16925-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56083-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76430-0.025mg | |||
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0005.jpg "Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
![Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Immunohistochemistry-Paraffin-NBP2-67552-img0002.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0004.jpg)
![Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Tuberin on Jurkat cell lysates using anti-Tuberin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Western-Blot-NBP2-67552-img0003.jpg)
![Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Hela cells with Tuberin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSC2-Antibody-SC05-59-Flow-Cytometry-NBP2-67552-img0001.jpg)
![Western Blot: TSC2 Antibody [NBP1-82849] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/TSC2-Antibody-Western-Blot-NBP1-82849-img0008.jpg "Western Blot: TSC2 Antibody [NBP1-82849] - Analysis in human cell line SH-SY5Y.")
![Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP1-82849] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TSC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82849-img0007.jpg)
![Immunohistochemistry-Paraffin: TSC2 Antibody [NBP1-82849] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/TSC2-Antibody-Immunohistochemistry-Paraffin-NBP1-82849-img0006.jpg)
![Western Blot: TSC2 Antibody [NBP1-76619] - Analysis of TSC2 in L1210 cell lysate with TSC2 antibody at (A) 2 and (B) 4 ug/mL.](http://images.novusbio.com/fullsize/TSC2-Antibody-Western-Blot-NBP1-76619-img0005.jpg "Western Blot: TSC2 Antibody [NBP1-76619] - Analysis of TSC2 in L1210 cell lysate with TSC2 antibody at (A) 2 and (B) 4 ug/mL.")
![Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP1-76619] - L1210 cells with TSC2 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TSC2-Antibody-Immunocytochemistry-NBP1-76619-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TSC2 Antibody - BSA Free [NBP1-76619] - Immunofluorescence of TSC2 in L1210 cells with TSC2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76619_rabbit-polyclonal-tsc2-antibody-2711202420441185.jpg)
![Western Blot: TSC2 Antibody (S06-0D3) [NBP3-14981] - Western blot detection of TSC2 in Hela, A549, MCF-7, 293T cell lysates using TSC2 Rabbit mAb (1:1000 diluted). Predicted band size: 201kDa. Observed band size: 201kDa.](http://images.novusbio.com/fullsize/TSC2-Antibody-S06-0D3-Western-Blot-NBP3-14981-img0001.jpg "Western Blot: TSC2 Antibody (S06-0D3) [NBP3-14981] - Western blot detection of TSC2 in Hela, A549, MCF-7, 293T cell lysates using TSC2 Rabbit mAb (1:1000 diluted). Predicted band size: 201kDa. Observed band size: 201kDa.")
![Immunohistochemistry: TSC2 Antibody (S06-0D3) [NBP3-14981] - Immunohistochemical of TSC2 in Human hepatic carcinoma tissue using TSC2 antibody at dilution 1/50](http://images.novusbio.com/fullsize/TSC2-Antibody-S06-0D3-Immunohistochemistry-NBP3-14981-img0002.jpg)
![Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/TSC2-[p-Ser1387]-Antibody-Western-Blot-NBP3-16925-img0001.jpg "Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Western-Blot-NBP3-15763-img0001.jpg "Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSC2 antibody (NBP3-15763). Western blot was performed from the immunoprecipitate using TSC2 antibody (NBP3-15763) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Immunoprecipitation-NBP3-15763-img0002.jpg)
![Recombinant Human TSC2 Protein [H00007249-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007249-Q01-1.jpg "Recombinant Human TSC2 Protein [H00007249-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32813_rabbit-tsc2-p-thr1462-mab-je43-81-288202412461624.jpg "Western Blot: TSC2 [p Thr1462] Antibody (JE43-81) [NBP3-32813] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32813) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32813) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP2-56083] - Staining of human cell line HeLa shows localization to cytosol.](http://images.novusbio.com/fullsize/TSC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56083-img0001.jpg "Immunocytochemistry/Immunofluorescence: TSC2 Antibody [NBP2-56083] - Staining of human cell line HeLa shows localization to cytosol.")
![Immunoprecipitation: TSC2 Antibody [NB200-200] - Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody NB200-200 used for IP at 3 ug per reaction. TSC2 was also immunoprecipitated by another rabbit anti-TSC2 antibody. For blotting immunoprecipitated TSC2, NB200-200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TSC2-Antibody-Immunoprecipitation-NB200-200-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: TSC2 Antibody [NB200-200] - Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody NB200-200 used for IP at 3 ug per reaction. TSC2 was also immunoprecipitated by another rabbit anti-TSC2 antibody. For blotting immunoprecipitated TSC2, NB200-200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: TSC2 Antibody [NBP1-76430] - Western blot analysis of TSC2 in L1210 cell lysate with anti-TSC2 (CT) using IMG-5540 at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/TSC2-Antibody-Western-Blot-NBP1-76430-img0001.jpg "Western Blot: TSC2 Antibody [NBP1-76430] - Western blot analysis of TSC2 in L1210 cell lysate with anti-TSC2 (CT) using IMG-5540 at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.")
![Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32812_rabbit-tsc2-p-ser939-mab-je54-06-28820241244294.jpg "Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 201 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")