HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="ZEB1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB11504) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11504-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21854-50ul | |||
HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # ![Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-298202414262614.jpg "Immunohistochemistry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ZEB1 antibody (NBP3-33133) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33133) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: ZEB1 Antibody (PSH0-61) [NBP3-33133] - Flow cytometric analysis of Jurkat cells labeling ZEB1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33133, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33133_rabbit-zeb1-mab-psh0-61-2920241645327.jpg)
![Western Blot: ZEB1 Antibody (JE57-64) [NBP3-33111] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/500 dilution.<br/><br/> Lane 1: Hela cell lysate<br/> Lane 2: HUVEC cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 124 kDa<br/> Observed band size: 200 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33111_rabbit-zeb1-mab-je57-64-288202414191510.jpg "Western Blot: ZEB1 Antibody (JE57-64) [NBP3-33111] - Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/500 dilution.<br/><br/> Lane 1: Hela cell lysate<br/> Lane 2: HUVEC cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 124 kDa<br/> Observed band size: 200 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZEB1 Antibody (JE57-64) [NBP3-33111] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-ZEB1 antibody (NBP3-33111) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33111) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33111_rabbit-zeb1-mab-je57-64-29820241492615.jpg)
![Western blot analysis on different lysates with NBP3-33111 at 1/500 dilution. Lane 1: HUVEC cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33111 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33111_-western-blot-639204594554721976.jpg)
![Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunoprecipitation-NBP3-14694-img0001.jpg "Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.")
![Western Blot: ZEB1 Antibody (SR1312) [NBP3-21854] - Western blot analysis using NBP3-21854 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21854_rabbit-zeb1-mab-sr1312-5720231754860.jpg "Western Blot: ZEB1 Antibody (SR1312) [NBP3-21854] - Western blot analysis using NBP3-21854 in HEK293 cell lysate.")
![SDS-Page: Recombinant Human ZEB1 Protein [H00006935-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006935-Q01-1.jpg "SDS-Page: Recombinant Human ZEB1 Protein [H00006935-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: ZEB1 Overexpression Lysate (Adult Normal) [NBL1-18010] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZEB1.](http://images.novusbio.com/fullsize/ZEB1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-18010-img0002.jpg "Western Blot: ZEB1 Overexpression Lysate (Adult Normal) [NBL1-18010] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZEB1.")
