HAF007). A specific band was detected for VAMP-7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP-7 Monoclonal Antibody (Catalog # MAB6117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6117-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15595-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41183-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80709-25ul | |||
![Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Western-Blot-NBP1-07118-img0002.jpg "Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of MDCK cells (red). GFP-Tagged human Ti-VAMP (green) and merge (yellow).](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Immunocytochemistry-Immunofluorescence-NBP1-07118-img0001.jpg)
![Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of human spleen.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Immunohistochemistry-Paraffin-NBP1-07118-img0003.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Western-Blot-NBP2-32232-img0001.jpg)
![Immunoprecipitation: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for IP at 6 ug per reaction. VAMP7 was also immunoprecipitated by rabbit anti-VAMP7 antibody BL16273. For blotting immunoprecipitated VAMP7, NBP2-32232 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunoprecipitation-NBP2-32232-img0002.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - VAMP7 forms a SNARE complex with SNAP-23 & Syntaxin11. (A) Model illustrating the formation of SNARE complex between VAMP7 (v-SNARE), SNAP-23, & Syntaxin11 (t- SNARE) during cytotoxic granule fusion in human CD8+ T cells. (B) Different Twin-Strep-tag fusion constructs used for pulldown assay. Twin-Strep-tag was fused at the C terminus of VAMP7 & at the N terminus of Syntaxin11 with a (GGS)x3 linker. (C) Western blot of bead stimulated human CD8+ T cells transfected with Twin-Strep-tag-tagged VAMP7, immuno-precipitated with anti-FLAG antibody & detected with antibodies against FLAG, SNAP-23, & Syntaxin11. (D) Western blot of bead stimulated human CD8+ T cells transfected with Twin-Strep-tag-tagged Syntaxin11, immuno-precipitated with anti-Syntaxin antibody & detected with antibodies against Strep-tag, SNAP-23, VAMP7, & VAMP2. As control, cells were transfected with Twin-Strep-tag construct. Ten percentage of the lysates were loaded as input. (E,F) Densitometric quantification of the Western blots shown in (C,D), respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416114749.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - Knockdown of VAMP7 strongly reduces fusion of cytotoxic granules at the IS. (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) & blotted for VAMP7 (top) & GAPDH (bottom) as loading control. (B) Quantification of VAMP7 protein expression (in % normalized to control siRNA-treated CTLs) performed by densitometry. Bars indicate SEMs. [VAMP7-siRNA1, N = 3; ***p < 0.001 & VAMP7-siRNA2, N = 3; ***p < 0.001 (t-test)]. (C) Human CD8+ T cells co-transfected with granzyme B-mCherry along with either ns-siRNA or VAMP7-siRNA1 or VAMP7-siRNA2 & imaged 12 h after transfection. Selected live-cell TIRF microscopy images of granzyme B-mCherry in a transfected CTL in contact with an anti-CD3 coated coverslip. Fusion events are indicated with open circles (three frames shown per granule fused). (D) Mean percentage of cytotoxic granule fusion in cells transfected with either ns-siRNA (n = 66 & n = 59, respectively) or VAMP7-siRNA1 [n = 91; **p < 0.01 (t-test)] or VAMP7-siRNA2 [n = 72; ***p < 0.001 (t-test)]. (E) Mean average number of granules fused over time in the TIRF plane per cell p = 0.206 (t-test) for VAMP7-siRNA1 & **p < 0.01 (t-test) for VAMP7-siRNA2. Bars indicate mean ± SEM. Scale bar, 5 μm. (F) Calcein-based killing assay for CTLs transfected with either ns-siRNA, VAMP7-siRNA1, or VAMP7-siRNA2. Experiments were carried out in duplicate [N = 4; ***p < 0.001 (t-test)]. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416121985.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - Fusion of cytotoxic granules with the plasma membrane is insensitive to Tetanus toxin. (A) Bead-stimulated human CD8+ T cells transfected with GFP or TeNT-GFP as indicated. VAMP2 & VAMP7 protein levels were determined by Western blot analysis 12–16 h after transfection. (B) Expression of VAMP2 & VAMP7 protein levels relative to GAPDH in CTLs transfected with GFP or TeNT-GFP as indicated. Graphs represent means [N = 3, ***p < 0.001 (Student's t-test)]. (C) Bead-stimulated human CTLs co-transfected with either GFP or TeNT-GFP along with granzyme B-mCherry & imaged 12 h after transfection. Representative live-cell TIRFM images of CTLs in contact with an anti-CD3 coated coverslip. Fusion events indicated with open arrowheads. (D) Mean percentage of cytotoxic granule fusion in cells transfected with either GFP (n = 50) or TeNT-GFP (n = 40), p = 0.704 (Student's t-test). (E) Mean average number of granules fused per cell over time in the TIRF plane in cells transfected with either GFP (n = 20) or TeNT-GFP (n = 15), p = 0.939 (Student's t-test). Bars show mean ± SEM. Scale bar, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416114764.jpg)
![Western Blot: VAMP-7 Antibody [NBP3-15595] - analysis of various lysates, using VAMP7 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15595_rabbit-vamp-7-pab-western-blot-1411202382753..jpg "Western Blot: VAMP-7 Antibody [NBP3-15595] - analysis of various lysates, using VAMP7 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: VAMP-7 Antibody [NBP3-15595] -analysis of Mouse brain, using VAMP7 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/antibody/nbp3-15595_rabbit-vamp-7-pab-western-blot-141120238298..jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP3-15595] - Immunofluorescence analysis of C6 cells using VAMP-7 antibody (NBP3-15595) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15595-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP3-15595] - Immunofluorescence analysis of U2OS cells using VAMP-7 antibody (NBP3-15595) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15595-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-7 Antibody - Azide and BSA Free [VAMP-7] - Immunofluorescence analysis of NIH/3T3 cells using VAMP-7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15595_rabbit-vamp-7-pab-5220251731101.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-41183] - Analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Western-Blot-NBP2-41183-img0003.jpg "Western Blot: VAMP-7 Antibody [NBP2-41183] - Analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: VAMP-7 Antibody - BSA Free [NBP2-41183] - Immunofluorescence of VAMP-7 in human lung tissue with VAMP-7 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41183_rabbit-polyclonal-vamp-7-antibody-2711202420405590.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP1-80709] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80709-img0006.jpg "Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP1-80709] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![SDS-Page: VAMP7 Protein [NBP2-22939]](http://images.novusbio.com/fullsize/VAMP7-Protein-SDS-Page-NBP2-22939-img0001.jpg "SDS-Page: VAMP7 Protein [NBP2-22939]")
![SDS-Page: Recombinant Mouse VAMP-7 His Protein [NBP2-59576] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Mouse-VAMP-7-His-Protein-SDS-Page-NBP2-59576-img0001.jpg "SDS-Page: Recombinant Mouse VAMP-7 His Protein [NBP2-59576] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
