| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52423-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1128-BM-010/CF | |||
5020-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB50201). The ND50 is typically 0.4-2.4 µg/mL." alt="Recombinant Mouse BMP-4 (Catalog # 5020-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB50201). The ND50 is typically 0.4-2.4 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB50201-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21639-50ul | |||
NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. " alt="BMP-4 was detected in immersion fixed HepG2 human hepatocytoma cell line (positive) or K562 human myelogenous leukemia cell line (negative) using Rabbit Anti-Human BMP-4 Monoclonal Antibody (Catalog # MAB7572) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7572-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70256 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26476-50ul | |||
![Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa).](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11H8-Western-Blot-NBP2-52423-img0006.jpg "Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa).")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis of Hela cells using BMP-4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11H8-Immunocytochemistry-Immunofluorescence-NBP2-52423-img0004.jpg)
![Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11H8-Western-Blot-NBP2-52423-img0003.jpg)
![Western Blot: BMP-4 Antibody (3C11H8) [NBP2-52423] - Analysis using BMP4 mouse mAb against A549 (1) and C6 (2) cell lysate.](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11H8-Western-Blot-NBP2-52423-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11H8) [NBP2-52423] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11H8-Immunofluorescence-NBP2-52423-img0001.jpg)
![ELISA: BMP-4 Antibody (3C11H8) [NBP2-52423] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/BMP-4-Antibody-3C11H8-ELISA-NBP2-52423-img0007.jpg)
![Western Blot: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Analysis of extracts of various cell lines, using BMP-4 Rabbit mAb (NBP3-15376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/BMP-4-Antibody-7Q2U7-Western-Blot-NBP3-15376-img0003.jpg "Western Blot: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Analysis of extracts of various cell lines, using BMP-4 Rabbit mAb (NBP3-15376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Immunofluorescence analysis of HeLa cells using BMP-4 Rabbit mAb (NBP3-15376) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/BMP-4-Antibody-7Q2U7-Immunocytochemistry-Immunofluorescence-NBP3-15376-img0002.jpg)
![Immunohistochemistry-Paraffin: BMP-4 Antibody (7Q2U7) [NBP3-15376] - Human colon using BMP-4 Rabbit mAb (NBP3-15376) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BMP-4-Antibody-7Q2U7-Immunohistochemistry-Paraffin-NBP3-15376-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (7Q2U7) [BMP-4] - Confocal imaging of HeLa cells using BMP-4 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-15376_rabbit-bmp-4-mab-7q2u7-52202517172856.jpg)
![Immunohistochemistry: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling BMP-4 with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32086, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-29820241420987.jpg "Immunohistochemistry: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling BMP-4 with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32086, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-29820241420934.jpg)
![Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (JE42-44) [NBP3-32086] - Immunocytochemistry analysis of HeLa cells labeling BMP-4 with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BMP-4 antibody (NBP3-32086) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32086_rabbit-bmp-4-mab-je42-44-29202410484832.jpg)
![Immunohistochemistry: BMP-4 Antibody (SR1811) [NBP3-21639] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21639.](http://images.novusbio.com/fullsize/nbp3-21639_rabbit-bmp-4-mab-sr1811-57202316494286.jpg "Immunohistochemistry: BMP-4 Antibody (SR1811) [NBP3-21639] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21639.")
![Western Blot: BMP-4 Antibody (SR1811) [NBP3-21639] - Western blot analysis using NBP3-21639 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21639_rabbit-bmp-4-mab-sr1811-572023152612.jpg)
![Western Blot: BMP-4 Antibody (6B7) [NBP1-47666] - BMP4 antibody ( 6B7 ) at 1:100000 dilution + Recombinant human BMP4.](http://images.novusbio.com/fullsize/BMP-4-Antibody-6B7-Western-Blot-NBP1-47666-img0004.jpg "Western Blot: BMP-4 Antibody (6B7) [NBP1-47666] - BMP4 antibody ( 6B7 ) at 1:100000 dilution + Recombinant human BMP4.")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (6B7) [NBP1-47666] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY BMP4.](http://images.novusbio.com/fullsize/BMP-4-Antibody-6B7-Immunocytochemistry-Immunofluorescence-NBP1-47666-img0003.jpg)
![Western Blot: BMP-4 Antibody (19B7) [NBP3-26476] - Positive Western Blot detected in: Hela whole cell lysate.<br /> All lanes: BMP-4 Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 47 kDa<br /> Observed band size: 36-55 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26476_rabbit-bmp-4-mab-19b7-282202411472124.jpg "Western Blot: BMP-4 Antibody (19B7) [NBP3-26476] - Positive Western Blot detected in: Hela whole cell lysate.<br /> All lanes: BMP-4 Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 47 kDa<br /> Observed band size: 36-55 kDa<br />")
![Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (19B7) [NBP3-26476] - Staining of Hela cell with BMP-4 Antibody (19B7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 508-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26476_rabbit-bmp-4-mab-19b7-2822024132363.jpg)
![SDS-Page: Recombinant Human BMP-4 Protein [NBC1-19056] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-BMP-4-Protein-SDS-Page-NBC1-19056-img0001.jpg "SDS-Page: Recombinant Human BMP-4 Protein [NBC1-19056] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
![ELISA: Rat BMP-4 ELISA Kit (Colorimetric) [NBP2-82159] - Samples were spiked with high concentrations of Rat BMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82159_rat-bmp-4-elisa-kit-colorimetric-132202416225285.jpg "ELISA: Rat BMP-4 ELISA Kit (Colorimetric) [NBP2-82159] - Samples were spiked with high concentrations of Rat BMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat BMP-4 ELISA Kit (Colorimetric) [NBP2-82159] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82159_rat-bmp-4-elisa-kit-colorimetric-4320249124824.jpg)
![ELISA: Mouse BMP-4 ELISA Kit (Colorimetric) [NBP2-82123] - Samples were spiked with high concentrations of Mouse BMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82123_mouse-bmp-4-elisa-kit-colorimetric-132202416184885.jpg "ELISA: Mouse BMP-4 ELISA Kit (Colorimetric) [NBP2-82123] - Samples were spiked with high concentrations of Mouse BMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse BMP-4 ELISA Kit (Colorimetric) [NBP2-82123] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82123_mouse-bmp-4-elisa-kit-colorimetric-432024856725.jpg)
