Immunoblot Buffer Group 1." alt="Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat WNK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WNK1 at approximately 230 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2849-SP | |||
HAF008). A specific band was detected for Phospho-WNK1 (T60) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 1 µg/mL insulin for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-WNK1 (T60) Monoclonal Antibody (Catalog # MAB4720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-WNK1 (T60) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4720-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32434-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50364-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26251-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58438-25ul | |||
![Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25748_rabbit-wnk1-mab-hl2184-azide-and-bsa-free-2120249371520.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25748_rabbit-wnk1-mab-hl2184-azide-and-bsa-free-2120249362711.jpg)
![Immunocytochemistry/Immunofluorescence: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25748_rabbit-wnk1-mab-hl2184-azide-and-bsa-free-212024939187.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25748_rabbit-wnk1-mab-hl2184-azide-and-bsa-free-212024942267.jpg)
![Western Blot: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25748_rabbit-wnk1-mab-hl2184-azide-and-bsa-free-2120249403819.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody (HL2184) - Azide and BSA Free [NBP3-25748] - WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. WNK1 stained by WNK1 antibody [HL2184] (NBP3-25748) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25748_rabbit-wnk1-mab-hl2184-azide-and-bsa-free-2120249351390.jpg)
![Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of non-small cell carcinoma of the lung.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-NBP3-14518-img0005.jpg "Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of non-small cell carcinoma of the lung.")
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP3-14518-img0001.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP3-14518-img0002.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, non-small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP3-14518-img0003.jpg)
![Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of brain, glioblastoma.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-NBP3-14518-img0004.jpg)
![Immunohistochemistry: WNK1 Antibody [NBP3-14518] - Immunohistochemistry of ovary carcinoma](http://images.novusbio.com/fullsize/WNK1 Antibody-Immunohistochemistry-NBP3-14518-img0006.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP2-32434] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP2-32434-img0009.jpg "Immunohistochemistry-Paraffin: WNK1 Antibody [NBP2-32434] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP2-32434] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP2-32434-img0007.jpg)
![Immunohistochemistry-Paraffin: WNK1 Antibody [NBP2-32434] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP2-32434-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: WNK1 Antibody [NBP2-32434] - Staining in human esophagus and pancreas tissues using anti-WNK1 antibody. Corresponding WNK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunohistochemistry-Paraffin-NBP2-32434-img0010.jpg)
![Western Blot: WNK1 Antibody (M42-P3B10) [NBP2-50364] - Detection of WNK1 in a HeLa cell lysate. A 250kDa protein was detected.](http://images.novusbio.com/fullsize/WNK1-Antibody-M42-P3B10-Western-Blot-NBP2-50364-img0001.jpg "Western Blot: WNK1 Antibody (M42-P3B10) [NBP2-50364] - Detection of WNK1 in a HeLa cell lysate. A 250kDa protein was detected.")
![Western Blot: WNK1 Antibody [NB100-74641] - Detection of Human WNK1 on HeLa whole cell lysate using NB100-74641. WNK1 was also immunoprecipitated by rabbit anti-WNK1 antibodies NB100-74640, NB100-74642 and NB100-74643.](http://images.novusbio.com/fullsize/WNK1-Antibody-Western-Blot-NB100-74641-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: WNK1 Antibody [NB100-74641] - Detection of Human WNK1 on HeLa whole cell lysate using NB100-74641. WNK1 was also immunoprecipitated by rabbit anti-WNK1 antibodies NB100-74640, NB100-74642 and NB100-74643.")
![Immunohistochemistry: WNK1 Antibody (26G7) [NBP3-26251] - Image of WNK1 Antibody diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.](http://images.novusbio.com/fullsize/nbp3-26251_rabbit-wnk1-mab-26g7-262202415355728.jpg "Immunohistochemistry: WNK1 Antibody (26G7) [NBP3-26251] - Image of WNK1 Antibody diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.")
![Immunohistochemistry: WNK1 Antibody (26G7) [NBP3-26251] - Image of WNK1 Antibody diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.60% DAB.](http://images.novusbio.com/fullsize/nbp3-26251_rabbit-wnk1-mab-26g7-272202485632.jpg)
![Immunocytochemistry/Immunofluorescence: WNK1 Antibody [NBP2-58438] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WNK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58438-img0001.jpg "Immunocytochemistry/Immunofluorescence: WNK1 Antibody [NBP2-58438] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.")
![Western Blot: WNK1 Antibody (6C5S6) [NBP3-16360] - Analysis of extracts of Mouse kidney, using WNK1 Rabbit mAb (NBP3-16360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/WNK1-Antibody-6C5S6-Western-Blot-NBP3-16360-img0001.jpg "Western Blot: WNK1 Antibody (6C5S6) [NBP3-16360] - Analysis of extracts of Mouse kidney, using WNK1 Rabbit mAb (NBP3-16360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
