| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49142-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6817-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6817-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97674-50ul | |||
![Western Blot: Skp2 Antibody [NBP2-49142] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SKP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP2-49142-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Skp2 Antibody [NBP2-49142] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SKP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0008.jpg)
![Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0005.jpg)
![Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0006.jpg)
![Immunohistochemistry-Paraffin: Skp2 Antibody [NBP2-49142] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunohistochemistry-Paraffin-NBP2-49142-img0007.jpg)
![Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of HeLa cells, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Western-Blot-NBP3-16320-img0002.jpg "Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of HeLa cells, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Skp2 Antibody (2L0K1) [NBP3-16320] - Immunohistochemistry of paraffin-embedded human colon using Skp2 Rabbit mAb (NBP3-16320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Immunohistochemistry-Paraffin-NBP3-16320-img0001.jpg)
![Western Blot: Skp2 Antibody (2L0K1) [NBP3-16320] - Western blot analysis of extracts of various cell lines, using Skp2 Rabbit mAb (NBP3-16320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Skp2-Antibody-2L0K1-Western-Blot-NBP3-16320-img0003.jpg)
![Western Blot: Skp2 Antibody [NBP2-56192] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP2-56192-img0002.jpg "Western Blot: Skp2 Antibody [NBP2-56192] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-56192] - Staining of human cell line PC-3 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56192-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Skp2 Antibody [NBP2-56192] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SKP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP2-56192-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (6D4) [NBP3-15203] - Immunofluorescence staining of Hela Cells with at Skp2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Skp2-Antibody-6D4-Immunocytochemistry-Immunofluorescence-NBP3-15203-img0001.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (6D4) [NBP3-15203] - Immunofluorescence staining of Hela Cells with at Skp2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Immunohistochemistry: Skp2 Antibody (6D4) [NBP3-15203] - IHC image of Skp2 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Skp2-Antibody-6D4-Immunohistochemistry-NBP3-15203-img0002.jpg)
![SDS-Page: Recombinant Human Skp2 Protein [H00006502-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Skp2-Protein-SDS-Page-H00006502-P01-img0002.jpg "SDS-Page: Recombinant Human Skp2 Protein [H00006502-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Skp2 Antibody [NBP1-30077] - Detection of Human SKP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SKP2 antibody NBP1-30077 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Skp2-Antibody-Western-Blot-NBP1-30077-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Skp2 Antibody [NBP1-30077] - Detection of Human SKP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SKP2 antibody NBP1-30077 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: Skp2 Antibody [NBP1-30077] - Detection of human SKP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKP2 antibody NBP1-30077 used for IP at 6 ug per reaction. SKP2 was also immunoprecipitated by another rabbit anti-SKP2 antibody. For blotting immunoprecipitated SKP2, NBP1-30077 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunoprecipitation-NBP1-30077-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Skp2-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-15197-img0002.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Skp2 Antibody (3C11) [NBP3-15197] - Overlay histogram showing Hela cells stained with Skp2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Skp2-Antibody-3C11-Flow-Cytometry-NBP3-15197-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody (S08-0A2) [NBP3-19917] - Immunocytochemistry of Skp2 (green) in hela using NBP3-19917 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19917_rabbit-skp2-mab-s08-0a2-11320251555918.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody (S08-0A2) [NBP3-19917] - Immunocytochemistry of Skp2 (green) in hela using NBP3-19917 at dilution 1/50, and DAPI (blue)")
![Western Blot: SKP2 Overexpression Lysate (Adult Normal) [NBL1-15995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SKP2.](http://images.novusbio.com/fullsize/SKP2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15995-img0002.jpg "Western Blot: SKP2 Overexpression Lysate (Adult Normal) [NBL1-15995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SKP2.")
![Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-97674] - Immunofluorescence staining of Skp2 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Skp2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/Skp2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97674-img0001.jpg "Immunocytochemistry/Immunofluorescence: Skp2 Antibody [NBP2-97674] - Immunofluorescence staining of Skp2 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Skp2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")