| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72144 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47443-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38297-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72144 | |||
![Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Western-Blot-NBP2-02665-img0009.jpg "Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Staining in Hela cells: red CFTR, Green GRASP65 and Blue is DAPI. Image from a verified customer review.](http://images.novusbio.com/fullsize/GRASP65-Antibody-OTI5G8-Immunocytochemistry-Immunofluorescence-NBP2-02665-img0012.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human prostate tissue using anti-GRASP65 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Immunohistochemistry-Paraffin-NBP2-02665-img0007.jpg)
![Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRASP65 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Flow-Cytometry-NBP2-02665-img0002.jpg)
![Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP65 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRASP65.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Western-Blot-NBP2-02665-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Immunocytochemistry-Immunofluorescence-NBP2-02665-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - GRASP65 Antibody (5G8) [NBP2-02665] - Golgi localization in human Wi26 cells. Works for ICC on MeOH and PFA-fixed cells 1:100-1:200 dilution WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Immunocytochemistry-NBP2-02665-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Detection in cultured pig trabecular meshwork cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/GRASP65-Antibody-OTI5G8-Immunocytochemistry-Immunofluorescence-NBP2-02665-img0011.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRASP65 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Immunohistochemistry-Paraffin-NBP2-02665-img0004.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human Kidney tissue using anti-GRASP65 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Immunohistochemistry-Paraffin-NBP2-02665-img0005.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human pancreas tissue using anti-GRASP65 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Immunohistochemistry-Paraffin-NBP2-02665-img0006.jpg)
![Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of Jurkat cells, using anti-GRASP65 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRASP65-Antibody-5G8-Flow-Cytometry-NBP2-02665-img0001.jpg)
![Western Blot: GRASP65 Antibody [NBP2-47443] - Analysis in control (vector only transfected HEK293T lysate) and GORASP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/GRASP65-Antibody-Western-Blot-NBP2-47443-img0007.jpg "Western Blot: GRASP65 Antibody [NBP2-47443] - Analysis in control (vector only transfected HEK293T lysate) and GORASP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: GRASP65 Antibody [NBP2-47443] - Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47443-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRASP65 Antibody [NBP2-47443] - Staining in human cervix, uterine and skeletal muscle tissues using anti-GORASP1 antibody. Corresponding GORASP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunohistochemistry-Paraffin-NBP2-47443-img0006.jpg)
![Immunohistochemistry: GRASP65 Antibody [NBP2-47443] - Staining of human salivary gland shows strong cytoplasmic positivity(dot-like pattern) in glandular cells.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunohistochemistry-NBP2-47443-img0002.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody [NBP2-47443] - Staining of human cervix, uterine shows high expression.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunohistochemistry-Paraffin-NBP2-47443-img0004.jpg)
![Immunohistochemistry-Paraffin: GRASP65 Antibody [NBP2-47443] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunohistochemistry-Paraffin-NBP2-47443-img0005.jpg)
![Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GRASP65-Antibody-3F2I1-Western-Blot-NBP3-16113-img0002.jpg "Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GRASP65-Antibody-3F2I1-Western-Blot-NBP3-16113-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Confocal imaging of U-2 OS cells using GRASP65 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16113_rabbit-grasp65-mab-3f2i1-7220251245347.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Confocal imaging of Hep G2 cells using GRASP65 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16113_rabbit-grasp65-mab-3f2i1-72202512435346.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody [NBP3-38297] - Confocal immunofluorescence analysis of U2OS cells using GRASP65 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38297_rabbit-grasp65-pab-912202415133437.jpg "Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody [NBP3-38297] - Confocal immunofluorescence analysis of U2OS cells using GRASP65 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: GRASP65 Antibody [NBP3-38297] - Western blot analysis of various lysates using GRASP65 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38297_rabbit-grasp65-pab-912202414213735.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody [NBP3-38297] - Immunofluorescence analysis of A549 cells using GRASP65 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38297_rabbit-grasp65-pab-91220241581223.jpg)
![Western Blot: GRASP65 Antibody [NBP2-36527] - Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36527 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Western-Blot-NBP2-36527-img0002.jpg "Western Blot: GRASP65 Antibody [NBP2-36527] - Detection of human and mouse GRASP65 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36527 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: GRASP65 Antibody [NBP2-36527] - Detection of human GRASP65 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36527 used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody NBP2-36526. For blotting immunoprecipitated GRASP65, NBP2-36527 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunoprecipitation-NBP2-36527-img0003.jpg)
![Western Blot: GRASP65 Overexpression Lysate (Adult Normal) [NBL1-11202] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRASP65.](http://images.novusbio.com/fullsize/GRASP65-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11202-img0002.jpg "Western Blot: GRASP65 Overexpression Lysate (Adult Normal) [NBL1-11202] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRASP65.")
![Immunoprecipitation: GRASP65 Antibody [NBP2-36526] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36526 used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody NBP2-36527. For blotting immunoprecipitated GRASP65, NBP2-36527 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GRASP65-Antibody-Immunoprecipitation-NBP2-36526-img0001.jpg "Immunoprecipitation: GRASP65 Antibody [NBP2-36526] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRASP65 antibody NBP2-36526 used for IP at 6 ug per reaction. GRASP65 was also immunoprecipitated by rabbit anti-GRASP65 antibody NBP2-36527. For blotting immunoprecipitated GRASP65, NBP2-36527 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")