| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB71263-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 9057-B7-01M | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 7126-B7-01M | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44113 | |||
| BSA Free | NBP2-59031-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB71267-SP | |||
| Carrier Free | FAB71267V-100UG | |||
| Carrier Free | FAB71267U-100UG | |||
| Carrier Free | FAB71267T-100UG | |||
| Carrier Free | FAB71267S-100UG | |||
| Carrier Free | FAB71267R-100UG | |||
| Carrier Free | FAB71267N-100UG | |||
| Carrier Free | FAB71267G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB71261R-SP | |||
| Carrier Free | FAB71261RV-100UG | |||
| Carrier Free | FAB71261RU-100UG | |||
| Carrier Free | FAB71261RT-100UG | |||
| Carrier Free | FAB71261RS-100UG | |||
| Carrier Free | FAB71261RR-100UG | |||
| Carrier Free | FAB71261RN-100UG | |||
| Carrier Free | FAB71261RG-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB71264-SP | |||
![Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (730823) [MAB71263] - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-730823-Flow-Cytometry-MAB71263-img0001.jpg "Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (730823) [MAB71263] - A cell surface stain was performed on U-937 cells with VISTA/B7-H5/PD-1H (730823) antibody MAB71263PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.")
![Western Blot: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Western blot analysis of VISTA/B7-H5/PD-1H on human lung tissue lysates with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33095) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33095_rabbit-vista-b7-h5-pd-1h-mab-psh0-65-288202414191520.jpg "Western Blot: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Western blot analysis of VISTA/B7-H5/PD-1H on human lung tissue lysates with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 34 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33095) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33095) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33095_rabbit-vista-b7-h5-pd-1h-mab-psh0-65-298202413585537.jpg)
![Immunocytochemistry/ Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (PSH0-65) [NBP3-33095] - Immunocytochemistry analysis of PBMC labeling VISTA/B7-H5/PD-1H with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-VISTA/B7-H5/PD-1H antibody (NBP3-33095) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33095_rabbit-vista-b7-h5-pd-1h-mab-psh0-65-2920241192393.jpg)
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP2-59031] - Staining of human tonsil shows immunoreactivity in a subset of lymphoid and epithelial cells.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-Immunohistochemistry-Paraffin-NBP2-59031-img0005.jpg "Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP2-59031] - Staining of human tonsil shows immunoreactivity in a subset of lymphoid and epithelial cells.")
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP2-59031] - Staining of human small intestine shows strong immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-Immunohistochemistry-Paraffin-NBP2-59031-img0001.jpg)
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP2-59031] - Staining of human cerebral cortex shows positivity in a subset of cells and blood vessels.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-Immunohistochemistry-Paraffin-NBP2-59031-img0002.jpg)
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP2-59031] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblast.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-Immunohistochemistry-Paraffin-NBP2-59031-img0003.jpg)
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody [NBP2-59031] - Staining of human liver shows strong positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-Immunohistochemistry-Paraffin-NBP2-59031-img0004.jpg)
