HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">
HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma/PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPR mu, PTPR kappa, LAR, PTPR sigma, PTPR delta, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma/PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for 
![Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - PTPRS monoclonal antibody (M01), clone 1H6 Western Blot analysis of PTPRS expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/PTP-sigma-PTPRS-Antibody-1H6-Western-Blot-H00005802-M01-img0001.jpg)
![Sandwich ELISA: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Detection limit for recombinant GST tagged PTPRS is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PTP-sigma-PTPRS-Antibody-1H6-Sandwich-ELISA-H00005802-M01-img0002.jpg)
![Western Blot: PTP sigma/PTPRS Antibody (1H6) [H00005802-M01] - Characterization of purified monoclonal antibodies targeting RPTP sigma .COS7 cells were transfected with either human RPTP sigma (hRPTP sigma ) or mouse RPTP sigma (HA-mRPTPs) & 24 hrs post-transfection, western blot (A) & immunoprecipitation (B) analysis was performed using the indicated purified monoclonal antibodies. Antibody 1H6 reacted with both forms was used as positive control. Actin was the loading controls. C: Differentiated SH-SY5Y cells were immunostained using the purified 1.3H12 or 4.5H5 monoclonal antibodies for RPTP sigma (green), Dapi for the nucleus (blue) & Phalloidin for actin (red). IgG was used as negative control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0178489), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005802-m01_mouse-monoclonal-ptp-sigma-ptprs-antibody-1h6-310202415291997.jpg)
![Western Blot: PTPRS Overexpression Lysate (Adult Normal) [NBL1-14988] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPRS.](http://images.novusbio.com/fullsize/PTPRS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14988-img0002.jpg "Western Blot: PTPRS Overexpression Lysate (Adult Normal) [NBL1-14988] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTPRS.")
![ELISA: Human PTP sigma/PTPRS - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40271] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40271_human-ptp-sigma-ptprs-ready-to-use-elisa-kit-color-211020241538052.png "ELISA: Human PTP sigma/PTPRS - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40271] - Standard Curve Reference")
![ELISA: Human PTP sigma/PTPRS ELISA Kit (Colorimetric) [NBP3-39065] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39065_human-ptp-sigma-ptprs-elisa-kit-colorimetric-2110202415273877.png "ELISA: Human PTP sigma/PTPRS ELISA Kit (Colorimetric) [NBP3-39065] - Standard Curve Reference")
