MAB8307) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB8307) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Human Hepcidin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog #
MAB8307) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Hepcidin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB8307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
MAB8307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Hepcidin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB8307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human Hepcidin Monoclonal Antibody (Catalog # MAB83071) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![Western Blot: Hepcidin Antimicrobial Peptide Antibody [NBP1-59337] - Human Spleen lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Hepcidin-Antimicrobial-Peptide-Antibody-Western-Blot-NBP1-59337-img0002.jpg "Western Blot: Hepcidin Antimicrobial Peptide Antibody [NBP1-59337] - Human Spleen lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody [NBP1-59337] - Paraffin embedded sections of posterior segment of mouse eye, red is Hepcidin and blue is Hoeschst. 4um sections were rehydrated with xylene, followed by a decreasing ethanol concentration gradient (100%, 90%, 70%) and a wash with diH2O. Heat-mediated antigen retrieval performed using EDTA buffer (10mM Trizma Base, 1mM EDTA solution, 0.05% Tween 20, pH 9.0) in an autoclave for 30min. Primary antibody against Hepcidin was diluted 1:10, in blocking solution containing 0.1% BSA, 0.05% Triton X-100, and 5% normal donkey serum in TBS. Sections were incubated for 48hrs at room temperature and then 24hrs at 4C. Tissues were washed with TBS-T (6x5min), and immunoreactivity for Hep was developed. Image from verified customer review.](http://images.novusbio.com/fullsize/Hepcidin-Antimicrobial-Peptide-Antibody-Immunohistochemistry-Paraffin-NBP1-59337-img0003.jpg)
![N/A Hepcidin Antimicrobial Peptide [HRP]](http://images.novusbio.com/fullsize/elisa/Hepcidin_DHP250_ELISA_1122.jpg "N/A Hepcidin Antimicrobial Peptide [HRP]")
![N/A Hepcidin Antimicrobial Peptide [HRP]](http://images.novusbio.com/fullsize/elisa/Hepcidin_DHP250_ELISA_1121.jpg)
![N/A Hepcidin Antimicrobial Peptide [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Hepcidin_DY830705_ELISA_2541.jpg "N/A Hepcidin Antimicrobial Peptide [Biotin]")
![ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Samples were spiked with high concentrations of Rat Hepcidin Antimicrobial Peptide and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82130_rat-hepcidin-antimicrobial-peptide-elisa-kit-colorimetric-132202416243426.jpg "ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Samples were spiked with high concentrations of Rat Hepcidin Antimicrobial Peptide and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82130_rat-hepcidin-antimicrobial-peptide-elisa-kit-colorimetric-432024955090.jpg)
![Immunohistochemistry: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] - Image of Hepcidin Antimicrobial Peptide Antibody (4C5) diluted at 1:215 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26242_rabbit-hepcidin-antimicrobial-peptide-mab-4c5-272202485639.jpg "Immunohistochemistry: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] - Image of Hepcidin Antimicrobial Peptide Antibody (4C5) diluted at 1:215 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate.<br /> All lanes: Hepcidin Antimicrobial Peptide Antibody at 2.15ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 10 KDa<br /> Observed band size: 25 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26242_rabbit-hepcidin-antimicrobial-peptide-mab-4c5-282202411472146.jpg)
![ELISA: Mouse Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82129] - Samples were spiked with high concentrations of Mouse Hepcidin Antimicrobial Peptide and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82129_mouse-hepcidin-antimicrobial-peptide-elisa-kit-color-132202416173421.jpg "ELISA: Mouse Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82129] - Samples were spiked with high concentrations of Mouse Hepcidin Antimicrobial Peptide and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82129] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82129_mouse-hepcidin-antimicrobial-peptide-elisa-kit-color-432024903583.jpg)
![Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody [NBP1-36960] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/Hepcidin-Antimicrobial-Peptide-Antibody-Immunohistochemistry-Paraffin-NBP1-36960-img0001.jpg "Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody [NBP1-36960] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.")
![Recombinant Human Hepcidin Antimicrobial Peptide Protein [H00057817-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00057817-P01-1.jpg "Recombinant Human Hepcidin Antimicrobial Peptide Protein [H00057817-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
