HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4499-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88157-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73708 | |||
HAF007). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4499-SP | |||
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry-Paraffin: PTK7/CCK4 Antibody [NBP1-88157] - Staining of human skin shows weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-Immunohistochemistry-Paraffin-NBP1-88157-img0012.jpg "Immunohistochemistry-Paraffin: PTK7/CCK4 Antibody [NBP1-88157] - Staining of human skin shows weak membranous positivity in squamous epithelial cells.")
![Immunohistochemistry-Paraffin: PTK7/CCK4 Antibody [NBP1-88157] - Staining of human endometrium shows strong membranous positivity in stromal cells.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-Immunohistochemistry-Paraffin-NBP1-88157-img0009.jpg)
![Immunohistochemistry-Paraffin: PTK7/CCK4 Antibody [NBP1-88157] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-Immunohistochemistry-Paraffin-NBP1-88157-img0010.jpg)
![Immunohistochemistry-Paraffin: PTK7/CCK4 Antibody [NBP1-88157] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-Immunohistochemistry-Paraffin-NBP1-88157-img0011.jpg)
![Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CCK4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CCK4-Antibody-2E7-Western-Blot-NBP2-01486-img0004.jpg "Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CCK4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CCK4.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-01486-img0005.jpg)
![Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CCK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CCK4-Antibody-2E7-Flow-Cytometry-NBP2-01486-img0001.jpg)
![Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCK4.](http://images.novusbio.com/fullsize/CCK4-Antibody-2E7-Western-Blot-NBP2-01486-img0002.jpg)
CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for 
![Western Blot: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Analysis of PTK7 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-4F5-Western-Blot-H00005754-M06-img0006.jpg "Western Blot: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Analysis of PTK7 expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Analysis of monoclonal antibody to PTK7 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-4F5-Immunocytochemistry-Immunofluorescence-H00005754-M06-img0004.jpg)
![ELISA: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Detection limit for recombinant GST tagged PTK7 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-4F5-ELISA-H00005754-M06-img0005.jpg)
![Western Blot: PTK7/CCK4 Antibody (4F9) [NBP1-51633] - Analysis using PTK7 mouse mAb against Hela (1), A431 (2), HCT116 (3), Caco2 (4), HepG2 (5) and MCF-7 (6) cell lysate.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-4F9-Western-Blot-NBP1-51633-img0005.jpg "Western Blot: PTK7/CCK4 Antibody (4F9) [NBP1-51633] - Analysis using PTK7 mouse mAb against Hela (1), A431 (2), HCT116 (3), Caco2 (4), HepG2 (5) and MCF-7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: PTK7/CCK4 Antibody (4F9) [NBP1-51633] - Analysis of lung cancer tissues using PTK7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PTK7-CCK4-Antibody-4F9-Immunohistochemistry-Paraffin-NBP1-51633-img0004.jpg)
![Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-29135] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-29135 (lot NBP3-29135-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-29135 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29135_rabbit-ptk7-cck4-pab-3012202420334519.jpg "Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-29135] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-29135 (lot NBP3-29135-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-29135 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-30221] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-30221 (lot NBP3-30221-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-30221 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30221_rabbit-ptk7-cck4-pab-301220242046548.jpg "Immunoprecipitation:PTK7/CCK4 AntibodyNBP3-30221] -Detection of human PTK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PTK7 antibody NBP3-30221 (lot NBP3-30221-1) used for IP at 6 µg per reaction. PTK7 was also immunoprecipitated by rabbit anti-PTK7.. For blotting immunoprecipitated PTK7, NBP3-30221 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
