| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77310-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77311-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14462-25ul | |||
NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Endosialin/CD248 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7535-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99057-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72075 | |||
![Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 4357, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77310-img0006.jpg "Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 4357, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human Colon tissue with TEM1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Immunofluorescence-NBP1-77310-img0003.jpg)
![Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77310-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 (NBP1-77310), (2 ug/mL), TEM-1 (NBP1-77311), (1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77310-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310] - <strong>Figure 5 Immunofluorescence Validation of TEM-1 in Human Colon Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77310_rabbit-polyclonal-endosialin-cd248-tem1-antibody-2711202420175151.jpg)
![Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77310] - Validation of TEM-1 in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77310_rabbit-polyclonal-endosialin-cd248-tem1-antibody-271120242004150.jpg)
![Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0005.jpg "Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77311, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77311] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 NBP1-77310, (2 ug/mL), TEM-1 NBP1-77311, ( 1ug/mL), and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Western-Blot-NBP1-77311-img0004.jpg)
![Immunohistochemistry: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] - Figure 4 Immunohistochemistry Validation of TEM-1 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77311_rabbit-polyclonal-endosialin-cd248-tem1-antibody-271120241932644.jpg)
![Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody - BSA Free [NBP1-77311] - Validation of TEM-1 in Human Colon Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77311_rabbit-polyclonal-endosialin-cd248-tem1-antibody-271120242004185.jpg)
![Immunocytochemistry/Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14462-img0012.jpg "Immunocytochemistry/Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining in human placenta and tonsil tissues using anti-CD248 antibody. Corresponding CD248 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Immunohistochemistry-Paraffin-NBP2-14462-img0010.jpg)
![Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Immunohistochemistry-Paraffin-NBP2-14462-img0008.jpg)
![Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Immunohistochemistry-Paraffin-NBP2-14462-img0009.jpg)
NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for ![Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-99057] - Staining of mouse Endosialin/CD248/TEM1 in mouse pancreas with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-Immunohistochemistry-Paraffin-NBP2-99057-img0001.jpg "Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-99057] - Staining of mouse Endosialin/CD248/TEM1 in mouse pancreas with rabbit polyclonal antibody (1:1000).")
![Western Blot: Endosialin/CD248/TEM1 Antibody (4B8) [NBP2-46129] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD248 .](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-4B8-Western-Blot-NBP2-46129-img0001.jpg "Western Blot: Endosialin/CD248/TEM1 Antibody (4B8) [NBP2-46129] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD248 .")
![Western Blot: TEM1 Overexpression Lysate (Adult Normal) [NBP2-06637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TEM1.](http://images.novusbio.com/fullsize/TEM1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06637-img0001.jpg "Western Blot: TEM1 Overexpression Lysate (Adult Normal) [NBP2-06637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TEM1.")
![Sandwich ELISA: Endosialin/CD248/TEM1 Antibody (2A7) [H00057124-M12] - Detection limit for recombinant GST tagged CD248 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Endosialin-CD248-TEM1-Antibody-2A7-Sandwich-ELISA-H00057124-M12-img0001.jpg "Sandwich ELISA: Endosialin/CD248/TEM1 Antibody (2A7) [H00057124-M12] - Detection limit for recombinant GST tagged CD248 is 0.3 ng/ml as a capture antibody.")
