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![Western Blot: DHX33 Antibody [NB100-2581] - Detection of Human and Mouse DHX33 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX33 antibody NB100-2581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DHX33-Antibody-Western-Blot-NB100-2581-img0004.jpg "Western Blot: DHX33 Antibody [NB100-2581] - Detection of Human and Mouse DHX33 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX33 antibody NB100-2581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DHX33 Antibody [NB100-2581] - Detection of human DHX33 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2581 (lot 2) used for IP at 6 ug per reaction. DHX33 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-DHX33 antibody NB100-2580. For blotting immunoprecipitated DHX33, NB100-2581 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunoprecipitation-NB100-2581-img0005.jpg)
![Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunohistochemistry-NB100-2580-img0007.jpg "Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).")
![Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection by DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunohistochemistry-NB100-2580-img0004.jpg)
![Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunohistochemistry-NB100-2580-img0005.jpg)
![Immunohistochemistry-Paraffin: DHX33 Antibody [NB100-2580] - Human lung carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunohistochemistry-Paraffin-NB100-2580-img0003.jpg)
![Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunohistochemistry-NB100-2580-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DHX33 Antibody [NB100-2580] - Detection of Human DHX33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2580 used for IP at 3 mcg/mg lysate. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody NB100-2581. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DHX33-Antibody-Immunoprecipitation-NB100-2580-img0002.jpg)