Axl: Novus Biologicals

NBP3-21382PEP

Axl Recombinant Protein Antigen

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

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NBP1-83073PEP

Axl Recombinant Protein Antigen

0.1 ml

Reactivity:

Hu

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Reactivity: Hu

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AF154

Axl Antibody [Unconjugated]

(2 Reviews)

(122 Publications)

100 μg

AF154

HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2." class="big_thumb" />

AB-108-C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (<a class=

AB-108-C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (<a class=

F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Axl at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>TGF beta promotes HLA-ABC downregulation at baseline and in response to IFN gamma .a IFN gamma -mediated induction (IFN gamma -treated/vehicle-treated control) of cell surface HLA-ABC in MITFhigh/AXLlow or /MITFlow/AXLhigh short-term PD-1 PROG melanoma cell lines. Each dot represents one cell line and HLA-ABC induction was measured by flow cytometry 24 h after treating cultures with vehicle control or 1000 U/ml IFN gamma . Box plots show the median and interquartile ranges, and data were compared using Mann-Whitney test. b Cell surface expression (median fluorescence intensity; MFI) of HLA-ABC in WMD-084, SCC14-0257 and SMU17-0132 melanoma cells treated with vehicle (Control), 1000 U/ml IFN gamma and/or 10 ng/ml TGF beta for 72 h. Data (mean ± s.d.) were compared using one-way ANOVA with the Geisser-Greenhouse correction. c Expression of de-differentiation markers AXL, N-cadherin and SNAIL in WMD-084, SCC14-0257 and SMU17-0132 melanoma cells treated with vehicle (Control), 1000 U/ml IFN gamma - and/or 10 ng/ml TGF beta for 72 h. d IFN gamma production after co-culture of TGF beta pre-treated (10 ng/ml for 72 h) melanoma cells with the patient-matched tumor-infiltrating lymphocytes expanded from the same tumor biopsy. IFN gamma was measured by flow cytometry. Data (mean ± s.d.) show relative IFN gamma expression in T cells (TGF beta pre-treated/BSA pre-treated) after background subtraction (spontaneous IFN gamma production on immune cell-only cultures). Paired BSA-treated vs TGF beta -treated data were compared using paired t-test, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32312968), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Tyro3 modulates MAPK and PI(3)K signaling pathways.As shown in panel A, Rat/T3V5 (Rat/T3, lanes 1–3) and Rat2 cells (Rat, lanes 4–6) were treated with 0, 50, and 350 ng/ml of Gas6 for 20 min. Detergent cell extracts normalized for protein concentration were separated by SDS-PAGE using 4–20% gels. Western blotting was performed with antibodies directed against alpha -Tyro3, alpha -Axl, alpha -pAKT, alpha pp70S6K, alpha -pmTOR, and alpha -pERK1/2. The membranes were stripped or cut and reprobed with alpha -ERK1/2 or alpha -GAPDH and shown beneath each panel as protein loading control. These blots are representative of 5 experiments. As shown in panel B, transiently transfected Rat2/Axl cells (Rat/Axl, lanes 1–3) and Rat2 cells (Rat, lanes 4–6) were treated and processed as above. Western blotting was performed with alpha -Axl, alpha -pAKT, and alpha -pERK1/2. The membranes were cut and reprobed with alpha -GAPDH shown beneath each panel as protein loading control. These blots are representative of 4 experiments. The total levels of MAPK and PI(3)K signaling pathway molecules was compared in Rat2 cells overexpressing Tyro3 (panel C) and Axl (panel D). For Tyro3 overexpressing cells (panel C), Rat/T3V5 cells (Rat/T3, lanes 1–3), and Rat2 untransfected cells (Rat, lanes 4–6), were treated with 0, 50 and 350 ng/ml of Gas6 for 20 min. Detergent cell extracts normalized for protein concentration were separated by SDS-PAGE using 4–20% gels. Western blotting was performed with antibodies directed against alpha -AKT, alpha p70S6K, alpha -mTOR. The membranes were cut and reprobed with alpha -GAPDH shown beneath each panel as protein loading control. For total levels of Tyro3, Axl, and ERK1/2 in Rat2 and Rat2/T3V5 cells, see Fig. 3 A and B. These blots are representative of 4 experiments. For Axl overexpressing cells (panel D), transiently transfected Rat2/Axl cells (Rat/Axl, lanes 1–3) and Rat2 untransfected cells (Rat, lanes 4–6) were treated and processed as above. Western blotting was performed with alpha -Axl, alpha -AKT, and alpha -ERK1/2. The membranes were cut and

Obesity is associated with increased frequency of stem cell-like cancer cells in PM/ER−/PR− breast cancer patients and mouse models of breast cancer.a Tumor incidence following orthotopic implantation of cancer cells into the mammary fat pads of chow- and HFD-fed mice. b Kaplan–Meier curves show disease-specific survival for PM/ER−/PR− patients (n = 48) with high (red) or low (blue) BMI. Log-rank (Mantel–Cox) P value is denoted for difference in disease-specific survival. c, d Representative tissue microarray and QuPath analysis mask pictures of CD133 (c) and Axl (d) staining in high (BMI > 25, n = 23 for CD133; n = 21 for Axl) or low (BMI ≤ 25, n = 13 for CD133; n = 11 for Axl) BMI PM/ER−/PR− patients’ tumor samples, stroma is marked in green, CD133− or Axl−/low cancer cells are marked in blue and positive staining cancer cells are marked in red (for c, P = 0.0245; d, P = 0.023). e Time-dependent proliferation assay of ex vivo E0771 cells isolated from chow or HFD-fed mice. For each time point, data are represented as mean ± SEM of four tumor samples (eight replicates per tumor sample were measured) from each group. f Tumorsphere formation assay of E0771 ex vivo cells isolated from chow diet or HFD-fed mice. Representative images of day-5 tumorspheres formation of E0771 ex vivo cells and red arrowheads mark the identified tumorspheres. Quantification of day-5 tumorspheres is represented as mean ± SEM of four tumor samples from each group and three replicates were measured for each sample, P = 0.0002. g Fatty acid and glucose oxidation on E0771 ex vivo cells isolated from two chow diet-fed mice and three HFD-fed mice. The oxidation data are normalized to cell protein content. For each sample, 8 replicates are measured and data are represented as mean ± SEM of all replicates from each group. Fatty acid oxidation P = 0.0002, Glucose oxidation P = 0.0365. For c, d, unpaired, two-tailed Welch’s t-test was used for statistical testing. For f, g, statistical significance

Expression of Axl and Gas6 in clinical samples. (A,B) Immunohistochemical analysis of Axl (A) or Gas6 (B) in non-small cell lung cancer tissues from patients who underwent surgery following preoperative chemotherapy or chemoradiotherapy. Insets show higher magnification of the areas indicated in the boxes. Scale bar, 50 μm. (A) Representative images showing tumor Axl-negative tumor tissues (left), and tumor Axl-positive tumor tissues (right). Tissues were stained with an anti-Axl antibody (brown) and counterstained with hematoxylin. (B) Representative images showing stromal Gas6-negative tumor tissues (left) and stromal Gas6-positive tumor tissues (right). Tissues were stained with an anti-Gas6 antibody (brown) and counterstained with hematoxylin. (C) Correlation between tumor Axl expression and stromal Gas6 expression in tumor tissues observed in (A and B). (D) Correlation between tumor Axl, stromal Gas6 expression and survival. Kaplan–Meier plot of disease-free survival in patients with non-small cell lung cancer who underwent surgery following preoperative chemotherapy or chemoradiotherapy, stratified according to tumor Axl and stromal Gas6 expression. Five-year disease-free survival in the patients with tumors expressing both tumor Axl and stromal Gas6 (n = 37) was significantly worse than in the both-negative group (n = 12) (21.9% vs 51.3%, p = 0.04). The five-year disease-free survival of tumor Axl-negative and stromal Gas6-positive group was 50.7%, and the difference in survival between this group and both-positive or both-negative group was not significant (p = 0.20 and 0.49, respectively); *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28878389), licensed under a CC-BY license. Not internally tested by R&D Systems.

rVSV∆G/EBOV entry kinetics: (A) VeroS cells were infected with rVSV∆G/EBOV at multiple MOIs, inoculum was removed after 1 h, and monitored for luciferase production over time. (B) VeroS, HEK293T, and HAP1 cells were infected with rVSV∆G/EBOV (MOI 1), inoculum was not removed, and monitored for luciferase production over time. Right panel zooms in to display the signals observed in HAP1 and HEK293T cells. (C) Immunoblots demonstrating production of the transfected receptors in HEK293T cells. (D) HEK293T cells transfected with the indicated receptors were infected with rVSV∆G/EBOV and monitored for luciferase production over time. Each experiment was repeated in duplicate, three independent times. Data shown are the averages of averages and corresponding SEM of three independent trials. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33348746), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Tyro3 increases Gas6-induced Axl phosphorylation.Rat2 (Rat lanes 1–3) and Rat2/T3V5 cells (Rat/T3, lanes 4–6) were treated with 0, 50, 350 ng/ml Gas6 for 20 min. Detergent cell lysates were prepared and normalized for protein concentration. The samples were divided in two for Tyro3 (panel A) and Axl (panel B) immunoprecipitations (IP). This was followed by SDS-PAGE using 8% gels followed and Western blot analysis. The membranes were probed with anti-phosphotyrosine ( alpha -pTyr) antibodies (PY20 and P99 mixture 1∶3,500) (top, panels A and B). The membranes were stripped and reprobed with alpha -Tyro3 serum 5424 (1∶3,500) (A, bottom panel) or affinity purified rabbit alpha -Axl (1∶3,500) (B, bottom panel). These blots are representative of 4 experiments. To determine whether Tyro3 expression affects Axl levels in Rat2 cells (panel C), detergent cell extracts were prepared from Rat2 cells (lane 1), and independently derived stably transfected Rat2/T3V5 cell lines (clone (cl) 25, lane 2; clone 11, lane 3; clone 30, lane 4). SDS-PAGE using 4–20% gels followed by Western blot analysis was performed on these extracts. The membranes were cut at the level of the 66 kDa marker and the top portion was probed with rabbit alpha -Tyro3 (5424 serum 1∶3,500) or rabbit alpha -Axl (1∶3,500). The bottom portion of the membranes were blotted with alpha -GAPDH (1∶500). These blots are representative of 5 experiments. To determine if the levels of Axl phosphorylation depended on the levels of Tyro3 expressed (panel D) Rat2 cells (lanes 1 and 2) and Rat2/T3V5 cell lines cl25 (lanes 3 and 4) and cl30 (lanes 5 and 6) were activated with media only (0) or 350 ng/ml of Gas6 for 10 min. Detergent cell lysates were prepared and normalized for protein concentration. The samples were divided in two for Tyro3 and Axl immunoprecipitations (IP). SDS-PAGE using 8% gels followed by Western blot analysis was performed. The membranes were probed with anti-phosphotyrosine ( alpha -pTyr) antibodies (PY20 and P99 mixture 1∶3,500) (top and t

<b>Biological Strategies Validation. </b>Migration of H1299 NSCLC cells enhanced by ligand-dependent Axl activation. (A) Western blotting to assess Gas6 expression in H1299 cells. Expression of Gas6 in LCAFhTERT cells was used as a positive control. (B) Phosphorylation of Axl was analyzed by Western blotting of whole cell lysates using different antibodies. GAPDH was used as an internal control. H1299 cells were stimulated for 15 min with 400 nM recombinant human Gas6 (rGas6). H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. (C) Migration of H1299 cells was analyzed using rGas6 (400 nM) added to the lower chamber. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. (D) Western blotting of conditioned medium from LCAFhTERT transfected with siGas6 or siScr (control) to assess whether they contains Gas6 secreted by CAFs. (E) Phosphorylation of Axl in H1299 cells analyzed by Western blotting after stimulation with conditioned medium from siRNA-transfected LCAFhTERT. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. The medium (DMEM containing 10% FBS) was used as control. (F) Migration of H1299 cells analyzed using conditioned medium of siRNA-transfected LCAFhTERT. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. The medium (DMEM containing 10% FBS) was used as control. The relative number of migrated H1299 cells is indicated on the y-axis. Data show the mean ± SEM (n = 3); **p < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28878389), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>HER2/HER3 and AXL expression and phosphorylation analysis.A) Representative western blots of total cell lysates of HCC827 and HCC4006 parental cell lines (P) and their derived ERL-resistant cell lines. Arrows indicate the expected molecular weight size. Total cell lysates loaded were 40 μg for AXL and pAXL analyses and 25 μg for the others. B) qPCR analysis of AXL mRNA normalized to rp-L31 mRNA and expressed relative to the levels in parental cell lines set as 1 (mean ± SD of triplicate determinations). Western blots and qPCR data are representative of those obtained respectively from 3 and 2 independent analysis. C) Dose-effect curves were calculated using CompuSyn software and plotting the entered Fa values against the entered dose values. For combination treatments, the combined drugs dose was entered. Each data point represents the mean of 3 replicates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0143333), licensed under a CC-BY license. Not internally tested by R&D Systems.

The expression of p-AXL&AXL positively correlates with the expression of KLF5 in human TNBC specimens. Moreover, DCC-2036 increases the sensitivity of TNBC chemotherapy by decreasing BCSCs.A Representative immunohistochemical staining images of p-AXL, AXL,&KLF5 protein in TNBC specimens, in which the expression of p-AXL, AXL,&KLF5 proteins was indicated by mild positive (+), moderate positive (++),&strong positive (+++), respectively. Left: Scale bars, 200 μm (magnification 40×); Right: Scale bars, 50 μm (magnification 100×). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36042208), licensed under a CC-BY license. Not internally tested by R&D Systems.

DCC-2036 inhibits CSCs in vivo according to the limited dilution assay by pretreating 4T1 cells with DCC-2036 or DMSO for 48 h.A In vivo tumorigenicity assay with limited dilution using DMSO or DCC-2036 treated 4T1 cells respectively: 200,000 (n = 7 and 8), 20,000 (n = 9 and 8), or 2000 (n = 10 and 10) cells per injection site. The frequency of BCSCs was calculated by ELDA. B Weight variation of mice following transplant of 4T1 cells into BALB/C mice after 7 days. C Images of tumor formation in BALB/C mice. D, E Transplanted tumors were harvested, and the tumor size and weight were measured at the end of the experiment. The statistical significance was determined by Student’s t-test. F Tumor-free survival curve of BALB/C mice is shown. G Hematoxylin and eosin (H&E) staining indicates the histology of tumor tissues. Scale bars, 20 μm. H Immunohistochemical analysis using p-AXL, AXL, and KLF5 antibodies in xenograft tissues from BALB/C mice. Scale bars, 20 μm. I Immunoblot of transplanted tumors from BALB/C mice posterior to the experiments. C means Control, T means DCC-2036. We chose the tumor tissues randomly for H&E staining, immunohistochemical analysis, and immunoblotting. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36042208), licensed under a CC-BY license. Not internally tested by R&D Systems.

Identification of Gas6/Axl-regulated transcripts in HCC cells. (A) Axl target genes were identified by RNA-seq of HCC cells stimulated with 500 ng/mL Gas6 for 24 h. p < 0.05 and an absolute log2-fold change of at least +1.0 were used for selection of targets. Axl dependencies were defined by comparisons of Axl-positive (wt, nick) versus Axl-negative (Axl−) cells (left panel). As indicated by the arrow, 298 genes were further intersected with differentially expressed genes of Axl-reconstituted cells (Axl−-wt). (B) Log2-fold changes (Log2FCs) of 8 Axl targets in Axl− vs. wt (yellow), Axl− vs. nick (blue) and Axl− vs. Axl−-wt (red). (C,D) Expression of PRAME transcript and protein levels as determined by qPCR and Western blotting, respectively. The expression of PRAME in Axl-expressing HCC cells was set to a value of 1. The expression of actin is shown as a loading control. (E) PRAME expression upon stimulation with 500 ng/mL Gas6 for 2 h in the absence (DMSO) or presence of 10 µM Axl inhibitor Cabozantinib (CABO) and upon administration of 900 ng/mL stimulatory Axl antibody (AF154) for 2 h. Protein levels were quantified by densitometry. wt, HLF; nick, HLF-nickase; Axl−, HLF-Axl-KO; Axl−-wt, HLF-Axl-KO-wt-Axl. Data are expressed as mean ± SD. ns: p > 0.05; *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37173882), licensed under a CC-BY license. Not internally tested by R&D Systems.

154-AL) was serially diluted and captured by Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Axl Fc Chimera (Catalog # <a class=

154-AL) was serially diluted and captured by Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF154U-100UG
Alexa Fluor 405	AF154V-100UG
Alexa Fluor 488	AF154G-100UG
Alexa Fluor 532	AF154X-100UG
Alexa Fluor 594	AF154T-100UG
Alexa Fluor 647	AF154R-100UG
Alexa Fluor 700	AF154N-100UG
Alexa Fluor 750	AF154S-100UG
Alexa Fluor Plus 405	AF154AFP405-100UG
Alexa Fluor Plus 488	AF154AFP488-100UG
Alexa Fluor Plus 555	AF154AFP555-100UG
Alexa Fluor Plus 594	AF154AFP594-100UG
Alexa Fluor Plus 647	AF154AFP647-100UG
Alexa Fluor Plus 680	AF154AFP680-100UG
Allophycocyanin	AF154A
Biotin	BAF154
CoraFluor 1	AF154CL1
DyLight 350	AF154D
DyLight 405	AF154E
DyLight 488	AF154K
DyLight 550	AF154L
DyLight 594	AF154M
DyLight 650	AF154W
DyLight 680	AF154Y
DyLight 755	AF154Z
FITC	AF154F
HRP	AF154H
Janelia Fluor 525	AF154JF525
Janelia Fluor 549	AF154I
Janelia Fluor 646	AF154J
Janelia Fluor 669	AF154JF669
PE	AF154P
PerCP	AF154C
mFluor Violet 450 SE	AF154MFV450
mFluor Violet 500 SE	AF154MFV500
mFluor Violet 610 SE	AF154MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF154-SP

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FAB154RAFP555

Axl Antibody (108724R) [Alexa Fluor™ Plus 555]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 555

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AF2228AFP594

Axl Antibody [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Alexa Fluor Plus 594

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FAB154AFP594

Axl Antibody (108724) [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 594

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AF154AFP680

Axl Antibody [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 680

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AF154AFP594

Axl Antibody [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 594

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AF154AFP405

Axl Antibody [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 405

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FAB8541AFP647

Axl Antibody (175128) [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor Plus 647

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FAB8541AFP680

Axl Antibody (175128) [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor Plus 680

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FAB854AFP647

Axl Antibody (107332) [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor Plus 647

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 558

Axl Recombinant Protein Antigen

Axl Recombinant Protein Antigen

Axl Antibody [Unconjugated]

Axl Antibody (108724R) [Alexa Fluor™ Plus 555]

Axl Antibody [Alexa Fluor™ Plus 594]

Axl Antibody (108724) [Alexa Fluor™ Plus 594]

Axl Antibody [Alexa Fluor™ Plus 680]

Axl Antibody [Alexa Fluor™ Plus 594]

Axl Antibody [Alexa Fluor™ Plus 405]

Axl Antibody (175128) [Alexa Fluor™ Plus 647]

Axl Antibody (175128) [Alexa Fluor™ Plus 680]

Axl Antibody (107332) [Alexa Fluor™ Plus 647]