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![Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TET2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TET2-Antibody-Western-Blot-NBP2-32104-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TET2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-32104] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TET2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TET2-Antibody-Immunohistochemistry-Paraffin-NBP2-32104-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TET2 antibody NBP2-32104 used for IP at 6 ug/mg lysate. TET2 was also immunoprecipitated by rabbit anti-TET2 antibody BL16766. For blotting immunoprecipitated TET2, NBP2-32104 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TET2-Antibody-Western-Blot-NBP2-32104-img0002.jpg)
![Immunohistochemistry-Paraffin: TET2 Antibody (CL6873) [NBP2-76510] - Analysis in human placenta and pancreas tissues. Corresponding TET2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Immunohistochemistry-Paraffin-NBP2-76510-img0002.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TET2 Antibody (CL6873) [NBP2-76510] - Analysis in human placenta and pancreas tissues. Corresponding TET2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: TET2 Antibody (CL6873) [NBP2-76510] - Analysis in human cell line HL-60.](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Western-Blot-NBP2-76510-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TET2 Antibody (CL6873) [NBP2-76510] - Staining of U-2 OS cells using the Anti-TET2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Immunocytochemistry-Immunofluorescence-NBP2-76510-img0006.jpg)
![Immunohistochemistry-Paraffin: TET2 Antibody (CL6873) [NBP2-76510] - Staining of human uterine cervix shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Immunohistochemistry-Paraffin-NBP2-76510-img0007.jpg)
![Immunohistochemistry-Paraffin: TET2 Antibody (CL6873) [NBP2-76510] - Staining of human placenta shows moderate nuclear positivity in a subset of trophoblastic cells.](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Immunohistochemistry-Paraffin-NBP2-76510-img0003.jpg)
![Immunohistochemistry-Paraffin: TET2 Antibody (CL6873) [NBP2-76510] - Staining of human tonsil shows weak to moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Immunohistochemistry-Paraffin-NBP2-76510-img0004.jpg)
![Immunohistochemistry-Paraffin: TET2 Antibody (CL6873) [NBP2-76510] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/TET2-Antibody-CL6873-Immunohistochemistry-Paraffin-NBP2-76510-img0005.jpg)
![Western Blot: TET2 Antibody (649) [NBP2-43552] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TET2-Antibody-649-Western-Blot-NBP2-43552-img0006.jpg "Western Blot: TET2 Antibody (649) [NBP2-43552] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Chromatin Immunoprecipitation: TET2 Antibody (649) [NBP2-43552] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.](http://images.novusbio.com/fullsize/TET2-Antibody-649-Chromatin-Immunoprecipitation-NBP2-43552-img0001.jpg)
![Immunoprecipitation: TET2 Antibody (649) [NBP2-43552] - TET2 antibody [GT649] immunoprecipitates TET2 protein in IP experiments.IP samples: 30 ug whole cell extract of TET2-transfected 293T cells.A. Control with 5 ug of preimmune Rabbit IgGB. Immunoprecipitation of TET2 protein by 5 ug TET2 antibody [GT649] (NBP2-43552)5 % SDS-PAGEThe immunoprecipitated TET2 protein was detected by TET2 antibody [GT649] (NBP2-43552) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-43552_mouse-monoclonal-tet2-antibody-649-91020242041263.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TET2 Antibody (649) [NBP2-43552] - Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [GT649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43552_mouse-monoclonal-tet2-antibody-649-1610202419405782.jpg)
![Immunohistochemistry: TET2 Antibody [NBP3-35520] - Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using TET2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35520_rabbit-tet2-pab-912202415143621.jpg "Immunohistochemistry: TET2 Antibody [NBP3-35520] - Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using TET2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: TET2 Antibody [NBP3-35520] - Western blot analysis of lysates from Mouse heart, using TET2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35520_rabbit-tet2-pab-91220241412380.jpg)
![Immunohistochemistry: TET2 Antibody [NBP3-35520] - Immunohistochemistry analysis of paraffin-embedded Human colon using TET2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35520_rabbit-tet2-pab-912202414154364.jpg)
![Western Blot: TET2 Antibody [NBP3-35741] - Western blot analysis of various lysates using TET2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35741_rabbit-tet2-pab-912202414213722.jpg "Western Blot: TET2 Antibody [NBP3-35741] - Western blot analysis of various lysates using TET2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Immunohistochemistry: TET2 Antibody [NBP3-35741] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TET2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35741_rabbit-tet2-pab-31120251744485.jpg)
![Immunohistochemistry: TET2 Antibody [NBP3-35741] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using TET2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35741_rabbit-tet2-pab-31120251704799.jpg)
![Immunohistochemistry: TET2 Antibody [NBP3-35741] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using TET2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35741_rabbit-tet2-pab-31120251704735.jpg)
![Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-27465] - Staining of paraffin embedded Human Spleen. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/TET2-Antibody-Immunohistochemistry-Paraffin-NBP2-27465-img0001.jpg "Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-27465] - Staining of paraffin embedded Human Spleen. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Flow Cytometry: TET2 Antibody [NBP2-27465] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TET2-Antibody-Flow-Cytometry-NBP2-27465-img0002.jpg)
![Western Blot: TET2 Antibody [NBP3-27879] - Western blot analysis of TET2 in SK-N-SH cell lysate with TET2 antibody at 1 u/mL.](http://images.novusbio.com/fullsize/nbp3-27879_rabbit-tet2-pab-2711202420272424.gif "Western Blot: TET2 Antibody [NBP3-27879] - Western blot analysis of TET2 in SK-N-SH cell lysate with TET2 antibody at 1 u/mL.")
![Immunocytochemistry/ Immunofluorescence: TET2 Antibody [NBP3-27879] - Immunofluorescence of TET2 in human colon carcinoma tissue with TET2 antibody at 20 u/ml.Green: TET2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp3-27879_rabbit-tet2-pab-2711202420243391.gif)
![Immunohistochemistry: TET2 Antibody [NBP3-27879] - Immunohistochemistry of TET2 in human colon carcinoma tissue with TET2 antibody at 2 u/ml.](http://images.novusbio.com/fullsize/nbp3-27879_rabbit-tet2-pab-271120242013116.gif)
![Western Blot: TET2 Antibody (C.15200179) [NBP2-59256] - Mouse E14 ES cells. The antibody was used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/TET2-Antibody-C-15200179-Western-Blot-NBP2-59256-img0004.jpg "Western Blot: TET2 Antibody (C.15200179) [NBP2-59256] - Mouse E14 ES cells. The antibody was used at a dilution of 1:1,000.")
![Western Blot: TET2 Antibody (C.15200179) [NBP2-59256] - Whole cell extracts from HL60 and NB4 cells (40 ug) were analysed by Western blot using the Diagenode antibody against TET2, diluted 1:2,000 (lane 1 and 2) or 1:1,000 (lane 3 and 4) in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/TET2-Antibody-C-15200179-Western-Blot-NBP2-59256-img0003.jpg)
![Immunoprecipitation: TET2 Antibody (C.15200179) [NBP2-59256] - IP was performed on 250 ug HL60 RIPA cell lysate using the antibody against TET2 (lane 3) or an IgG negative control (lane 2). The samples were analysed by Western blot. The input sample (25 ug RIPA lysate) was used as a positive control (lane 1).](http://images.novusbio.com/fullsize/TET2-Antibody-C-15200179-Western-Blot-NBP2-59256-img0001.jpg)
![Western Blot: TET2 Antibody (411) [NBP2-43682] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5 % SDS-PAGE TET2 antibody [411] dilution: 1:1000](http://images.novusbio.com/fullsize/TET2-Antibody-411-Western-Blot-NBP2-43682-img0002.jpg "Western Blot: TET2 Antibody (411) [NBP2-43682] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5 % SDS-PAGE TET2 antibody [411] dilution: 1:1000")
![Immunoprecipitation: TET2 Antibody (411) [NBP2-43682] - Analysis of 30 ug whole cell extract of TET2-transfected 293T cells. A. Control with 5 ug of preimmune Rabbit IgG B. Immunoprecipitation of TET2 protein by 5 ug TET2 antibody [411] 5 % SDS-PAGE The immunoprecipitated TET2 protein was detected by TET2 antibody [411] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent] .](http://images.novusbio.com/fullsize/TET2-Antibody-411-Immunoprecipitation-NBP2-43682-img0001.jpg)
![Western Blot: TET2 Antibody (442) [NBP2-43579] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5 % SDS-PAGE TET2 antibody [442] dilution: 1:5000](http://images.novusbio.com/fullsize/TET2-Antibody-442-Western-Blot-NBP2-43579-img0002.jpg "Western Blot: TET2 Antibody (442) [NBP2-43579] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5 % SDS-PAGE TET2 antibody [442] dilution: 1:5000")
![Immunoprecipitation: TET2 Antibody (442) [NBP2-43579] - Analysis of 30 ug whole cell extract of TET2-transfected 293T cells. A. Control with 5 ug of preimmune Rabbit IgG B. Immunoprecipitation of TET2 protein by 5 ug TET2 antibody [422] 5 % SDS-PAGE The immunoprecipitated TET2 protein was detected by TET2 antibody [422] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent] .](http://images.novusbio.com/fullsize/TET2-Antibody-442-Immunoprecipitation-NBP2-43579-img0001.jpg)
![Western Blot: TET2 Antibody [NBP3-27891] - Western blot analysis of TET2 in 3T3 cell lysate with TET2 antibody at 1 u/ml.](http://images.novusbio.com/fullsize/nbp3-27891_rabbit-tet2-pab-271120241929383.gif "Western Blot: TET2 Antibody [NBP3-27891] - Western blot analysis of TET2 in 3T3 cell lysate with TET2 antibody at 1 u/ml.")