HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5." alt="Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3417) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3417-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87166-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87165-25ul | |||
![Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human PolH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody NB100-60424 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Western-Blot-NB100-60424-img0005.jpg "Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human PolH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody NB100-60424 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human XPV/DNA polymerase eta/PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PolH antibody NB100-60424 (lot 3) used for IP at 6 ug per reaction. PolH was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-PolH antibody NB100-60423. For blotting immunoprecipitated PolH, NB100-60424 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Immunoprecipitation-NB100-60424-img0006.jpg)
![Western Blot: XPV/DNA polymerase eta Antibody [NBP1-87166] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Western-Blot-NBP1-87166-img0004.jpg "Western Blot: XPV/DNA polymerase eta Antibody [NBP1-87166] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: XPV/DNA polymerase eta Antibody [NBP1-87166] - Staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Immunohistochemistry-Paraffin-NBP1-87166-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: XPV/DNA polymerase eta Antibody [NBP1-87165] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87165-img0005.jpg "Immunocytochemistry/Immunofluorescence: XPV/DNA polymerase eta Antibody [NBP1-87165] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: XPV/DNA polymerase eta Antibody [NBP1-87165] - Staining of human lymph node shows moderate cytoplasmic and nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Immunohistochemistry-Paraffin-NBP1-87165-img0006.jpg)
![Western Blot: DNA polymerase eta Overexpression Lysate (Adult Normal) [NBP2-10408] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA polymerase eta.](http://images.novusbio.com/fullsize/DNA-polymerase-eta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10408-img0001.jpg "Western Blot: DNA polymerase eta Overexpression Lysate (Adult Normal) [NBP2-10408] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA polymerase eta.")
![Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60423] - Detection of Human PolH by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolH antibody NB100-60423 used for IP at 3 mcg/mg lysate. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60424, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/XPV-DNA-polymerase-eta-Antibody-Immunoprecipitation-NB100-60423-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60423] - Detection of Human PolH by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolH antibody NB100-60423 used for IP at 3 mcg/mg lysate. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60424, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.")
