PLC-gamma 1: Novus Biologicals

NBP2-52533

PLC-gamma 1 Antibody (3H1C10) - BSA Free

(1 Publication)

0.1 mg

NBP2-52533

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of paraffin-embedded esophagus tissues using PLCG1 mouse mAb with DAB staining.

Flow Cytometry: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of Raji cells using PLCG1 mouse mAb (green) and negative control (red).

Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Analysis of paraffin-embedded breast cancer tissues using PLCG1 mouse mAb with DAB staining.

ELISA: PLC-gamma 1 Antibody (3H1C10) [NBP2-52533] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52533-0.025mg

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NBP2-52532

PLC-gamma 1 Antibody (3C2D11) - BSA Free

0.1 mg

NBP2-52532

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded bladder cancer tissues using PLCG1 mouse mAb with DAB staining.

Flow Cytometry: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of Jurkat cells using PLCG1 mouse mAb (green) and negative control (red).

Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Western blot analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)

Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded cervical cancer tissues using PLCG1 mouse mAb with DAB staining.

ELISA: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Reactivity:

Hu, Rt, Pm

Species Glossary

Reactivity: Hu, Rt, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52532-0.025mg

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AF3288

PLC-gamma 1 Antibody [Unconjugated]

(1 Publication)

100 μg

AF3288

HAF017). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PLC- gamma1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PLC- gamma1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Left:</b> Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.1 mg/ml. A specific band was detected for PLC‑ gamma 1 at approximately 150 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Goat Anti-Human/Mouse/Rat PLC‑ gamma 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. <b>Right:</b> Simple Western electropherogram showing the same Goat Anti-Human/Mouse/Rat PLC‑ gamma 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the Jurkat human acute T cell leukemia cell line.

TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein (~2mg) were co-immunoprecipitated with pAb anti-TIM-3 antibody and western blot was performed using capillary electrophoresis. Cleared lysate served as a loading control for individual antibody reactivity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140694), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3288U-100UG
Alexa Fluor 405	AF3288V-100UG
Alexa Fluor 488	AF3288G-100UG
Alexa Fluor 532	AF3288X-100UG
Alexa Fluor 594	AF3288T-100UG
Alexa Fluor 647	AF3288R-100UG
Alexa Fluor 700	AF3288N-100UG
Alexa Fluor 750	AF3288S-100UG
Alexa Fluor Plus 405	AF3288AFP405-100UG
Alexa Fluor Plus 488	AF3288AFP488-100UG
Alexa Fluor Plus 555	AF3288AFP555-100UG
Alexa Fluor Plus 594	AF3288AFP594-100UG
Alexa Fluor Plus 647	AF3288AFP647-100UG
Alexa Fluor Plus 680	AF3288AFP680-100UG
Allophycocyanin	AF3288A
Biotin	AF3288B
CoraFluor 1	AF3288CL1
DyLight 350	AF3288D
DyLight 405	AF3288E
DyLight 488	AF3288K
DyLight 550	AF3288L
DyLight 594	AF3288M
DyLight 650	AF3288W
DyLight 680	AF3288Y
DyLight 755	AF3288Z
FITC	AF3288F
HRP	AF3288H
Janelia Fluor 525	AF3288JF525
Janelia Fluor 549	AF3288I
Janelia Fluor 646	AF3288J
Janelia Fluor 669	AF3288JF669
PE	AF3288P
PerCP	AF3288C
mFluor Violet 450 SE	AF3288MFV450
mFluor Violet 500 SE	AF3288MFV500
mFluor Violet 610 SE	AF3288MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3288-SP

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H00005335-M01

PLC-gamma 1 Antibody (2A2) - Azide and BSA Free

(2 Publications)

0.1 mg

H00005335-M01

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between PTK2 and PLCG1. HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between HCK and PLCG1. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Analysis of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: PLC-gamma 1 Antibody (2A2) [H00005335-M01] - Detection limit for recombinant GST tagged PLCG1 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB74542

PLC-gamma 1 [p Tyr783] Antibody (1016D) [Unconjugated]

(2 Publications)

100 μg

MAB74542

236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>PLC- gamma1 phosphorylated at Y783 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # <a class=

236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PLC- gamma1 phosphorylated at Y783 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

<b>Biological Strategies Validation. </b>Cathelicidin-dependent signaling in platelets. a–i LL-37 induced signaling in isolated human platelets. a–c Flow cytometry analysis of platelet P-selectin surface expression in the presence of a the calcium chelator BAPTA (n = 5) or a phospholipase C inhibitor (U-73122, n = 6), b pertussis or cholera toxin to inhibit G-protein signaling (n = 4), or c inhibitors of tyrosine kinases Src-family kinases (Dasatinib, n = 7) and Syk (R406, n = 5). d Representative western blots of phosphorylated Src-family kinase and phosphorylated Syk upon incubation of platelets with LL-37. Collagen was used as positive control for tyrosine kinase phosphorylation, beta -actin served as loading control. Images are representative of three independent blots. e–i Flow cytometry analysis of LL-37 platelet P-selectin surface expression in the presence of e STAT3 small molecule inhibitor (Stattic, n = 6), f GPVI antibody (HGP5C4, n = 9), g GPIIb/IIIa antibody (Abciximab or Tirofiban, n = 4), h formyl-peptid-receptor (FPR1 or FPR2) antibody, and i inhibitors against the purinergic P2X7-receptor (Boc-MLF 10, WRW4 or A438079, n = 4). j–l CRAMP-induced signaling in isolated mouse platelets. j P-selectin surface expression in the presence of a GPVI depleting antibody (JAQ1, n = 6). k P-selectin surface expression and l phospholipase C phosphorylation in platelet lysates from platelet-specific Syk-deficient mice and respective littermates after stimulation with CRAMP (flow cytometry: n = 10 Syk−/− and n = 5 Syk+/+ animals, western blot analysis n = 5 each). Graphs show mean and SEM. P-values were determined by unpaired (a, f, j–l) or paired (c, e, h) t-test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29670076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB74542U-100UG
Alexa Fluor 405	FAB74542V-100UG
Alexa Fluor 488	FAB74542G-100UG
Alexa Fluor 532	FAB74542X-100UG
Alexa Fluor 594	FAB74542T-100UG
Alexa Fluor 647	FAB74542R-100UG
Alexa Fluor 700	FAB74542N-100UG
Alexa Fluor 750	FAB74542S-100UG
Alexa Fluor Plus 405	FAB74542AFP405-100UG
Alexa Fluor Plus 488	FAB74542AFP488-100UG
Alexa Fluor Plus 555	FAB74542AFP555-100UG
Alexa Fluor Plus 594	FAB74542AFP594-100UG
Alexa Fluor Plus 647	FAB74542AFP647-100UG
Alexa Fluor Plus 680	FAB74542AFP680-100UG
Allophycocyanin	FAB74542A
Biotin	FAB74542B
CoraFluor 1	FAB74542CL1
DyLight 350	FAB74542D
DyLight 405	FAB74542E
DyLight 488	FAB74542K
DyLight 550	FAB74542L
DyLight 594	FAB74542M
DyLight 650	FAB74542W
DyLight 680	FAB74542Y
DyLight 755	FAB74542Z
FITC	FAB74542F
HRP	FAB74542H
Janelia Fluor 525	FAB74542JF525
Janelia Fluor 549	FAB74542I
Janelia Fluor 646	FAB74542J
Janelia Fluor 669	FAB74542JF669
PE	FAB74542P
PerCP	FAB74542C
mFluor Violet 450 SE	FAB74542MFV450
mFluor Violet 500 SE	FAB74542MFV500
mFluor Violet 610 SE	FAB74542MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB74542-SP

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MAB74541

PLC-gamma 1 [p Tyr783] Antibody (1016A) [Unconjugated]

(1 Publication)

100 μg

MAB74541

HAF008). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PLC- gamma1 (Y783) Monoclonal Antibody (Catalog # MAB74541) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PLC- gamma1 (Y783) Monoclonal Antibody (Catalog # MAB74541) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB74541U-100UG
Alexa Fluor 405	FAB74541V-100UG
Alexa Fluor 488	FAB74541G-100UG
Alexa Fluor 532	FAB74541X-100UG
Alexa Fluor 594	FAB74541T-100UG
Alexa Fluor 647	FAB74541R-100UG
Alexa Fluor 700	FAB74541N-100UG
Alexa Fluor 750	FAB74541S-100UG
Alexa Fluor Plus 405	FAB74541AFP405-100UG
Alexa Fluor Plus 488	FAB74541AFP488-100UG
Alexa Fluor Plus 555	FAB74541AFP555-100UG
Alexa Fluor Plus 594	FAB74541AFP594-100UG
Alexa Fluor Plus 647	FAB74541AFP647-100UG
Alexa Fluor Plus 680	FAB74541AFP680-100UG
Allophycocyanin	FAB74541A
Biotin	FAB74541B
CoraFluor 1	FAB74541CL1
DyLight 350	FAB74541D
DyLight 405	FAB74541E
DyLight 488	FAB74541K
DyLight 550	FAB74541L
DyLight 594	FAB74541M
DyLight 650	FAB74541W
DyLight 680	FAB74541Y
DyLight 755	FAB74541Z
FITC	FAB74541F
HRP	FAB74541H
Janelia Fluor 525	FAB74541JF525
Janelia Fluor 549	FAB74541I
Janelia Fluor 646	FAB74541J
Janelia Fluor 669	FAB74541JF669
PE	FAB74541P
PerCP	FAB74541C
mFluor Violet 450 SE	FAB74541MFV450
mFluor Violet 500 SE	FAB74541MFV500
mFluor Violet 610 SE	FAB74541MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB74541-SP

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MAB32881

PLC-gamma 1 Antibody (479819)

100 μg

MAB32881

HAF005). A specific band was detected for PLC- gamma1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC- gamma1 Monoclonal Antibody (Catalog # MAB32881) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for PLC- gamma1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC- gamma1 Monoclonal Antibody (Catalog # MAB32881) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLC- gamma1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB32881U-100UG
Alexa Fluor 405	FAB32881V-100UG
Alexa Fluor 488	FAB32881G-100UG
Alexa Fluor 532	FAB32881X-100UG
Alexa Fluor 594	FAB32881T-100UG
Alexa Fluor 647	FAB32881R-100UG
Alexa Fluor 700	FAB32881N-100UG
Alexa Fluor 750	FAB32881S-100UG
Alexa Fluor Plus 405	FAB32881AFP405-100UG
Alexa Fluor Plus 488	FAB32881AFP488-100UG
Alexa Fluor Plus 555	FAB32881AFP555-100UG
Alexa Fluor Plus 594	FAB32881AFP594-100UG
Alexa Fluor Plus 647	FAB32881AFP647-100UG
Alexa Fluor Plus 680	FAB32881AFP680-100UG
Allophycocyanin	FAB32881A
Biotin	FAB32881B
CoraFluor 1	FAB32881CL1
DyLight 350	FAB32881D
DyLight 405	FAB32881E
DyLight 488	FAB32881K
DyLight 550	FAB32881L
DyLight 594	FAB32881M
DyLight 650	FAB32881W
DyLight 680	FAB32881Y
DyLight 755	FAB32881Z
FITC	FAB32881F
HRP	FAB32881H
Janelia Fluor 525	FAB32881JF525
Janelia Fluor 549	FAB32881I
Janelia Fluor 646	FAB32881J
Janelia Fluor 669	FAB32881JF669
PE	FAB32881P
PerCP	FAB32881C
mFluor Violet 450 SE	FAB32881MFV450
mFluor Violet 500 SE	FAB32881MFV500
mFluor Violet 610 SE	FAB32881MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB32881-SP

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MAB7454

PLC-gamma 1 [p Tyr783] Antibody (764518) [Unconjugated]

100 μg

MAB7454

HAF018). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF018). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-PLC- gamma1 (Y783) Monoclonal Antibody (Catalog # MAB7454) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7454U-100UG
Alexa Fluor 405	FAB7454V-100UG
Alexa Fluor 488	FAB7454G-100UG
Alexa Fluor 532	FAB7454X-100UG
Alexa Fluor 594	FAB7454T-100UG
Alexa Fluor 647	FAB7454R-100UG
Alexa Fluor 700	FAB7454N-100UG
Alexa Fluor 750	FAB7454S-100UG
Alexa Fluor Plus 405	FAB7454AFP405-100UG
Alexa Fluor Plus 488	FAB7454AFP488-100UG
Alexa Fluor Plus 555	FAB7454AFP555-100UG
Alexa Fluor Plus 594	FAB7454AFP594-100UG
Alexa Fluor Plus 647	FAB7454AFP647-100UG
Alexa Fluor Plus 680	FAB7454AFP680-100UG
Allophycocyanin	FAB7454A
Biotin	FAB7454B
CoraFluor 1	FAB7454CL1
DyLight 350	FAB7454D
DyLight 405	FAB7454E
DyLight 488	FAB7454K
DyLight 550	FAB7454L
DyLight 594	FAB7454M
DyLight 650	FAB7454W
DyLight 680	FAB7454Y
DyLight 755	FAB7454Z
FITC	FAB7454F
HRP	FAB7454H
Janelia Fluor 525	FAB7454JF525
Janelia Fluor 549	FAB7454I
Janelia Fluor 646	FAB7454J
Janelia Fluor 669	FAB7454JF669
PE	FAB7454P
PerCP	FAB7454C
mFluor Violet 450 SE	FAB7454MFV450
mFluor Violet 500 SE	FAB7454MFV500
mFluor Violet 610 SE	FAB7454MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7454-SP

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NBP2-38317

PLC-gamma 1 Antibody - BSA Free

0.1 ml

NBP2-38317

Immunohistochemistry: PLC-gamma 1 Antibody [NBP2-38317] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-38317-25ul

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NBP2-61790

PLC-gamma 1 Antibody (2F3D11) - BSA Free

0.1 ml

NBP2-61790

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mouse mAb against Jurkat (1), K562 (2), A431 (3), Hela (4), and PC-12 (5) cell lysate.

ELISA: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis of Hela cells using PLCG1 mouse mAb (green) and negative control (red).

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-61790-0.025ml

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MAB3288

PLC-gamma 1 Antibody (346130)

100 μg

MAB3288

HAF007). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HEK293 human embryonic kidney cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-gamma 1 Monoclonal Antibody (Catalog # MAB3288) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-gamma 1 Monoclonal Antibody (Catalog # MAB3288) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3288U-100UG
Alexa Fluor 405	FAB3288V-100UG
Alexa Fluor 488	FAB3288G-100UG
Alexa Fluor 532	FAB3288X-100UG
Alexa Fluor 594	FAB3288T-100UG
Alexa Fluor 647	FAB3288R-100UG
Alexa Fluor 700	FAB3288N-100UG
Alexa Fluor 750	FAB3288S-100UG
Alexa Fluor Plus 405	FAB3288AFP405-100UG
Alexa Fluor Plus 488	FAB3288AFP488-100UG
Alexa Fluor Plus 555	FAB3288AFP555-100UG
Alexa Fluor Plus 594	FAB3288AFP594-100UG
Alexa Fluor Plus 647	FAB3288AFP647-100UG
Alexa Fluor Plus 680	FAB3288AFP680-100UG
Allophycocyanin	FAB3288A
Biotin	FAB3288B
CoraFluor 1	FAB3288CL1
DyLight 350	FAB3288D
DyLight 405	FAB3288E
DyLight 488	FAB3288K
DyLight 550	FAB3288L
DyLight 594	FAB3288M
DyLight 650	FAB3288W
DyLight 680	FAB3288Y
DyLight 755	FAB3288Z
FITC	FAB3288F
HRP	FAB3288H
Janelia Fluor 525	FAB3288JF525
Janelia Fluor 549	FAB3288I
Janelia Fluor 646	FAB3288J
Janelia Fluor 669	FAB3288JF669
PE	FAB3288P
PerCP	FAB3288C
mFluor Violet 450 SE	FAB3288MFV450
mFluor Violet 500 SE	FAB3288MFV500
mFluor Violet 610 SE	FAB3288MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3288-SP

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NBP2-38316

PLC-gamma 1 Antibody - BSA Free

0.1 ml

NBP2-38316

Staining of human cell line U-2 OS shows localization to cytosol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-38316-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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