| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91810-25ul | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CRBN was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human CRBN Polyclonal Antibody (Catalog # MAB9574) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9574-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92446-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91810-img0027.jpg "Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.")
![Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP1-91810-img0025.jpg)
![Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CRBN-Antibody-Simple-Western-NBP1-91810-img0014.jpg)
![Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/CRBN-Antibody-Simple-Western-NBP1-91810-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Single Cell Western: CRBN Antibody [NBP1-91810] - CRBN expression after Downregulation by siRNA, dilution 1:500. Single Cell Western Image submitted by verified customer review.](http://images.novusbio.com/fullsize/CRBN-Antibody-Single-Cell-Western-NBP1-91810-img0035.jpg)
![Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202420243341.jpg "Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![Western Blot: CRBN Antibody [NBP2-82079] - Analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP2-82079-img0001.jpg)
![Immunohistochemistry: CRBN Antibody [NBP2-82079] - Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunohistochemistry-NBP2-82079-img0002.jpg)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Human Cells. Loading: 15 ug of cell lysate Antibodies: CRBN, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-271120242013238.gif)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Mouse Tissues.Loading: 15 ug of mouse tissue lysate Antibodies: CRBN, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202420141976.gif)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Rat Tissues. Loading: 15 ug of rat tissue lysate Antibodies: CRBN, 0.5 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202419592026.gif)
![Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP2-82079] - Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82079-img0003.jpg)
![Western Blot: CRBN Antibody [H00051185-B01P] - Analysis of CRBN expression in transfected 293T cell line by CRBN polyclonal antibody. Lane 1: CRBN transfected lysate(48.62 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-H00051185-B01P-img0002.jpg "Western Blot: CRBN Antibody [H00051185-B01P] - Analysis of CRBN expression in transfected 293T cell line by CRBN polyclonal antibody. Lane 1: CRBN transfected lysate(48.62 KDa). Lane 2: Non-transfected lysate.")
![SDS-Page: Recombinant Human CRBN Protein [H00051185-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-CRBN-Protein-SDS-Page-H00051185-P01-img0002.jpg "SDS-Page: Recombinant Human CRBN Protein [H00051185-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for ![Western Blot: CRBN Antibody [NBP2-92446] - Western blot analysis of extracts of various cell lines, using CRBN antibody (NBP2-92446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CRBN Antibody-Western Blot-NBP2-92446-img0001.jpg "Western Blot: CRBN Antibody [NBP2-92446] - Western blot analysis of extracts of various cell lines, using CRBN antibody (NBP2-92446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry: CRBN Antibody (PSH0-70) [NBP3-32217] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (NBP3-32217) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32217_rabbit-crbn-mab-psh0-70-298202413585562.jpg "Immunohistochemistry: CRBN Antibody (PSH0-70) [NBP3-32217] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (NBP3-32217) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: CRBN Antibody [NBP2-87207] - Host: Rabbit. Target Name: CRBN. Sample Type: Jurkat Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP2-87207-img0001.jpg "Western Blot: CRBN Antibody [NBP2-87207] - Host: Rabbit. Target Name: CRBN. Sample Type: Jurkat Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: CRBN Overexpression Lysate (Adult Normal) [NBP2-07061] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRBN.](http://images.novusbio.com/fullsize/CRBN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07061-img0001.jpg "Western Blot: CRBN Overexpression Lysate (Adult Normal) [NBP2-07061] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRBN.")
![ELISA: Human CRBN - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31215] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31215_human-crbn-ready-to-use-elisa-kit-colorimetric-206202415322775.png "ELISA: Human CRBN - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31215] - Standard Curve Reference")
