| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92853-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12686-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92611-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56452-25ul | |||
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249413328.jpg "Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Genetic Strategies Validation. </b>Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249425474.jpg)
![Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024942544.jpg)
![Western Blot: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-21202494142.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-2120249432896.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024938315.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody (HL1909) - Azide and BSA Free [NBP3-25371] - Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Ataxin 3 stained by Ataxin 3 antibody [HL1909] (NBP3-25371) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25371_rabbit-ataxin-3-mab-hl1909-azide-and-bsa-free-212024937492.jpg)
![Western Blot: Ataxin-3 Antibody [NBP2-92853] - Analysis of extracts of various cell lines, using Ataxin-3 antibody (NBP2-92853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Ataxin-3 Antibody-Western Blot-NBP2-92853-img0007.jpg "Western Blot: Ataxin-3 Antibody [NBP2-92853] - Analysis of extracts of various cell lines, using Ataxin-3 antibody (NBP2-92853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-92853] - Immunofluorescence analysis of U2OS cells using Ataxin-3 Rabbit pAb (NBP2-92853) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92853-img0003.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP2-92853] - Rat kidney using Ataxin-3 Rabbit pAb (NBP2-92853) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-92853-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-92853] - Analysis of U-2 OS cells using Ataxin-3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92853-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-92853] - Immunofluorescence analysis of HeLa cells using Ataxin-3 Rabbit pAb (NBP2-92853) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92853-img0002.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP2-92853] - Human esophageal cancer using Ataxin-3 Rabbit pAb (NBP2-92853) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-92853-img0004.jpg)
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP2-92853] - Mouse lung using Ataxin-3 Rabbit pAb (NBP2-92853) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-92853-img0005.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0011.jpg "Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0008.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0006.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3. 10 % SDS-PAGE Ataxin 3 antibody dilution: 1:20000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0007.jpg)
![Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP3-12686-img0001.jpg "Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa")
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP3-12686] - Staining of human ATXN3 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP3-12686-img0002.jpg)
![Immunoprecipitation: Ataxin-3 Antibody [NBP3-12686] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-ATXN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATXN3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size :50 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunoprecipitation-NBP3-12686-img0003.jpg)
![Western Blot: Ataxin-3 Antibody [NBP2-92611] - Analysis of extracts of various cell lines, using Ataxin-3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP2-92611-img0002.jpg "Western Blot: Ataxin-3 Antibody [NBP2-92611] - Analysis of extracts of various cell lines, using Ataxin-3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-92611] - Analysis of HeLa cells using Ataxin-3 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92611-img0001.jpg)
![Western Blot: Ataxin-3 Antibody [NBP2-87047] - Lanes: Lane 1: 2ug hATX3 (isoform2); Josephin domain(1-182). Lane 2: 2ug hATX3 (isoform2); C-terminal truncated Atx3 (1-264). Lane 3: 2ug hATX3 (isoform2). Lane 4: 2ug other protein. Primary Antibody Dilution: 1:10,000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:20,000. Gene Name: ATXN3. Submitted by: Dr. Maria Macedo, Instituto de Biologia Molecular e Celular.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP2-87047-img0002.jpg "Western Blot: Ataxin-3 Antibody [NBP2-87047] - Lanes: Lane 1: 2ug hATX3 (isoform2); Josephin domain(1-182). Lane 2: 2ug hATX3 (isoform2); C-terminal truncated Atx3 (1-264). Lane 3: 2ug hATX3 (isoform2). Lane 4: 2ug other protein. Primary Antibody Dilution: 1:10,000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:20,000. Gene Name: ATXN3. Submitted by: Dr. Maria Macedo, Instituto de Biologia Molecular e Celular.")
![Western Blot: Ataxin-3 Antibody [NBP2-87047] - WB Suggested Anti-ATXN3 Antibody Titration: 0.2-1 ug/ml. Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP2-87047-img0001.jpg)
![Western Blot: Ataxin-3 Antibody (S04-1I8) [NBP3-19834] - Western blot detection of Ataxin-3 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19834 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-S04-1I8-Western-Blot-NBP3-19834-img0001.jpg "Western Blot: Ataxin-3 Antibody (S04-1I8) [NBP3-19834] - Western blot detection of Ataxin-3 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19834 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.")
![Western Blot: Ataxin-3 Antibody (7B2I1) [NBP3-33328] - Western blot analysis of lysates from various lysates, using Ataxin-3Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33328_rabbit-ataxin-3-mab-7b2i1-1012202411545530.jpg "Western Blot: Ataxin-3 Antibody (7B2I1) [NBP3-33328] - Western blot analysis of lysates from various lysates, using Ataxin-3Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-56452] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56452-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-56452] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![SDS-Page: Recombinant Human Ataxin-3 Protein [NBP1-50828] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Ataxin-3-Protein-SDS-Page-NBP1-50828-img0001.jpg "SDS-Page: Recombinant Human Ataxin-3 Protein [NBP1-50828] - 15 % SDS-PAGE (3ug)")