MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">
MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Jak3 Monoclonal Antibody (Catalog # MAB46991, filled histogram) or isotype control antibody (Catalog #
MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Jak3 Monoclonal Antibody (Catalog # MAB46991, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
![Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).](http://images.novusbio.com/fullsize/Jak3-Antibody-5H2-Western-Blot-NBP2-37737-img0001.jpg "Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).")
![Immunocytochemistry/Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Jak3-Antibody-5H2-Immunocytochemistry-Immunofluorescence-NBP2-37737-img0003.jpg)
![Flow Cytometry: Jak3 Antibody (5H2) [NBP2-37737] - Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Jak3-Antibody-5H2-Flow-Cytometry-NBP2-37737-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Jak3-Antibody-5H2-Immunocytochemistry-Immunofluorescence-NBP2-37737-img0002.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for ![Immunohistochemistry-Paraffin: Jak3 Antibody [NBP2-49629] - Staining in human appendix and liver tissues using anti-JAK3 antibody. Corresponding JAK3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Jak3-Antibody-Immunohistochemistry-Paraffin-NBP2-49629-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak3 Antibody [NBP2-49629] - Staining in human appendix and liver tissues using anti-JAK3 antibody. Corresponding JAK3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Jak3 Antibody [NBP2-49629] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/Jak3-Antibody-Immunohistochemistry-Paraffin-NBP2-49629-img0003.jpg)
![Immunohistochemistry-Paraffin: Jak3 Antibody [NBP2-49629] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Jak3-Antibody-Immunohistochemistry-Paraffin-NBP2-49629-img0004.jpg)
![Western Blot: JAK3 Overexpression Lysate (Adult Normal) [NBL1-12094] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JAK3.](http://images.novusbio.com/fullsize/JAK3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12094-img0002.jpg "Western Blot: JAK3 Overexpression Lysate (Adult Normal) [NBL1-12094] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JAK3.")
![Western Blot: Jak3 Antibody [NBP3-12702] - Lane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:125 kDaObserved band size:130 kDa](http://images.novusbio.com/fullsize/Jak3-Antibody-Western-Blot-NBP3-12702-img0001.jpg "Western Blot: Jak3 Antibody [NBP3-12702] - Lane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:125 kDaObserved band size:130 kDa")
![Immunohistochemistry: Jak3 Antibody (1C7) [NBP3-15196] - IHC image of JAK3 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Jak3-Antibody-1C7-Immunohistochemistry-NBP3-15196-img0001.jpg "Immunohistochemistry: Jak3 Antibody (1C7) [NBP3-15196] - IHC image of JAK3 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![ELISA: Human Jak3 ELISA Kit (Colorimetric) [NBP3-39643] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39643_human-jak3-elisa-kit-colorimetric-211020241528237.png "ELISA: Human Jak3 ELISA Kit (Colorimetric) [NBP3-39643] - Standard Curve Reference")
![ELISA: Human Jak3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39644] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39644_human-jak3-ready-to-use-elisa-kit-colorimetric-211020241540312.png "ELISA: Human Jak3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39644] - Standard Curve Reference")
