Products Related to Gene-ID: 3502

MAB1435, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB1435, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG₃(Catalog # MAB9675) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific bands was detected for IgG₃isotype antibody at approximately 26 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018) but not Rabbit Anti-Mouse IgG_2BMonoclonal Antibody (Catalog # MAB9675). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG₃(Catalog # MAB9675) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific bands was detected for IgG₃isotype antibody at approximately 26 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018) but not Rabbit Anti-Mouse IgG_2BMonoclonal Antibody (Catalog # MAB9675). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG₃(Catalog # MAB9675) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific bands was detected for IgG₃isotype antibody at approximately 26 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018) but not Rabbit Anti-Mouse IgG_2BMonoclonal Antibody (Catalog # MAB9675). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB9186, Mouse IgG₃isotype), followed by Rabbit anti-Mouse IgG₃-specific secondary antibody (Catalog # MAB9675, filled histogram), Rabbit anti-Mouse IgG₁-specific (Catalog # MAB9627, blue line), Rabit anti-Mouse IgG_2A-specific (Catalog # MAB0033, red line), or Rabbit anti-Mouse IgG_2B-specific (Catalog # MAB9636, black line) secondary antibody, then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB9186, Mouse IgG₃isotype), followed by Rabbit anti-Mouse IgG₃-specific secondary antibody (Catalog # MAB9675, filled histogram), Rabbit anti-Mouse IgG₁-specific (Catalog # MAB9627, blue line), Rabit anti-Mouse IgG_2A-specific (Catalog # MAB0033, red line), or Rabbit anti-Mouse IgG_2B-specific (Catalog # MAB9636, black line) secondary antibody, then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

HAF018). A specific band was detected for IgG₃at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IgG₃at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of purified human IgG₁(negative control), purified human IgG₂(negative control), purified human IgG₃, and human purified human IgG₄(negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG₃Monoclonal Antibody (Catalog # MAB10122) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG₃at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />