MAB9894) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB9894) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Human IgG
1protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG
1Monoclonal Antibody (Catalog #
MAB9894) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB9794) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB9794) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB9895) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB9895) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB11013) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog #MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB11013) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog #MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB5994, filled histogram) or isotype control antibody (Catalog # MAB006), open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) at 10 µL/106cells." alt="bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994, filled histogram) or isotype control antibody (Catalog # MAB006), open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) at 10 µL/106cells." class="big_thumb" />
FAB4867A) and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />
MAB1139, filled histogram) or mouse IgG isotype control antibody (open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114) at 10 uL/106cells." alt="Human peripheral blood granuloctyes were stained with Mouse Anti-Human Siglec-9 Monoclonal Antibody (Catalog # MAB1139, filled histogram) or mouse IgG isotype control antibody (open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114) at 10 uL/106cells." class="big_thumb" />
MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human IgG1protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG1Monoclonal Antibody (Catalog # MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![ELISA: Goat IgG ELISA Kit (Colorimetric) [NBP2-60061] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Goat-IgG-ELISA-Kit-Colorimetric-ELISA-NBP2-60061-img0001.jpg "ELISA: Goat IgG ELISA Kit (Colorimetric) [NBP2-60061] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![Goat IgG ELISA Kit (Colorimetric) [NBP2-60061] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-60061_goat-igg-elisa-kit-colorimetric-3120248504645.png)
![SDS-Page: Sheep IgG Isotype Control [NBP1-97055] - Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1.0 ug per lane Non-Reduced Predicted/Observed Size: 160 kDa/160 kDa Reduced Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa](http://images.novusbio.com/fullsize/Sheep IgG Isotype Control-SDS-Page-NBP1-97055-img0005.jpg "SDS-Page: Sheep IgG Isotype Control [NBP1-97055] - Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1.0 ug per lane Non-Reduced Predicted/Observed Size: 160 kDa/160 kDa Reduced Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa")
![SDS-Page: Sheep IgG Isotype Control [NBP1-97055] - SDS PAGE Results of Sheep IgG Isotype control. Lane 1: Sheep IgG Reduced [5ug]. Lane 2: Sheep IgG Reduced [1ug]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Sheep IgG Non-Reduced [1ug]. Lane 5: Sheep IgG Non-Reduced [5ug]. 4-20% Gel, Coomassie stained.](http://images.novusbio.com/fullsize/Sheep-IgG-Isotype-Control-SDS-Page-NBP1-97055-img0004.jpg)
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0006.jpg "Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041]](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0002.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0001.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Isotype control. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP1-97041-img0008.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane 1: Non-reduced Rabbit IgG Isotype control. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Isotype control. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0007.jpg)
![Flow Cytometry: Rat IgG Isotype Control [NBP1-71666] - Image from verified customer review.](http://images.novusbio.com/fullsize/Rat-IgG-Isotype-Control-Flow-Cytometry-NBP1-71666-img0001.jpg "Flow Cytometry: Rat IgG Isotype Control [NBP1-71666] - Image from verified customer review.")
![ELISA: Rat IgG Isotype Control [NBP1-71666] - ELISA plate was coated with serially diluted Rat IgG-UNLB. Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT and Mouse Anti-Rat IgM-BIOT followed by Streptavidin-HRP and quantified.](http://images.novusbio.com/fullsize/Rat-IgG-Isotype-Control-ELISA-NBP1-71666-img0002.jpg)
![ELISA: Human IgG ELISA Kit (Colorimetric) [NBP3-14790] - Samples were spiked with high concentrations of Human IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-14790_human-igg-elisa-kit-colorimetric-1322024168538.jpg "ELISA: Human IgG ELISA Kit (Colorimetric) [NBP3-14790] - Samples were spiked with high concentrations of Human IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human IgG ELISA Kit (Colorimetric) [NBP3-14790] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-14790_human-igg-elisa-kit-colorimetric-432024941518.jpg)
![ELISA: Rat IgG ELISA Kit (Chemiluminescence) [NBP3-00474] - Samples were spiked with high concentrations of Rat IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00474_rat-igg-elisa-kit-chemiluminescence-1322024162047.jpg "ELISA: Rat IgG ELISA Kit (Chemiluminescence) [NBP3-00474] - Samples were spiked with high concentrations of Rat IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IgG ELISA Kit (Chemiluminescence) [NBP3-00474] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00474_rat-igg-elisa-kit-chemiluminescence-432024924075.jpg)
MAB9794) coated on a Clear Polystyrene Microplate (Catalog # 
MAB9895) coated on a Clear Polystyrene Microplate (Catalog # 
MAB11013) coated on a Clear Polystyrene Microplate (Catalog # 
