| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3775-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96870-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97450-0.2ml | |||
| BSA Free | NBP1-97450-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12877-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61641-0.025mg | |||
![Western Blot: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Western-Blot-NB110-96870-img0003.jpg "Western Blot: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (NB110-96870) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Immunocytochemistry-Immunofluorescence-NB110-96870-img0007.jpg)
![Immunohistochemistry: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-96870) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Immunohistochemistry-NB110-96870-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (NB110-96870) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-Hyb-K41220A-Immunocytochemistry-Immunofluorescence-NB110-96870-img0008.jpg)
![Western Blot: HSP90 Antibody (2D12) [NBP1-97450] - Analysis of HSP90 mAb (2D12). Lane 1: MW marker, Lane 2: HSP90 (human) (native), Lane 3: HeLa HS, (cell lysate) Lane4: Vero Heat Shock Cell Lysate.](http://images.novusbio.com/fullsize/HSP90-Antibody-2D12-Western-Blot-NBP1-97450-img0001.jpg "Western Blot: HSP90 Antibody (2D12) [NBP1-97450] - Analysis of HSP90 mAb (2D12). Lane 1: MW marker, Lane 2: HSP90 (human) (native), Lane 3: HeLa HS, (cell lysate) Lane4: Vero Heat Shock Cell Lysate.")
![Western Blot: HSP90 Antibody (4B5) [NBP3-15145] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissueAll lanes: HSP90 antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 85 KDa.](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Western-Blot-NBP3-15145-img0005.jpg "Western Blot: HSP90 Antibody (4B5) [NBP3-15145] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissueAll lanes: HSP90 antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 85 KDa.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4B5) [NBP3-15145] - Immunofluorescence staining of NIH/3T3 cells with at HSP90 antibody 1:26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunocytochemistry-Immunofluorescence-NBP3-15145-img0001.jpg)
![Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145] - IHC image of HSP90 antibody diluted at 1:80 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunohistochemistry-NBP3-15145-img0004.jpg)
![Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145] - IHC image of HSP90 antibody diluted at 1:80 and staining in paraffin-embedded human endometrial cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunohistochemistry-NBP3-15145-img0003.jpg)
![Immunoprecipitation: HSP90 Antibody (4B5) [NBP3-15145] - Immunoprecipitating HSP90 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of HSP90 antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: HSP90 antibody (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/HSP90-Antibody-4B5-Immunoprecipitation-NBP3-15145-img0002.jpg)
![Western Blot: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - The Recombinant Human HSP90 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Western-Blot-NBP1-04301-img0004.jpg "Western Blot: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - The Recombinant Human HSP90 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - ICC/IF analysis of HSP90 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HSP90 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-04301-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Immunohistochemistry-Paraffin-NBP1-04301-img0002.jpg)
![Flow Cytometry: HSP90 Antibody (4F10) - BSA Free [NBP1-04301] - Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with NBP1-04301 at 2-5ug for 1x10^6 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/HSP90-Antibody-4F10-BSA-Free-Flow-Cytometry-NBP1-04301-img0008.jpg)
![Western Blot: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (NBP2-12877). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NBP2-12877) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-2G5-G3-Western-Blot-NBP2-12877-img0009.jpg "Western Blot: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (NBP2-12877). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NBP2-12877) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-2G5-G3-Immunocytochemistry-Immunofluorescence-NBP2-12877-img0007.jpg)
![Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Western-Blot-NBP3-16528-img0001.jpg "Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug HSP90 alpha antibody (NBP3-16528). Western blot was performed from the immunoprecipitate using HSP90 alpha antibody (NBP3-16528) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Immunoprecipitation-NBP3-16528-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Rat brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202511411441.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Mouse brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202511402778.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Human colon cancer tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-5220251255590.jpg)
![Western Blot: HSP90 Antibody (S08-8A1) [NBP3-15043] - Western blot detection of Hsp90 in Rat Brain, C6, 3T3, Hela cell lysates using Hsp90 Rabbit mAb (1:1000 diluted). Predicted band size: 85, 83kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/HSP90-Antibody-S08-8A1-Western-Blot-NBP3-15043-img0001.jpg "Western Blot: HSP90 Antibody (S08-8A1) [NBP3-15043] - Western blot detection of Hsp90 in Rat Brain, C6, 3T3, Hela cell lysates using Hsp90 Rabbit mAb (1:1000 diluted). Predicted band size: 85, 83kDa. Observed band size: 90kDa.")
![Immunohistochemistry-Paraffin: HSP90 Antibody (S08-8A1) [NBP3-15043] - Immunohistochemistry of HSP90 in paraffin-embedded Human tonsil using HSP90 Rabbit mAb at dilution 1/5](http://images.novusbio.com/fullsize/HSP90-Antibody-S08-8A1-Immunohistochemistry-Paraffin-NBP3-15043-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (S08-8A1) [NBP3-15043] - Immunofluorescence of Hsp90 (green) in Hela cells using NBP3-15043 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-15043_rabbit-hsp90-mab-s08-8a1-11320251514547.jpg)
![Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.](http://images.novusbio.com/fullsize/HSP90-alpha-[ac-Lys294]-Antibody-Western-Blot-NBP1-77944-img0005.jpg "Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.")
![Immunohistochemistry: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HSP90-alpha-[ac-Lys294]-Antibody-Immunohistochemistry-NBP1-77944-img0006.jpg)
![Western Blot: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Western-Blot-NB110-61641-img0007.jpg "Western Blot: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 (NB110-61641). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-61641) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Immunohistochemistry-NB110-61641-img0009.jpg)
![Immunohistochemistry: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 (NB110-61641). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-61641) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Immunohistochemistry-NB110-61641-img0010.jpg)
![Western Blot: HSP90 Antibody (4D1) [NBP3-15147] - Positive WB detected in: Jurkat whole cell lysate. All lanes: HSP90 antibody at 1.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 90 KDa.](http://images.novusbio.com/fullsize/HSP90-Antibody-4D1-Western-Blot-NBP3-15147-img0003.jpg "Western Blot: HSP90 Antibody (4D1) [NBP3-15147] - Positive WB detected in: Jurkat whole cell lysate. All lanes: HSP90 antibody at 1.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 90 KDa.")
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4D1) [NBP3-15147] - Immunofluorescence staining of NIH/3T3 cells with at HSP90 antibody 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/HSP90-Antibody-4D1-Immunocytochemistry-Immunofluorescence-NBP3-15147-img0001.jpg)
![Immunohistochemistry: HSP90 Antibody (4D1) [NBP3-15147] - IHC image of HSP90 antibody diluted at 1:190 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4D1-Immunohistochemistry-NBP3-15147-img0002.jpg)