HSP27: Novus Biologicals

NBP2-32972

HSP27 Antibody (G3.1)

(1 Review)

(5 Publications)

0.1 mg

NBP2-32972

Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffin-embedded human Breast Carcinome stained with HSP27 Monoclonal Antibody (G3.1)

Flow Cytometry: HSP27 Antibody (G3.1) [NBP2-32972] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (G3.1)followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: HSP27 Antibody (G3.1) [NBP2-32972] - Formalin-fixed. paraffim-embedded human prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)

Hsp90 inhibition reduces cell viability in a concentration-dependent manner and increases cytosolic Hsp70 and Hsp27 levels. (A, B) Toxicity assay of B16F10 (A) and LS174T (B) WT and LDH−/− cells treated with NVP-AUY922 (0, 5, 10, 50, 100 nM) for 24 h. (C, D) Representative immunoblot showing intracellular Hsp70, Hsp27 and beta -Actin levels in B16F10 (C) and LS174T (D) cells upon treatment with NVP-AUY922 (100 nM) for 24 h. Quantification of the heat shock protein (HSP) levels are shown in the adjacent bar chart (**p ≤ 0.01, ***p ≤ 0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35463341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ch, Pm, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Pm, Pm, Sh

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34621AF350
Alexa Fluor 405	NBP2-34621AF405
Alexa Fluor 488	NBP2-34621AF488
Alexa Fluor 532	NBP2-34621AF532
Alexa Fluor 594	NBP2-34621AF594
Alexa Fluor 647	NBP2-34621AF647
Alexa Fluor 700	NBP2-34621AF700
Alexa Fluor 750	NBP2-34621AF750
Alexa Fluor Plus 405	NBP2-34621AFP405
Alexa Fluor Plus 488	NBP2-34621AFP488
Alexa Fluor Plus 555	NBP2-34621AFP555
Alexa Fluor Plus 594	NBP2-34621AFP594
Alexa Fluor Plus 647	NBP2-34621AFP647
Alexa Fluor Plus 680	NBP2-34621AFP680
Allophycocyanin	NBP2-34621APC
Biotin	NBP2-34621B
CoraFluor 1	NBP2-34621CL1
DyLight 350	NBP2-34621UV
DyLight 405	NBP2-34621V
DyLight 488	NBP2-34621G
DyLight 550	NBP2-34621R
DyLight 594	NBP2-34621DL594
DyLight 650	NBP2-34621C
DyLight 680	NBP2-34621FR
DyLight 755	NBP2-34621IR
FITC	NBP2-34621F
HRP	NBP2-34621H
Janelia Fluor 525	NBP2-34621JF525
Janelia Fluor 549	NBP2-34621JF549
Janelia Fluor 646	NBP2-34621JF646
Janelia Fluor 669	NBP2-34621JF669
PE	NBP2-34621PE
PerCP	NBP2-34621PCP
mFluor Violet 450 SE	NBP2-34621MFV450
mFluor Violet 500 SE	NBP2-34621MFV500
mFluor Violet 610 SE	NBP2-34621MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-44973
Azide and BSA Free	NBP2-34621-0.1mg

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NBP1-75477

HSP27 Antibody - BSA Free

(1 Review)

(2 Publications)

0.1 ml

NBP1-75477

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.

Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ha

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-75477AF350
Alexa Fluor 405	NBP1-75477AF405
Alexa Fluor 488	NBP1-75477AF488
Alexa Fluor 532	NBP1-75477AF532
Alexa Fluor 594	NBP1-75477AF594
Alexa Fluor 647	NBP1-75477AF647
Alexa Fluor 700	NBP1-75477AF700
Alexa Fluor 750	NBP1-75477AF750
Alexa Fluor Plus 405	NBP1-75477AFP405
Alexa Fluor Plus 488	NBP1-75477AFP488
Alexa Fluor Plus 555	NBP1-75477AFP555
Alexa Fluor Plus 594	NBP1-75477AFP594
Alexa Fluor Plus 647	NBP1-75477AFP647
Alexa Fluor Plus 680	NBP1-75477AFP680
Allophycocyanin	NBP1-75477APC
Biotin	NBP1-75477B
CoraFluor 1	NBP1-75477CL1
DyLight 350	NBP1-75477UV
DyLight 405	NBP1-75477V
DyLight 488	NBP1-75477G
DyLight 550	NBP1-75477R
DyLight 594	NBP1-75477DL594
DyLight 650	NBP1-75477C
DyLight 680	NBP1-75477FR
DyLight 755	NBP1-75477IR
FITC	NBP1-75477F
HRP	NBP1-75477H
Janelia Fluor 525	NBP1-75477JF525
Janelia Fluor 549	NBP1-75477JF549
Janelia Fluor 646	NBP1-75477JF646
Janelia Fluor 669	NBP1-75477JF669
PE	NBP1-75477PE
PerCP	NBP1-75477PCP
mFluor Violet 450 SE	NBP1-75477MFV450
mFluor Violet 500 SE	NBP1-75477MFV500
mFluor Violet 610 SE	NBP1-75477MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-75477SS

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NB120-5579

HSP27 Antibody

(1 Review)

(2 Publications)

100μg

NB120-5579

Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.

Reactivity:

Hu, Rt, Pm, Ca

Species Glossary

Reactivity: Hu, Rt, Pm, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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AF2314

HSP27 [p Ser78, p Ser82, p Ser82] Antibody [Unconjugated]

(4 Publications)

50 μg

AF2314

HAF008). A specific band was detected for Phospho-HSP27 (S78/S82) at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line untreated (-) or treated (+) with 100 J/m<SUP>2</SUP>UV-C for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2314), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for Phospho-HSP27 (S78/S82) at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line untreated (-) or treated (+) with 100 J/m²UV-C for 30 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2314), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-HSP27 (S78/S82) at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>HSP27 phosphorylated at S78/S82 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line unstimulated (lower panel) or stimulated with 20 mJ/cm<sup>2</sup>ultraviolet radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-HSP27 (S78/S82) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2314) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Enrichment of TICs decreases PP2A activity and increases Hsp27 activation in other solid tumors.CCS and HCW colorectal cancer cells, A549 lung cancer cells, HTB186 medulloblastoma cells and SAS, oral cancer cells were continually cultured under serum depletion in the presence of EGF (10 ng/mL) and FGF2 (10 ng/mL) to enrich TICs (E+F) or in serum-containing medium (CTR) as a control. (A) Immunoblots for pluripotent markers. (B) Immunoblots for phosphorylated PP2A (pPP2A), PP2A and Hsp27. (C) Immunoblots for caspase 9 and 3. (D) Schema demonstrates the antiapoptosis pathway of colorectal TICs in response to in vitro hypoxia and serum depletion. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0049605), licensed under a CC-BY license. Not internally tested by R&D Systems.

Decrease in PP2A activity increases phosphorylation of p38MAPK, pMAPKAPK2 and Hsp27 in CD133+ cells.(A upper panel) Immunoblot analysis and (A lower panel) PP2A activity analysis for HT-29 CD133+ and CD133− cells after MACS separation at day 4 of exposure to hypoxia and serum depletion. (B upper panel) Immunoblot analysis and (B lower panel) PP2A activity of CCS cells cultured under serum depletion in the presence of EGF (10 ng/mL) and FGF2 (10 ng/mL) (EGF+FGF2) for indicated time periods. The fold values are relative to the band intensity of the 5 day treatment. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23185379), licensed under a CC-BY license. Not internally tested by R&D Systems.

The involvement of Hsp27 activation in the anti-apoptosis pathway of CD133+ cells.Cells were lentivirally transfected with Hsp27 shRNA (shR1 and shR2) or scrambled shRNA, then exposed to hypoxia and serum depletion. CD133+ and CD133− cells were isolated using MACS separation 4 days later. (A) Immunoblot analysis for HT-29 protein levels of CD133+ and CD133− cells after MACS separation. (B) The percentage of CD133+ cells were analyzed by flow cytometry and the increased CD133+ percentage compared to CD133+ percentage in normoxia and growth medium was calculated. (C) Cells were re-exposed to hypoxia and serum depletion for 1 day, followed by TUNEL staining. (D-F) HT-29 cells were exposed to serum depletion and hypoxia in the presence of Quercetin, KRIBB3, or DMSO (vehicle control) for 4 days. (D) CD133+ cell were isolated and the protein levels were analyzed by western blotting. (E) The increased percentage of CD133+ cells and (F) apoptosis of CD133+ cells were analyzed by flow cytometry and TUNEL staining, respectively. Error bars represent standard deviations. (**p<0.01 compared with the scrambled as determined by the Student’s t test.) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23185379), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2314-SP

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AF1580

HSP27 Antibody [Unconjugated]

(6 Publications)

50 μg

AF1580

HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/ml of Rabbit Anti-Human HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/ml of Rabbit Anti-Human HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (<a class=

HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1580-SP

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AF15801

HSP27 Antibody [Unconjugated]

(3 Publications)

50 μg

AF15801

HAF109). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 33 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF15801-SP

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NB110-96431

HSP27 Antibody (8A7) - BSA Free

0.1 mg

NB110-96431

Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.

Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Gp, Ha, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Gp, Ha, Sh

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-96431AF350
Alexa Fluor 405	NB110-96431AF405
Alexa Fluor 488	NB110-96431AF488
Alexa Fluor 532	NB110-96431AF532
Alexa Fluor 594	NB110-96431AF594
Alexa Fluor 647	NB110-96431AF647
Alexa Fluor 700	NB110-96431AF700
Alexa Fluor 750	NB110-96431AF750
Alexa Fluor Plus 405	NB110-96431AFP405
Alexa Fluor Plus 488	NB110-96431AFP488
Alexa Fluor Plus 555	NB110-96431AFP555
Alexa Fluor Plus 594	NB110-96431AFP594
Alexa Fluor Plus 647	NB110-96431AFP647
Alexa Fluor Plus 680	NB110-96431AFP680
Allophycocyanin	NB110-96431APC
Biotin	NB110-96431B
CoraFluor 1	NB110-96431CL1
DyLight 350	NB110-96431UV
DyLight 405	NB110-96431V
DyLight 488	NB110-96431G
DyLight 550	NB110-96431R
DyLight 594	NB110-96431DL594
DyLight 650	NB110-96431C
DyLight 680	NB110-96431FR
DyLight 755	NB110-96431IR
FITC	NB110-96431F
HRP	NB110-96431H
Janelia Fluor 525	NB110-96431JF525
Janelia Fluor 549	NB110-96431JF549
Janelia Fluor 646	NB110-96431JF646
Janelia Fluor 669	NB110-96431JF669
PE	NB110-96431PE
PerCP	NB110-96431PCP
mFluor Violet 450 SE	NB110-96431MFV450
mFluor Violet 500 SE	NB110-96431MFV500
mFluor Violet 610 SE	NB110-96431MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-96431-0.025mg

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NBP2-32865

HSP27 Antibody (SPM252)

0.1 mg

NBP2-32865

Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 Mouse Monoclonal Antibody (SPM252).

Flow Cytometry: HSP27 Antibody (SPM252) [NBP2-32865] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (SPM252).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (20X) stained with HSP27 MAb (SPM252)

Immunohistochemistry-Paraffin: HSP27 Antibody (SPM252) [NBP2-32865] - Formalin-paraffin breast carcinoma (10X) stained with HSP27 MAb (SPM252))

Reactivity:

Hu, Mu, Rt, Ch, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Pm, Sh

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34751AF350
Alexa Fluor 405	NBP2-34751AF405
Alexa Fluor 488	NBP2-34751AF488
Alexa Fluor 532	NBP2-34751AF532
Alexa Fluor 594	NBP2-34751AF594
Alexa Fluor 647	NBP2-34751AF647
Alexa Fluor 700	NBP2-34751AF700
Alexa Fluor 750	NBP2-34751AF750
Alexa Fluor Plus 405	NBP2-34751AFP405
Alexa Fluor Plus 488	NBP2-34751AFP488
Alexa Fluor Plus 555	NBP2-34751AFP555
Alexa Fluor Plus 594	NBP2-34751AFP594
Alexa Fluor Plus 647	NBP2-34751AFP647
Alexa Fluor Plus 680	NBP2-34751AFP680
Allophycocyanin	NBP2-34751APC
Biotin	NBP2-34751B
CoraFluor 1	NBP2-34751CL1
DyLight 350	NBP2-34751UV
DyLight 405	NBP2-34751V
DyLight 488	NBP2-34751G
DyLight 550	NBP2-34751R
DyLight 594	NBP2-34751DL594
DyLight 650	NBP2-34751C
DyLight 680	NBP2-34751FR
DyLight 755	NBP2-34751IR
FITC	NBP2-34751F
HRP	NBP2-34751H
Janelia Fluor 525	NBP2-34751JF525
Janelia Fluor 549	NBP2-34751JF549
Janelia Fluor 646	NBP2-34751JF646
Janelia Fluor 669	NBP2-34751JF669
PE	NBP2-34751PE
PerCP	NBP2-34751PCP
mFluor Violet 450 SE	NBP2-34751MFV450
mFluor Violet 500 SE	NBP2-34751MFV500
mFluor Violet 610 SE	NBP2-34751MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-44974
Azide and BSA Free	NBP2-34751-0.1mg

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NBP2-25149

HSP27 Antibody (6H11) - BSA Free

(2 Publications)

0.1 ml

NBP2-25149

Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria

Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.

Reactivity:

Hu, Mu(-), Rt(-)

Species Glossary

Reactivity: Hu, Mu(-), Rt(-)

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25149AF350
Alexa Fluor 405	NBP2-25149AF405
Alexa Fluor 488	NBP2-25149AF488
Alexa Fluor 532	NBP2-25149AF532
Alexa Fluor 594	NBP2-25149AF594
Alexa Fluor 647	NBP2-25149AF647
Alexa Fluor 700	NBP2-25149AF700
Alexa Fluor 750	NBP2-25149AF750
Alexa Fluor Plus 405	NBP2-25149AFP405
Alexa Fluor Plus 488	NBP2-25149AFP488
Alexa Fluor Plus 555	NBP2-25149AFP555
Alexa Fluor Plus 594	NBP2-25149AFP594
Alexa Fluor Plus 647	NBP2-25149AFP647
Alexa Fluor Plus 680	NBP2-25149AFP680
Allophycocyanin	NBP2-25149APC
Biotin	NBP2-25149B
CoraFluor 1	NBP2-25149CL1
DyLight 350	NBP2-25149UV
DyLight 405	NBP2-25149V
DyLight 488	NBP2-25149G
DyLight 550	NBP2-25149R
DyLight 594	NBP2-25149DL594
DyLight 650	NBP2-25149C
DyLight 680	NBP2-25149FR
DyLight 755	NBP2-25149IR
FITC	NBP2-25149F
HRP	NBP2-25149H
Janelia Fluor 525	NBP2-25149JF525
Janelia Fluor 549	NBP2-25149JF549
Janelia Fluor 646	NBP2-25149JF646
Janelia Fluor 669	NBP2-25149JF669
PE	NBP2-25149PE
PerCP	NBP2-25149PCP
mFluor Violet 450 SE	NBP2-25149MFV450
mFluor Violet 500 SE	NBP2-25149MFV500
mFluor Violet 610 SE	NBP2-25149MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25149SS

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NBP2-44975

HSP27 Antibody (HSPB1/774)

0.1 mg

NBP2-44975

Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

Flow Cytometry: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/774) [NBP2-44975] - Human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

Reactivity:

Hu, Mu, Rt, Ch, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Pm, Sh

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-48002AF350
Alexa Fluor 405	NBP2-48002AF405
Alexa Fluor 488	NBP2-48002AF488
Alexa Fluor 532	NBP2-48002AF532
Alexa Fluor 594	NBP2-48002AF594
Alexa Fluor 647	NBP2-48002AF647
Alexa Fluor 700	NBP2-48002AF700
Alexa Fluor 750	NBP2-48002AF750
Alexa Fluor Plus 405	NBP2-48002AFP405
Alexa Fluor Plus 488	NBP2-48002AFP488
Alexa Fluor Plus 555	NBP2-48002AFP555
Alexa Fluor Plus 594	NBP2-48002AFP594
Alexa Fluor Plus 647	NBP2-48002AFP647
Alexa Fluor Plus 680	NBP2-48002AFP680
Allophycocyanin	NBP2-48002APC
Biotin	NBP2-48002B
CoraFluor 1	NBP2-48002CL1
DyLight 350	NBP2-48002UV
DyLight 405	NBP2-48002V
DyLight 488	NBP2-48002G
DyLight 550	NBP2-48002R
DyLight 594	NBP2-48002DL594
DyLight 650	NBP2-48002C
DyLight 680	NBP2-48002FR
DyLight 755	NBP2-48002IR
FITC	NBP2-48002F
HRP	NBP2-48002H
Janelia Fluor 525	NBP2-48002JF525
Janelia Fluor 549	NBP2-48002JF549
Janelia Fluor 646	NBP2-48002JF646
Janelia Fluor 669	NBP2-48002JF669
PE	NBP2-48002PE
PerCP	NBP2-48002PCP
mFluor Violet 450 SE	NBP2-48002MFV450
mFluor Violet 500 SE	NBP2-48002MFV500
mFluor Violet 610 SE	NBP2-48002MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-48002-0.1mg
IHC-Prediluted	NBP2-48186

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NBP2-24533

HSP27 Antibody

0.1 mg

NBP2-24533

Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity:

Hu, Ca, Eq, Pm, Mu, Rt

Species Glossary

Reactivity: Hu, Ca, Eq, Pm, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6426A

Alt Catalog# IMG-6426A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBC1-18364

Recombinant Human HSP27 Protein

(1 Publication)

0.1 mg

NBC1-18364

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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