| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26572-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08920 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08921 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54575-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54415-100ug | |||
![Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Immunoprecipitating HNF-3 alpha/FoxA1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26572 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26572 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26572_rabbit-hnf-3-alpha-foxa1-mab-3b2-2622024132377.jpg "Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Immunoprecipitating HNF-3 alpha/FoxA1 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26572 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26572 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26572_rabbit-hnf-3-alpha-foxa1-mab-3b2-262202414471534.jpg)
![Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Image of HNF-3 alpha/FoxA1 Antibody (3B2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26572_rabbit-hnf-3-alpha-foxa1-mab-3b2-2622024152243.jpg)
![Western Blot: HNF-3 alpha/FoxA1 Antibody (3B2) [NBP3-26572] - Positive Western Blot detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate. All lanes: HNF-3 alpha/FoxA1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50, 46 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26572_rabbit-hnf-3-alpha-foxa1-mab-3b2-282202411472132.jpg)
![Western Blot: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Western blot analysis of HNF-3 alpha/FoxA1 on LNCaP cell lysates with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32346_mouse-hnf-3-alpha-foxa1-mab-a2e8-r-278202415212490.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Western blot analysis of HNF-3 alpha/FoxA1 on LNCaP cell lysates with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32346) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32346) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32346_mouse-hnf-3-alpha-foxa1-mab-a2e8-r-298202414333671.jpg)
![Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (A2E8-R) [NBP3-32346] - Immunocytochemistry analysis of LNCaP cells labeling HNF-3 alpha/FoxA1 with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HNF-3 alpha/FoxA1 antibody (NBP3-32346) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32346_mouse-hnf-3-alpha-foxa1-mab-a2e8-r-2920241192352.jpg)
![Western Blot: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Western blot detection of HNF-3 alpha/FoxA1 in C6 cell lysates using NBP3-19637 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-S04-5A7-Western-Blot-NBP3-19637-img0002.jpg "Western Blot: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Western blot detection of HNF-3 alpha/FoxA1 in C6 cell lysates using NBP3-19637 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 49kDa.")
![Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (S04-5A7) [NBP3-19637] - Immunofluorescence of HNF-3 alpha/FoxA1 (green) in MCF-7 using NBP3-19637 at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19637_rabbit-hnf-3-alpha-foxa1-mab-s04-5a7-113202515145456.jpg)
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HNF-3 alpha/FoxA1 Mouse Recombinant Monoclonal Antibody (rHNF-3 alpha/FoxA1/1515).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-rFOXA1-1515-Immunohistochemistry-Paraffin-NBP3-07177-img0002.jpg "Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HNF-3 alpha/FoxA1 Mouse Recombinant Monoclonal Antibody (rHNF-3 alpha/FoxA1/1515).")
![Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (rFOXA1/1515) [NBP3-07177] - Flow Cytometric Analysis of PFA-fixed permeabilized MCF-7 cells using HNF-3 alpha/FoxA1 Mouse Recombinant Monoclonal Antibody (rHNF-3 alpha/FoxA1/1515). followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-rFOXA1-1515-Flow-Cytometry-NBP3-07177-img0001.jpg)
![Immunoprecipitation:HNF-3 alpha/FoxA1 AntibodyNBP3-29853] -Detection of human FOXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXA1 antibody NBP3-29853 (lot NBP3-29853-1) used for IP at 6 µg per reaction. FOXA1 was also immunoprecipitated by rabbit anti-FOXA1 antibody BL20490. For blotting immunoprecipitated FOXA1, NBP3-29853 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29853_rabbit-hnf-3-alpha-foxa1-pab-301220241727488.jpg "Immunoprecipitation:HNF-3 alpha/FoxA1 AntibodyNBP3-29853] -Detection of human FOXA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXA1 antibody NBP3-29853 (lot NBP3-29853-1) used for IP at 6 µg per reaction. FOXA1 was also immunoprecipitated by rabbit anti-FOXA1 antibody BL20490. For blotting immunoprecipitated FOXA1, NBP3-29853 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Rabbit HNF-3 alpha/FoxA1 pAb [Unconjugated] [NBP3-43591] - Analysis of A549 cells using Rabbit HNF-3 alpha/FoxA1 pAb.](http://images.novusbio.com/fullsize/antibody/nbp3-43591_rabbit-hnf-3-alpha-foxa1-pab-unconjugated-western-blot-2312025113543..jpg "Western Blot: Rabbit HNF-3 alpha/FoxA1 pAb [Unconjugated] [NBP3-43591] - Analysis of A549 cells using Rabbit HNF-3 alpha/FoxA1 pAb.")
![SDS-Page: Recombinant Human HNF-3 alpha/FoxA1 Protein [H00003169-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003169-P01-1.jpg "SDS-Page: Recombinant Human HNF-3 alpha/FoxA1 Protein [H00003169-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: FOXA1 Overexpression Lysate (Adult Normal) [NBL1-10798] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXA1.](http://images.novusbio.com/fullsize/FOXA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10798-img0002.jpg "Western Blot: FOXA1 Overexpression Lysate (Adult Normal) [NBL1-10798] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXA1.")
![HNF-3 alpha/FoxA1 Antibody (FOXA1/2230R) [Alexa Fluor® 350] [NBP3-08921AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.](http://images.novusbio.com/fullsize/nbp3-08921af350_rabbit-monoclonal-hnf-3-alpha-foxa1-antibody-foxa1-2230r-alexa-fluor-350-288202315212763.jpg "HNF-3 alpha/FoxA1 Antibody (FOXA1/2230R) [Alexa Fluor® 350] [NBP3-08921AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.")
![HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [Alexa Fluor® 350] [NBP2-54575AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.](http://images.novusbio.com/fullsize/nbp2-54575af350_mouse-monoclonal-hnf-3-alpha-foxa1-antibody-foxa1-1514-alexa-fluor-350-288202315341943.jpg "HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [Alexa Fluor® 350] [NBP2-54575AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.")