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![Immunocytochemistry/Immunofluorescence: GPR81 Antibody [NLS2095] - LPS increases glycolysis as well as lactate production by BM neutrophils. MCT4, MCT1, and GPR81 (yellow) distribution on BM CD11b+ (green)/Ly6G+ (red) neutrophils visualized and quantified by ImageStream analysis. Images are from one representative experiment out of three. Scale bar indicates 7 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-17402-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunocytochemistry-Immunofluorescence-NLS2095-img0010.jpg "Immunocytochemistry/Immunofluorescence: GPR81 Antibody [NLS2095] - LPS increases glycolysis as well as lactate production by BM neutrophils. MCT4, MCT1, and GPR81 (yellow) distribution on BM CD11b+ (green)/Ly6G+ (red) neutrophils visualized and quantified by ImageStream analysis. Images are from one representative experiment out of three. Scale bar indicates 7 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-17402-2) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Brain, Substantia Nigra, pigmented and nonpigmented neurons.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0009.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human anterior pituitary.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0004.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0005.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human pancreas, carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0007.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Breast, Carcinoma](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0008.jpg)
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Stable knockdown of GPR81 in MDA-MB-231 cells. Using a lentiviral system, GPR81 was stably knocked down in MDA-MB-231 cells with GPR81-specific short hairpin RNA (shGPR81 #1) & compared with cells containing scrambled shRNA as a control (shNT). Knockdown of GPR81 was confirmed by (a) western blotting & (b) RT-qPCR. The GPR81, MCT1, & MCT4 mRNA levels in the shGPR81 #1 cells are presented as the fold increase compared with those in shNT cells. Data are presented as the means ± s.d. (n = 3/group). **p < 0.01; Student’s t-test. (c) Lactate production in shNT & shGPR81 #1 cells. The cells were cultured for 24 h & lactate concentrations in the supernatants were determined (n = 3/group). **p < 0.01 (vs. shNT cells); Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-310202415334917.jpg)
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-31020241613628.jpg)
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-310202416114765.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0005.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Analysis of anti-FKSG80 / GPR81 antibody with pituitary, anterior at 3 ug/ ml.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0001.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Analysis of anti-FKSG80 / GPR81 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human pancreas islet of Langerhans. Imunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0003.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2091] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2091-img0004.jpg)
![Western Blot: GPR81 Antibody [NBP1-51956] - Analysis of GPR81 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-51956 at 0.5g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GPR81-Antibody-Western-Blot-NBP1-51956-img0002.jpg "Western Blot: GPR81 Antibody [NBP1-51956] - Analysis of GPR81 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-51956 at 0.5g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: GPR81 Antibody [NBP3-15999] - Analysis of Mouse brain, using HCAR1 antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15999_rabbit-gpr81-pab-western-blot-182023103514..jpg "Western Blot: GPR81 Antibody [NBP3-15999] - Analysis of Mouse brain, using HCAR1 antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NBP1-02369] - Staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/GPR81-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02369-img0001.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NBP1-02369] - Staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.")