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![Western Blot: CHD5 Antibody [NBP3-12985] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD5 antibody (NBP3-12985) diluted at 1:1000.](http://images.novusbio.com/fullsize/CHD5-Antibody-Western-Blot-NBP3-12985-img0002.jpg "Western Blot: CHD5 Antibody [NBP3-12985] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD5 antibody (NBP3-12985) diluted at 1:1000.")
![Western Blot: CHD5 Antibody [NBP3-12985] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD5 antibody (NBP3-12985) diluted at 1:1000.](http://images.novusbio.com/fullsize/CHD5-Antibody-Western-Blot-NBP3-12985-img0001.jpg)
![Western Blot: CHD5 Antibody [NBP3-18636] - Figure 2. Western blot analysis using the antibody directed against CHD5 HeLa cells were transfected with CHD5 and analysed by Western blot. Western blot was performed using 15 to 30 ug nuclear extracts and the antibody against human CHD5 . The antibody was diluted 1:1,000 in TBS-Tween containing 2% milk powder. The position of the protein of interest (expected size: 223 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells. Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.](http://images.novusbio.com/fullsize/CHD5-Antibody-Western-Blot-NBP3-18636-img0001.jpg "Western Blot: CHD5 Antibody [NBP3-18636] - Figure 2. Western blot analysis using the antibody directed against CHD5 HeLa cells were transfected with CHD5 and analysed by Western blot. Western blot was performed using 15 to 30 ug nuclear extracts and the antibody against human CHD5 . The antibody was diluted 1:1,000 in TBS-Tween containing 2% milk powder. The position of the protein of interest (expected size: 223 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells. Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.")
![ELISA: CHD5 Antibody [NBP3-18636] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human CHD5 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:93,500.](http://images.novusbio.com/fullsize/CHD5 Antibody-ELISA-NBP3-18636-img0002.jpg)
![Immunohistochemistry: CHD5 Antibody [NBP2-49186] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/CHD5-Antibody-Immunohistochemistry-NBP2-49186-img0001.jpg "Immunohistochemistry: CHD5 Antibody [NBP2-49186] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.")
![ELISA: Human CHD5 ELISA Kit (Colorimetric) [NBP2-75269] - Samples were spiked with high concentrations of Human CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75269_human-chd5-elisa-kit-colorimetric-132202416251774.jpg "ELISA: Human CHD5 ELISA Kit (Colorimetric) [NBP2-75269] - Samples were spiked with high concentrations of Human CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CHD5 ELISA Kit (Colorimetric) [NBP2-75269] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75269_human-chd5-elisa-kit-colorimetric-4320249115658.jpg)
![ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Samples were spiked with high concentrations of Rat CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75271_rat-chd5-elisa-kit-colorimetric-13220241612138.jpg "ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Samples were spiked with high concentrations of Rat CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75271_rat-chd5-elisa-kit-colorimetric-432024955076.jpg)
![ELISA: Mouse CHD5 ELISA Kit (Colorimetric) [NBP2-75270] - Samples were spiked with high concentrations of Mouse CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75270_mouse-chd5-elisa-kit-colorimetric-132202416102292.jpg "ELISA: Mouse CHD5 ELISA Kit (Colorimetric) [NBP2-75270] - Samples were spiked with high concentrations of Mouse CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CHD5 ELISA Kit (Colorimetric) [NBP2-75270] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75270_mouse-chd5-elisa-kit-colorimetric-432024963950.jpg)
![Immunocytochemistry/Immunofluorescence: CHD5 Antibody [NBP3-06267] - Immunofluorescence staining of CHD5 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06267 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06267_rabbit-polyclonal-chd5-antibody-23520252145176.png "Immunocytochemistry/Immunofluorescence: CHD5 Antibody [NBP3-06267] - Immunofluorescence staining of CHD5 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06267 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.")