LRIG1: Novus Biologicals

AF3688

LRIG1 Antibody [Unconjugated]

100 μg

AF3688

HAF109). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

<b>Biological Strategies Validation. </b>bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

JunB expression during skin maturation and upon stress. a Representative microphotographs with double immunostaining for JunB (green) and FABP5 (red), indicative of sebaceous glands, in skin derived from 1, 3, and 5 days old mice. Inset showing magnified view of a sebaceous gland. Scale bars, 50 µm. b Immunostaining of JunB (green) and FABP5 (red) in 60 days old mice. Inset showing magnified view. c Representative microphotographs with double immunostaining of JunB (green) and FABP5 (red) in dorsal skin of 60 days old mice following hair plucking or d after topical TPA application, a potent inducer of proliferation. e Immunostaining of JunB (green) and LRIG1 or CD34 (red) in dorsal skin of 60 days old mice following hair plucking. f Immunostaining of JunB (green) and FABP5 (red) in adult human skin. E, epidermis; D, dermis; HF, hair follicle; SG, sebaceous gland; SD, sebaceous duct; HS, hair shaft. Asterisk indicates hair shaft autofluorescence. Dashed line indicates the epidermal-dermal junction. Scale bars, 50 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143626), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lrigs are co-expressed in the embryonic inner ear.(A) Diagram of the immature inner ear structure at E12.5 (left) and the mature structure at E16 (right), with schematic cross sections cut transverse to the ear at each age shown below. For the E16 cross section, the sensory epithelia are labeled red and the neurons green. In situs for Lrig1 (B) and X-gal staining for Lrig3-beta geo activity (C) show overlapping expression for Lrig1 and Lrig3 in the atrium (arrowhead) and the non-sensory domain of the cochlea. On the other hand, Lrig2-beta geo is active throughout the developing otic epithelium (D). c = cochlea, ed = endolymphatic duct, lp = lateral pouch, vp = vertical pouch. Scale bar = 50 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24086156), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lrig1 and Lrig2-beta geo are co-expressed in non-sensory tissues and in the vestibular ganglion.Transverse sections through Lrig2+/− tissue at E12.5 (A–C), E16.5 (D–G), and P15 (H–K) were double labeled with combinations of antibodies to Lrig1, beta -galactosidase (to detect Lrig2-beta geo), Sox2, and neurofilament (NF). (A) At E12.5, Lrig1 was detected in the atrium (bracket), while Lrig2-beta geo was present throughout the otic epithelium and surrounding mesenchyme (A″), overlapping with Lrig1 in the atrium (B). Within the atrium, Lrig1 was present in non-sensory tissues that flank Sox-2 positive sensory regions (arrowheads, C–C″). This expression was maintained at E16.5, with Lrig1 in the transitional epithelium adjacent to the utricular macula (arrowhead, D′) and in the extramacular epithelium of the saccule (arrowhead, F′), as well as in vestibular projections to the utricle and lateral crista (arrow, D′). Lrig2-beta geo, on the other hand, continued to be expressed broadly in both sensory and non-sensory portions of the vestibular organs at E16.5 (E′, G′). After the onset of hearing (P15), Lrig1 was expressed in NF-positive fibers innervating the utricular and saccular maculae (arrows, I, J), whereas Lrig2-beta geo was enriched in all vestibular sensory epithelia (I′,J′,K), which were recognized by the presence of NF labeled projections. c = crista, cd = cochlear duct, ed = endolymphatic duct, hb = hindbrain, lp = lateral pouch, sg = spiral ganglion, sm = saccular macula, um = utricular macule, vg = vestibular ganglion, vp = vertical pouch, VIIIV = vestibular division of the eighth cranial nerve. Scale bar = 40 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24086156), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lrig1 and Lrig2-beta geo are co-expressed in the non-sensory region of the cochlea.Transverse sections through Lrig2+/− tissue at E12.5 (A), E16.5 (B), and P15 (C) were double labeled with antibodies to Lrig1, beta -galactosidase, and NF. (A) At E12.5, staining was evident in the non-sensory region of the cochlear epithelium (asterisk) and the mesenchyme surrounding the spiral ganglion. (B) At E16.5, Lrig1 was detected in the medial wall of the cochlea, which will form the inner sulcus and Reissner's membrane (asterisk). (C) At P15, Lrig1 was found in the base of Reissner's membrane (asterisk), with localization to the cell surface (inset). In contrast, at E12.5 and 16.5, Lrig2-beta geo was found broadly in the cochlear epithelium and surrounding mesenchyme (A′–B′). At P15, expression was enriched in spiral ganglion neurons and in the organ of Corti (C′). cd = cochlear duct, m = mesenchyme, oC = organ of Corti, rm = Reissner's membrane, sg = spiral ganglion. Scale bar = 40 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24086156), licensed under a CC-BY license. Not internally tested by R&D Systems.

Association of Oct1 with normal somatic stem cells.A. IF images of cross-sections from grossly uninvolved colon margins of a male familial adenomatous polyposis patient. Frozen sections were stained with mouse anti-Oct1 antibodies (Millipore MAB5434) and co-stained with TO-PRO. Crypts are shown in cross-section. White dashed circle highlights a crypt. Arrows indicate cells staining strongly for Oct1. B. IF images of colon crypt sections from a normal male individual. Sections were stained with DAPI, and anti-Oct1 and anti-ALDH1a1 antibodies. Merged images are shown at right. White dashed lines highlight crypts. Examples of cells co-staining with Oct1 and ALDH1a1 are highlighted with yellow arrows. An example cell staining with ALDH1a1 only is highlighted with an asterisk. Inset at lower right-hand corner is a digital magnification of the central portion of the image. Sections were formalin-fixed and paraffin-embedded. C. Frozen mouse colon tissue sections were stained with DAPI, and anti-Lrig1 and anti-Oct1 antibodies. IF images of longitudinal sections are shown. White dashed lines highlight the crypt. D. IF images of mouse small intestine sections. Sections were stained with DAPI and anti-Oct1 antibodies. Merged images are shown at right. White dashed lines highlight a crypt. Inset at upper right-hand corner is a digital magnification of the central portion of the image. Sections were formalin-fixed and paraffin-embedded. E. IF images of cross-sectional duodenum sections from a normal C57BL/6 mouse. Frozen sections were stained with DAPI and anti-Oct1 and anti-Lgr5 antibodies. Merged images are shown at right. Examples of co-staining cells are highlighted with yellow arrows. White dashed lines highlights crypts. F. Longitudinal sections. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23144633), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lrig1 KO GSCs show impaired BMP signalling (A) Phase-contrast imaging of Lrig1 WT and Lrig1 KO GSCs treated with EGF/FGF or BMP4 for 3 days. (B) Immunoblot for LRIG1, EGFR, OLIG2, CD9, ID1 and GAPDH expression in Lrig1 WT and Lrig1 KO GSCs treated with EGF/FGF or BMP4 for 3 days. (C) Immunoblot and quantification for PCNA and GAPDH in Lrig1 WT and Lrig1 KO GSCs grown in EGF/FGF-2 or treated with 5 ng/ml or 10 ng/ml of BMP4 for 3 days. Quantification shown relative to GAPDH and Lrig1 WT EGF/FGF-2 control. (D) Immunoblot and quantification for MCM2 and GAPDH in Lrig1 WT and Lrig1 KO GSCs grown in EGF/FGF-2 or treated with 5 ng/ml or 10 ng/ml of BMP4 for 3 days. Quantification shown relative to GAPDH and Lrig1 WT EGF/FGF-2 control. (E) Immunoblot for LRIG1, pSMAD1/5, ID1, BLBP, pERK1/2 and GAPDH in Lrig1 WT and Lrig1 KO GSCs treated with different dosages of BMP4 for 3 days. Dosages in ng/ml. Protein band sizes shown in kDa. (F) Flow cytometry plots showing strategy to select Lrig1-positive cells in Lrig1 KO GSCs after reintroduction of mLrig1-FLAG. (G) Immunostaining for pSMAD1/5 (red) and nuclear counterstaining with DAPI (blue). +Lrig1-FLAG refer to the sorted populations. ICC was performed on Fucci PE Lrig1 WT, Fucci PE Lrig1 KO and the populations sorted for Lrig1-FLAG following expansion. (H) Quantification of the percentage of pSMAD 1/5 in the different conditions (n = 3) Unpaired two-tailed t-tests. Scale bar in (A) is 50 μm and (H) is 20 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36420140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Overexpression of Lrig1 reduces GSC proliferation. (A) Representative fluorescence imaging of DAPI stained colonies using Operetta high-content analysis system for NPE-IE control and NPE-IE mLrig1 overexpressing cells. (B) Quantification of the number of colonies formed in single cell colony forming assays by NPE-IE control and NPE-IE mLrig1 overexpressing cells (n = 3) (C) Quantification of the size of colonies formed in single cell colony forming assays by NPE-IE control and NPE-IE mLrig1 overexpressing cells (n = 3) p = 0.0456. Each dot represents a single colony. (D) Immunoblot and quantification of LRIG1, OLIG2, ID1, CD9 and GAPDH (loading control) in NPE-IE control and NPE-IE mLrig1 overexpressing GSCs treated with EGF/FGF or BMP4 for 3 days. Quantification shown relative to GAPDH and NPE-IE EGF/FGF-2 control. Scale bar in (A) is 500 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36420140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lrig1 regulates proliferation of GSCs. (A) Flow analysis of Fucci mCherry-Cdt1 and aVenus-hGem reporters in Lrig1 WT and Lrig1 KO mouse GSCs. (n = 3). (B) Quantification of the number of colonies formed in single cell colony forming assays by Fucci NSCs, Fucci PE Lrig1 WT (GSCs with p53 KO and EGFRvIII overexpression) and Fucci PE Lrig1 KO. n = 3 (mean ± SD). (C) Quantification of the size of colonies formed in single cell colony forming assays by Fucci NSC, Fucci PE Lrig1 WT (GSCs with p53 KO and EGFRvIII overexpression) and Fucci PE Lrig1 KO as assessed by DAPI (n = 3 independent experiments, median ± SD, each dot represents a single colony). p value = 0.0456. (D) Representative fluorescence imaging of DAPI stained colonies using Operetta high-content analysis system. Scale bar in (D) is 1,500 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36420140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3688U-100UG
Alexa Fluor 405	AF3688V-100UG
Alexa Fluor 488	FAB3688G
Alexa Fluor 532	AF3688X-100UG
Alexa Fluor 594	AF3688T-100UG
Alexa Fluor 647	AF3688R-100UG
Alexa Fluor 700	AF3688N-100UG
Alexa Fluor 750	AF3688S-100UG
Alexa Fluor Plus 405	AF3688AFP405-100UG
Alexa Fluor Plus 488	AF3688AFP488-100UG
Alexa Fluor Plus 555	AF3688AFP555-100UG
Alexa Fluor Plus 594	AF3688AFP594-100UG
Alexa Fluor Plus 647	AF3688AFP647-100UG
Alexa Fluor Plus 680	AF3688AFP680-100UG
Phycoerythrin	FAB3688P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3688P
Carrier Free	FAB3688G
Carrier Free	AF3688-SP

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MAB7498

LRIG1 Antibody (789211)

(1 Publication)

100 μg

MAB7498

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox">

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="LNCaP human prostate cancer cell line was stained with Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LRIG1 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

LRIG1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LRIG1 Monoclonal Antibody (Catalog # MAB7498) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7498U-100UG
Alexa Fluor 405	FAB7498V-100UG
Alexa Fluor 488	FAB7498G-100UG
Alexa Fluor 594	FAB7498T-100UG
Alexa Fluor 647	FAB7498R-100UG
Alexa Fluor 700	FAB7498N-100UG
Alexa Fluor 750	FAB7498S-100UG
Alexa Fluor Plus 405	FAB7498AFP405-100UG
Alexa Fluor Plus 488	FAB7498AFP488-100UG
Alexa Fluor Plus 555	FAB7498AFP555-100UG
Alexa Fluor Plus 594	FAB7498AFP594-100UG
Alexa Fluor Plus 647	FAB7498AFP647-100UG
Alexa Fluor Plus 680	FAB7498AFP680-100UG
Allophycocyanin	FAB7498A
Phycoerythrin	FAB7498P-025
Phycoerythrin	FAB7498P-100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB7498A
Carrier Free	MAB7498-SP
Carrier Free	FAB7498P-025
Carrier Free	FAB7498V-100UG
Carrier Free	FAB7498U-100UG
Carrier Free	FAB7498T-100UG
Carrier Free	FAB7498S-100UG
Carrier Free	FAB7498R-100UG
Carrier Free	FAB7498N-100UG
Carrier Free	FAB7498G-100UG
Carrier Free	FAB7498P-100

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AF7498

LRIG1 Antibody [Unconjugated]

(1 Publication)

100 μg

AF7498

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox">

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." alt="LNCaP human prostate cancer cell line was stained with Sheep Anti-Human LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7498, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

LRIG1 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7498) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7498U-100UG
Alexa Fluor 405	AF7498V-100UG
Alexa Fluor 488	FAB8140G
Alexa Fluor 532	AF7498X-100UG
Alexa Fluor 594	AF7498T-100UG
Alexa Fluor 647	AF7498R-100UG
Alexa Fluor 700	AF7498N-100UG
Alexa Fluor 750	AF7498S-100UG
Alexa Fluor Plus 405	AF7498AFP405-100UG
Alexa Fluor Plus 488	AF7498AFP488-100UG
Alexa Fluor Plus 555	AF7498AFP555-100UG
Alexa Fluor Plus 594	AF7498AFP594-100UG
Alexa Fluor Plus 647	AF7498AFP647-100UG
Alexa Fluor Plus 680	AF7498AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB8140G
Carrier Free	AF7498-SP

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MAB3688

LRIG1 Antibody (440710)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3688U-100UG
Alexa Fluor 405	FAB3688V-100UG
Alexa Fluor 532	FAB3688X-100UG
Alexa Fluor 594	FAB3688T-100UG
Alexa Fluor 647	FAB3688R-100UG
Alexa Fluor 700	FAB3688N-100UG
Alexa Fluor 750	FAB3688S-100UG
Alexa Fluor Plus 405	FAB3688AFP405-100UG
Alexa Fluor Plus 488	FAB3688AFP488-100UG
Alexa Fluor Plus 555	FAB3688AFP555-100UG
Alexa Fluor Plus 594	FAB3688AFP594-100UG
Alexa Fluor Plus 647	FAB3688AFP647-100UG
Alexa Fluor Plus 680	FAB3688AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3688-SP

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3688-LR

Recombinant Mouse LRIG1 Protein, CF

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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8504-LR

Recombinant Human LRIG1 Protein, CF

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-18504

LRIG1 Antibody - BSA Free

100 μg

NBP3-18504

Western Blot;LRIG1 Antibody Whole cell lysate (50 µg) from LNCaP, Hep-G2, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LRIG1 antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-18504AF350
Alexa Fluor 405	NBP3-18504AF405
Alexa Fluor 488	NBP3-18504AF488
Alexa Fluor 532	NBP3-18504AF532
Alexa Fluor 594	NBP3-18504AF594
Alexa Fluor 647	NBP3-18504AF647
Alexa Fluor 700	NBP3-18504AF700
Alexa Fluor 750	NBP3-18504AF750
Alexa Fluor Plus 405	NBP3-18504AFP405
Alexa Fluor Plus 488	NBP3-18504AFP488
Alexa Fluor Plus 555	NBP3-18504AFP555
Alexa Fluor Plus 594	NBP3-18504AFP594
Alexa Fluor Plus 647	NBP3-18504AFP647
Alexa Fluor Plus 680	NBP3-18504AFP680
Allophycocyanin	NBP3-18504APC
Biotin	NBP3-18504B
CoraFluor 1	NBP3-18504CL1
DyLight 350	NBP3-18504UV
DyLight 405	NBP3-18504V
DyLight 488	NBP3-18504G
DyLight 550	NBP3-18504R
DyLight 594	NBP3-18504DL594
DyLight 650	NBP3-18504C
DyLight 680	NBP3-18504FR
DyLight 755	NBP3-18504IR
FITC	NBP3-18504F
HRP	NBP3-18504H
Janelia Fluor 525	NBP3-18504JF525
Janelia Fluor 549	NBP3-18504JF549
Janelia Fluor 646	NBP3-18504JF646
Janelia Fluor 669	NBP3-18504JF669
PE	NBP3-18504PE
PerCP	NBP3-18504PCP
mFluor Violet 450 SE	NBP3-18504MFV450
mFluor Violet 500 SE	NBP3-18504MFV500
mFluor Violet 610 SE	NBP3-18504MFV610

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NBP2-30926

LRIG1 Antibody - BSA Free

0.1 ml

NBP2-30926

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-30926-25ul

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NBP2-30926PEP

LRIG1 Recombinant Protein Antigen

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF7498AFP594

LRIG1 Antibody [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Conjugate:

Alexa Fluor Plus 594

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FAB3688AFP555

LRIG1 Antibody (440710) [Alexa Fluor™ Plus 555]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor Plus 555

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AF3688AFP488

LRIG1 Antibody [Alexa Fluor™ Plus 488]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 488

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

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