HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human, mouse, and rat brain (cerebellum) tissue and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2504-SP | |||
HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2195-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89510-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87477-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2195-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98207-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89508-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79466-5Plates | |||
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-515-Immunocytochemistry-Immunofluorescence-NBP2-89510-img0001.jpg "Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of human CA14 in human liver with rabbit monoclonal antibody (1:200). Positive staining was localized to membrane of hepatocyte.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-515-Immunohistochemistry-Paraffin-NBP2-89510-img0002.jpg)
![Western Blot: Rabbit Monoclonal Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - 20ug/lane human melanoma cancer cell lysates (Lane #1 SKMEL3, Lane #2 M202), blotted with Carbonic Anhydrase XIV/CA14 antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89510_rabbit-monoclonal-carbonic-anhydrase-xiv-ca14-antibody-515-western-blot-712202411490..png)
![Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Analysis in control (vector only transfected HEK293T lysate) and CA14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-Western-Blot-NBP1-87477-img0006.jpg "Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Analysis in control (vector only transfected HEK293T lysate) and CA14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Staining of human cell line SK-MEL-30 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87477-img0005.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody [NBP1-87477] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-Immunohistochemistry-Paraffin-NBP1-87477-img0002.jpg)
![Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP2-98206] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-Western-Blot-NBP2-98206-img0002.jpg "Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP2-98206] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa")
![Western Blot: Carbonic Anhydrase XIV Overexpression Lysate (Adult Normal) [NBL1-08614] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carbonic Anhydrase XIV.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08614-img0002.jpg "Western Blot: Carbonic Anhydrase XIV Overexpression Lysate (Adult Normal) [NBL1-08614] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carbonic Anhydrase XIV.")
![Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP2-98207] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-Western-Blot-NBP2-98207-img0001.jpg "Western Blot: Carbonic Anhydrase XIV/CA14 Antibody [NBP2-98207] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).")
![Western Blot: Carbonic Anhydrase XIV/CA14 Antibody (004) [NBP2-89508] - Lane A: Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:34 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-004-Western-Blot-NBP2-89508-img0001.jpg "Western Blot: Carbonic Anhydrase XIV/CA14 Antibody (004) [NBP2-89508] - Lane A: Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:34 kDa")
![Sandwich ELISA: Carbonic Anhydrase XIV/CA14 Antibody Pair [HRP] [NBP2-79466] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XIV-CA14-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79466-img0001.jpg "Sandwich ELISA: Carbonic Anhydrase XIV/CA14 Antibody Pair [HRP] [NBP2-79466] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
