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![Western Blot: MKRN1 Antibody [NB100-55304] - Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Western-Blot-NB100-55304-img0007.jpg "Western Blot: MKRN1 Antibody [NB100-55304] - Detection of human and mouse MKRN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: MKRN1 Antibody [NB100-55304] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MKRN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MKRN1-Antibody-Immunohistochemistry-NB100-55304-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MKRN1 Antibody [NB100-55304] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at for WB at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Western-Blot-NB100-55304-img0003.jpg)
![Immunohistochemistry-Paraffin: MKRN1 Antibody [NB100-55304] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-MKRN1 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Immunohistochemistry-Paraffin-NB100-55304-img0001.jpg)
![Immunoprecipitation: MKRN1 Antibody [NB100-55304] - Detection of human MKRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MKRN1 antibody NB100-55304 (lot NB100-55304-2) used for IP at 6 ug per reaction. MKRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-55304-1). For blotting immunoprecipitated MKRN1,NB100-55304 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Immunoprecipitation-NB100-55304-img0006.jpg)
![Western Blot: MKRN1 Antibody [NBP2-27545] - Staining of Mouse fetal (A) and adult (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Western-Blot-NBP2-27545-img0002.jpg "Western Blot: MKRN1 Antibody [NBP2-27545] - Staining of Mouse fetal (A) and adult (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: MKRN1 Antibody [NBP2-27545] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with NBP2-27545 (0.3ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1:1000) in lane C. Mock-transfected HEK293 probed with NBP2-27545 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Western-Blot-NBP2-27545-img0001.jpg)
![Western Blot: MKRN1 Antibody (3F9) [NBP2-01179] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1.](http://images.novusbio.com/fullsize/MKRN1-Antibody-3F9-Western-Blot-NBP2-01179-img0001.jpg "Western Blot: MKRN1 Antibody (3F9) [NBP2-01179] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1.")
![Western Blot: MKRN1 Antibody [NBP1-55226] - Jurkat cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/MKRN1-Antibody-Western-Blot-NBP1-55226-img0003.jpg "Western Blot: MKRN1 Antibody [NBP1-55226] - Jurkat cell lysate, concentration 1.25ug/ml.")
![Western Blot: MKRN1 Overexpression Lysate (Adult Normal) [NBL1-13126] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MKRN1.](http://images.novusbio.com/fullsize/MKRN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13126-img0002.jpg "Western Blot: MKRN1 Overexpression Lysate (Adult Normal) [NBL1-13126] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MKRN1.")
![Sandwich ELISA: MKRN1 Antibody (4E9) [H00023608-M01] - Detection limit for recombinant GST tagged MKRN1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKRN1-Antibody-4E9-Sandwich-ELISA-H00023608-M01-img0001.jpg "Sandwich ELISA: MKRN1 Antibody (4E9) [H00023608-M01] - Detection limit for recombinant GST tagged MKRN1 is 0.1 ng/ml as a capture antibody.")