| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1762SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56475SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76688-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21674-50ul | |||
![Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0007.jpg "Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0006.jpg)
![Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0001.jpg)
![Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Western-Blot-NB100-1762-img0004.jpg)
![Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0002.jpg)
![Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Immunohistochemistry-Paraffin-NB100-1762-img0003.jpg)
![Simple Western: LSD1 Antibody (1B2E5) [NB100-1762] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Simple-Western-NB100-1762-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Knockdown of KDM1A protein was confirmed by western blotting of whole-cell lysates from DAOY and ONS-76 cells. beta-actin served as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252778/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LSD1-Antibody-1B2E5-Knockdown-Validated-NB100-1762-img0008.jpg)
![Western Blot: LSD1 Antibody [NB100-56475] - Analysis of LSD1 in the 1) absence and 2) presence of immunizing peptide in human brain lysate using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NB100-56475-img0002.jpg "Western Blot: LSD1 Antibody [NB100-56475] - Analysis of LSD1 in the 1) absence and 2) presence of immunizing peptide in human brain lysate using this antibody. 5 ug/ml.")
![Western Blot: LSD1 Antibody [NBP1-76688] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LSD1 NBP1-76688 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP1-76688-img0005.jpg "Western Blot: LSD1 Antibody [NBP1-76688] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LSD1 NBP1-76688 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: LSD1 Antibody [NBP1-76688] - P815 cell lysate at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP1-76688-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: LSD1 Antibody [NBP1-76688] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: LSD1 NBP1-76417 (0.2 ug/mL), LSD1 NBP1-76688 (1 ug/mL), beta-actin NBP1-76692 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP1-76688-img0004.jpg)
![Immunohistochemistry: LSD1 Antibody - BSA Free [NBP1-76688] - Validation of LSD1 in Human Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-LSD1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76688_rabbit-polyclonal-lsd1-antibody-271120242016928.jpg)
![Western Blot: LSD1 Antibody - BSA Free [NBP1-76688] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: LSD1 ( 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76688_rabbit-polyclonal-lsd1-antibody-2711202419592019.gif)
![Immunocytochemistry/ Immunofluorescence: LSD1 Antibody - BSA Free [NBP1-76688] - Validation of LSD1 in Human Small Intestine Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine cells labeling LSD1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76688_rabbit-polyclonal-lsd1-antibody-271120241951400.jpg)
![Western Blot: LSD1 Antibody [NBP3-18684] - Figure 5. Western blot analysis using the antibody directed against LSD1 Whole cell extracts (40 ug) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against LSD1 diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18684-img0002.jpg "Western Blot: LSD1 Antibody [NBP3-18684] - Figure 5. Western blot analysis using the antibody directed against LSD1 Whole cell extracts (40 ug) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against LSD1 diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: LSD1 Antibody [NBP3-18684] - Figure 6. Immunofluorescence using the antibody directed against LSD1 HeLa cells were stained with the antibody against LSD1 and with DAPI. Cells were fixed with 4% formaldehyde for 10 and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/LSD1 Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18684-img0006.jpg)
![Chromatin Immunoprecipitation Sequencing: LSD1 Antibody [NBP3-18684] - Figure 2. ChIP-seq results obtained with the antibody directed against LSD1 ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 ug of the antibody against LSD1 as described above. The IPd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturers instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.](http://images.novusbio.com/fullsize/LSD1-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP3-18684-img0005.jpg)
![Western Blot: LSD1 Antibody [NBP3-18684] - Figure 4. Western blot analysis using the antibody directed against LSD1 Western blot was performed using nuclear extracts from HeLa cells (40 ug) and the antibody against LSD1 diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18684-img0001.jpg)
![Chromatin Immunoprecipitation Sequencing: LSD1 Antibody [NBP3-18684] - Figure 1. ChIP results obtained with the antibody directed against LSD1 ChIP was performed with the antibody against LSD1 on sheared chromatin from 4,000,000 K562 cells using the](http://images.novusbio.com/fullsize/LSD1-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP3-18684-img0004.jpg)
![ELISA: LSD1 Antibody [NBP3-18684] - Figure 3. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against LSD1 in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.](http://images.novusbio.com/fullsize/LSD1-Antibody-ELISA-NBP3-18684-img0003.jpg)
![Western Blot: LSD1 Antibody [NB100-536] - Detection of human LSD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LSD1 antibody NB100-536 (lot NB100-536-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. LSD1 was also immunoprecipitated by a previous lot (lot NB100-536-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NB100-536-img0008.jpg "Western Blot: LSD1 Antibody [NB100-536] - Detection of human LSD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LSD1 antibody NB100-536 (lot NB100-536-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. LSD1 was also immunoprecipitated by a previous lot (lot NB100-536-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BHC110/LSD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/LSD1-Antibody-Immunohistochemistry-NB100-536-img0006.jpg)
![Western Blot: LSD1 Antibody [NB100-536] - Detection of Human BHC110/KIAA0601 by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: NB100-536 used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NB100-536-img0001.jpg)
![Western Blot: LSD1 Antibody [NBP3-18687] - Figure 3 Immunoprecipitation and Western blot analysis using the antibody directed against LSD1 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the antibody against human LSD1 , diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 ug of the antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18687-img0002.jpg "Western Blot: LSD1 Antibody [NBP3-18687] - Figure 3 Immunoprecipitation and Western blot analysis using the antibody directed against LSD1 293T cells were transiently transfected with an expression vector for HA-tagged LSD1 (lanes 1) or with an empty vector (lanes 2). Figure 3A: Whole cell extracts were analysed by Western blot using the antibody against human LSD1 , diluted 1:1,000. Figure 3B. Whole cell extracts were immunoprecipitated with 2 ug of the antibody against LSD1. Immunoprecipitates were analysed by Western blot as described above.")
![Western Blot: LSD1 Antibody [NBP3-18687] - Figure 2 Western blot analysis using the antibody directed against LSD1 Western blot was performed using respectively 150 ug (lane 1) or 50 ug (lane 2) nuclear extracts from HeLa cells and the antibody against LSD1 diluted 1:1,000. The location of the protein of interest is indicated on the left.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18687-img0001.jpg)
![ELISA: LSD1 Antibody [NBP3-18687] - Figure 1 Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human LSD1 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.](http://images.novusbio.com/fullsize/LSD1 Antibody-ELISA-NBP3-18687-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LSD1 Antibody (2E7) [H00023028-M04] - Analysis of monoclonal antibody to AOF2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/LSD1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-H00023028-M04-img0004.jpg "Immunocytochemistry/Immunofluorescence: LSD1 Antibody (2E7) [H00023028-M04] - Analysis of monoclonal antibody to AOF2 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: LSD1 Antibody (2E7) [H00023028-M04] - Analysis of monoclonal antibody to AOF2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml](http://images.novusbio.com/fullsize/LSD1-Antibody-2E7-Immunohistochemistry-Paraffin-H00023028-M04-img0005.jpg)
![ELISA: LSD1 Antibody (2E7) [H00023028-M04] - Detection limit for recombinant GST tagged AOF2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LSD1-Antibody-2E7-ELISA-H00023028-M04-img0007.jpg)
![Western Blot: LSD1 Antibody (SR1059) [NBP3-21674] - Western blot analysis using NBP3-21674 in Hela cell lysate](http://images.novusbio.com/fullsize/nbp3-21674_rabbit-lsd1-mab-sr1059-672023106218.jpg "Western Blot: LSD1 Antibody (SR1059) [NBP3-21674] - Western blot analysis using NBP3-21674 in Hela cell lysate")
![Immunohistochemistry: LSD1 Antibody (SR1059) [NBP3-21674] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21674.](http://images.novusbio.com/fullsize/nbp3-21674_rabbit-lsd1-mab-sr1059-5720231552182.jpg)
![Western Blot: LSD1 Antibody (1G7) [NBP3-15193] - Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate. All lanes: LSD1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 96 KDa. Observed band size: 110 KDa.](http://images.novusbio.com/fullsize/LSD1-Antibody-1G7-Western-Blot-NBP3-15193-img0003.jpg "Western Blot: LSD1 Antibody (1G7) [NBP3-15193] - Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate. All lanes: LSD1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 93, 96 KDa. Observed band size: 110 KDa.")
![Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193] - IHC image of LSD1 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/LSD1-Antibody-1G7-Immunohistochemistry-NBP3-15193-img0002.jpg)
![Immunohistochemistry: LSD1 Antibody (1G7) [NBP3-15193] - IHC image of LSD1 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/LSD1-Antibody-1G7-Immunohistochemistry-NBP3-15193-img0001.jpg)
![Western Blot: LSD1 Antibody [NBP3-18640] - Figure 1. Western blot analysis using the antibody directed against LSD1 Figure 1A: Western blot was performed with the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on purified recombinant LSD1 protein (50 and 25 ng, lane 1 and 2) and on nuclear extracts (50 ug) from HeLa and HEK293 cells (lane 3 and 4, respectively).](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18640-img0003.jpg "Western Blot: LSD1 Antibody [NBP3-18640] - Figure 1. Western blot analysis using the antibody directed against LSD1 Figure 1A: Western blot was performed with the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on purified recombinant LSD1 protein (50 and 25 ng, lane 1 and 2) and on nuclear extracts (50 ug) from HeLa and HEK293 cells (lane 3 and 4, respectively).")
![Western Blot: LSD1 Antibody [NBP3-18640] - Figure 2. Western blot analysis using the antibody directed against LSD1 Whole cell extracts (40 ug) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/LSD1 Antibody-Western Blot-NBP3-18640-img0002.jpg)
![Western Blot: LSD1 Antibody [NBP3-18640] - Figure 1. Western blot analysis using the antibody directed against LSD1 Figure 1B: Western blot was performed as described above using nuclear extracts from HeLa cells (HeLa NE, 20 ug). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18640-img0001.jpg)
![Western Blot: LSD1 Antibody (7R10X6) [NBP3-16688] - Western blot analysis of extracts of various cell lines, using LSD1 antibody (NBP3-16688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/LSD1-Antibody-7R10X6-Western-Blot-NBP3-16688-img0002.jpg "Western Blot: LSD1 Antibody (7R10X6) [NBP3-16688] - Western blot analysis of extracts of various cell lines, using LSD1 antibody (NBP3-16688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: LSD1 Antibody (7R10X6) [NBP3-16688] - Immunohistochemistry of paraffin-embedded human breast cancer using LSD1 Rabbit mAb (NBP3-16688) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LSD1-Antibody-7R10X6-Immunohistochemistry-Paraffin-NBP3-16688-img0001.jpg)
![Western Blot: LSD1 Antibody [NBP3-18670] - Figure 1. Western blot analysis using the antibody directed against LSD1 Western blot was performed on nuclear extracts from HeLa cells (20 ug) using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/LSD1-Antibody-Western-Blot-NBP3-18670-img0001.jpg "Western Blot: LSD1 Antibody [NBP3-18670] - Figure 1. Western blot analysis using the antibody directed against LSD1 Western blot was performed on nuclear extracts from HeLa cells (20 ug) using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![Western Blot: LSD1 Antibody [NBP3-18670] - Figure 2. Western blot analysis using the antibody directed against LSD1 Whole cell extracts (40 ug) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against LSD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/LSD1 Antibody-Western Blot-NBP3-18670-img0002.jpg)