235-F4) was serially diluted and captured by Mouse Anti-Human FGF‑4 Monoclonal Antibody (Catalog # MAB635) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human FGF‑4 (Catalog # 235-F4) was serially diluted and captured by Mouse Anti-Human FGF‑4 Monoclonal Antibody (Catalog # MAB635) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB635-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | Qk004-0500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5846-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76865-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83291-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37374-0.025ml | |||
![Recombinant HumanFGF-4, Animal-Free activity is determined using the firefly luciferase reporter assay (*) in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-4. Firefly luciferase activity is measured and normalized. EC<sub>50</sub> = 4.3 ng/mL (306.5 pM). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340](http://images.novusbio.com/fullsize/qk004_recombinant-human-fgf-4-animal-free-protein-882023163330.jpg "<b>Biological Strategies Validation. </b>Recombinant HumanFGF-4, Animal-Free activity is determined using the firefly luciferase reporter assay (*) in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-4. Firefly luciferase activity is measured and normalized. EC<sub>50</sub> = 4.3 ng/mL (306.5 pM). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340")
![Western Blot: FGF-4 Antibody [NBP1-76865] - 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/FGF-4-Antibody-Western-Blot-NBP1-76865-img0001.jpg "Western Blot: FGF-4 Antibody [NBP1-76865] - 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] - Immunofluorescence of FGF-4 in 3T3 cells with FGF-4 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76865_rabbit-polyclonal-fgf-4-antibody-2711202419313265.jpg)
![Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] - Immunocytochemistry of FGF-4 in 3T3 cells with FGF-4 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76865_rabbit-polyclonal-fgf-4-antibody-2711202419463488.jpg)
![Immunohistochemistry-Paraffin: FGF-4 Antibody [NBP1-83291] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts and leydig cells.](http://images.novusbio.com/fullsize/FGF-4-Antibody-Immunohistochemistry-Paraffin-NBP1-83291-img0002.jpg "Immunohistochemistry-Paraffin: FGF-4 Antibody [NBP1-83291] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts and leydig cells.")
![Western Blot: FGF-4 Antibody (3A12G9) [NBP2-37374] - Western blot analysis using FGF4 mAb against HEK293 (1) and FGF4 (AA: 62-123)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/FGF-4-Antibody-3A12G9-Western-Blot-NBP2-37374-img0003.jpg "Western Blot: FGF-4 Antibody (3A12G9) [NBP2-37374] - Western blot analysis using FGF4 mAb against HEK293 (1) and FGF4 (AA: 62-123)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: FGF-4 Antibody (3A12G9) [NBP2-37374] - Western blot analysis using FGF4 mAb against human FGF4 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/FGF-4-Antibody-3A12G9-Western-Blot-NBP2-37374-img0002.jpg)
![ELISA: FGF-4 Antibody (3A12G9) [NBP2-37374] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/FGF-4-Antibody-3A12G9-ELISA-NBP2-37374-img0001.jpg)
![Western Blot: FGF4 Overexpression Lysate (Adult Normal) [NBP2-08157] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF4.](http://images.novusbio.com/fullsize/FGF4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08157-img0001.jpg "Western Blot: FGF4 Overexpression Lysate (Adult Normal) [NBP2-08157] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FGF4.")
