HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5." alt="Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6727-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70552 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92028-0.02ml | |||
![Western Blot: DGKB Antibody (1F9) [NBP2-02733] Analysis of extracts (35ug) from 9 different cell lines by using anti-DGKB monoclonal antibody.](http://images.novusbio.com/fullsize/DGKB-Antibody-1F9-Western-Blot-NBP2-02733-img0002.jpg "Western Blot: DGKB Antibody (1F9) [NBP2-02733] Analysis of extracts (35ug) from 9 different cell lines by using anti-DGKB monoclonal antibody.")
![Western Blot: DGKB Antibody (1F9) [NBP2-02733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKB.](http://images.novusbio.com/fullsize/DGKB-Antibody-1F9-Western-Blot-NBP2-02733-img0001.jpg)
![Western Blot: DGK-beta Antibody [NBP2-92028] - Analysis of extracts of various cell lines, using DGK-beta at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/DGK-beta-Antibody-Western-Blot-NBP2-92028-img0001.jpg "Western Blot: DGK-beta Antibody [NBP2-92028] - Analysis of extracts of various cell lines, using DGK-beta at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: DGKB Overexpression Lysate (Adult Normal) [NBL1-09853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DGKB.](http://images.novusbio.com/fullsize/DGKB-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09853-img0002.jpg "Western Blot: DGKB Overexpression Lysate (Adult Normal) [NBL1-09853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DGKB.")
![Western Blot: DGKB Overexpression Lysate (Adult Normal) [NBP2-09799] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DGKB.](http://images.novusbio.com/fullsize/DGKB-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09799-img0001.jpg "Western Blot: DGKB Overexpression Lysate (Adult Normal) [NBP2-09799] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DGKB.")
