| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35492-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12770-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87414-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97723-50ul | |||
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202414112831.jpg "Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202414123897.jpg)
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202415121395.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP3-35492] - Western blot analysis of various lysates using Alanyl tRNA synthetase Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202415111883.jpg)
![Immunocytochemistry/ Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-35492] - Immunofluorescence analysis of 293T cells using Alanyl tRNA synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35492_rabbit-alanyl-trna-synthetase-pab-912202414213733.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A: NIH3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP3-12770-img0005.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A: NIH3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of mouse AARS in mouse kidney with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-12770-img0003.jpg)
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of mouse AARS in mouse stomach with rabbit polyclonal antibody at 1:10000 dilution.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-12770-img0002.jpg)
![Immunoprecipitation: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A:0.5 mg K562 Whole Cell Lysate0.5 uL anti-Mouse AARS rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-Mouse AARS rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 107 kDaObserved band size: 107 kDa](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP3-12770-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with NBP3-12770 antibody (1:5000) at 4? overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12770_rabbit-polyclonal-alanyl-trna-synthetase-antibody-235202511444418.png)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis using Anti-AARS antibody NBP1-87414 (A) shows similar pattern to independent antibody NBP2-58166 (B).](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-87414-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis using Anti-AARS antibody NBP1-87414 (A) shows similar pattern to independent antibody NBP2-58166 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-87414-img0009.jpg)
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP1-87414] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-87414-img0001.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-87414] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-87414-img0007.jpg)
![Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706] - Paraffin-embedded hepatoma; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP1-31706-img0003.jpg "Immunohistochemistry: Alanyl tRNA synthetase Antibody [NBP1-31706] - Paraffin-embedded hepatoma; antibody dilution 1:500.")
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-31706-img0002.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP1-31706] - Sample (50 ug of whole cell lysate) A: Mouse liver 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP1-31706-img0004.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166] - Analysis using Anti-AARS antibody NBP2-58166 (A) shows similar pattern to independent antibody NBP1-87414 (B).](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-58166-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166] - Analysis using Anti-AARS antibody NBP2-58166 (A) shows similar pattern to independent antibody NBP1-87414 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP2-58166] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58166-img0001.jpg)
![Western Blot: Alanyl tRNA synthetase Antibody [NBP2-15332] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-15332-img0002.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP2-15332] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP2-15332] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-15332-img0001.jpg)
![Western Blot:[NBP3-30487]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30487_rabbit-alanyl-trna-synthetase-pab-231202511585565.jpg "Western Blot:[NBP3-30487]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.")
![Western Blot:[NBP3-30081]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30081_rabbit-alanyl-trna-synthetase-pab-23120251252862.jpg "Western Blot:[NBP3-30081]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and , which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.")
![Western Blot: Alanyl tRNA synthetase Antibody [NBP2-97723] - Anti-Alanyl tRNA synthetase rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-97723-img0003.jpg "Western Blot: Alanyl tRNA synthetase Antibody [NBP2-97723] - Anti-Alanyl tRNA synthetase rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa")
![Immunoprecipitation: Alanyl tRNA synthetase Antibody [NBP2-97723] - Alanyl tRNA synthetase was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate. 4 ul anti-Alanyl tRNA synthetase rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Alanyl tRNA synthetase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-97723-img0004.jpg)
![SDS-Page: Recombinant Human Alanyl tRNA synthetase Protein [H00000016-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Alanyl-tRNA-synthetase-Protein-SDS-Page-H00000016-P01-img0001.jpg "SDS-Page: Recombinant Human Alanyl tRNA synthetase Protein [H00000016-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Alanyl tRNA synthetase Overexpression Lysate (Adult Normal) [NBL1-07165] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alanyl tRNA synthetase.](http://images.novusbio.com/fullsize/Alanyl-tRNA-synthetase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07165-img0002.jpg "Western Blot: Alanyl tRNA synthetase Overexpression Lysate (Adult Normal) [NBL1-07165] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alanyl tRNA synthetase.")