Products Related to Gene-ID: 1493

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CTLA4 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rabbit Anti-Human CTLA4, Monoclonal Antibody (Catalog # NBP3-20446 ) at 1:100 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF018). A specific band was detected for CTLA-4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CTLA-4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line transfected with with Human CTLA-4 and mouse lymph node tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse CTLA-4 Monoclonal Antibody (Catalog # MAB386) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CTLA-4 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB100) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (green line) as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). The ED₅₀ is typically 0.05-0.4 µg/mL." class="big_lightbox"> MAB100) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (green line) as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). The ED₅₀ is typically 0.05-0.4 µg/mL." class="big_thumb" />

F0111). Quadrants were set based on Normal Rabbit IgG Control Antibody (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrants were set based on Normal Rabbit IgG Control Antibody (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="NS0 mouse myeloma cell line transfected with (A) human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3252) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrants were set based on Normal Rabbit IgG Control Antibody (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 Human cell line transfected with (A) Human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CTLA-4 (Research Grade Ipilimumab) Monoclonal Antibody (Catalog # MAB9928) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

10133-B1) is immobilized at 0.5 µg/mL, Biotinylated Recombinant Human CTLA-4 Fc Chimera Avi-tag (Catalog # AVI7268) binds with an ED₅₀ of 0.4-2.4 ng/mL." class="big_lightbox"> 10133-B1) is immobilized at 0.5 µg/mL, Biotinylated Recombinant Human CTLA-4 Fc Chimera Avi-tag (Catalog # AVI7268) binds with an ED₅₀ of 0.4-2.4 ng/mL." alt="When Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 10133-B1) is immobilized at 0.5 µg/mL, Biotinylated Recombinant Human CTLA-4 Fc Chimera Avi-tag (Catalog # AVI7268) binds with an ED₅₀ of 0.4-2.4 ng/mL." class="big_thumb" />

10133-B1) was measured at a concentration range between 82 pM and 21 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.2511 nM." class="big_lightbox"> 10133-B1) was measured at a concentration range between 82 pM and 21 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.2511 nM." class="big_thumb" />

MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." alt="NS0 mouse myeloma cell line transfected with human CTLA-4 and eGFP was stained with (A) Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3253) or (B) Normal Rabbit IgG Control Antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

140-B1) binds with an ED₅₀ of 5-30 ng/mL." class="big_lightbox"> 140-B1) binds with an ED₅₀ of 5-30 ng/mL." alt="When Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera (Catalog # 9336-CT) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) binds with an ED₅₀ of 5-30 ng/mL." class="big_thumb" />

F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 ug/mL PHA for 2 days were stained with Mouse Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3251) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />