| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1253AF405 | |||
| Alexa Fluor 488 | NB21-1253AF488 | |||
| Alexa Fluor 647 | NB21-1253AF647 | |||
| Alexa Fluor 700 | NB21-1253AF700 | |||
| Biotin | NB21-1253B | |||
| DyLight 405 | NB21-1253V | |||
| DyLight 488 | NB21-1253G | |||
| DyLight 550 | NB21-1253R | |||
| DyLight 650 | NB21-1253C | |||
| HRP | NB21-1253H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1253SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1015AF405 | |||
| Alexa Fluor 488 | NB21-1015AF488 | |||
| Alexa Fluor 647 | NB21-1015AF647 | |||
| Alexa Fluor 700 | NB21-1015AF700 | |||
| Biotin | NB21-1015B | |||
| DyLight 405 | NB21-1015V | |||
| DyLight 488 | NB21-1015G | |||
| DyLight 550 | NB21-1015R | |||
| DyLight 650 | NB21-1015C | |||
| FITC | NB21-1015F | |||
| HRP | NB21-1015H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1015SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1012AF405 | |||
| Alexa Fluor 488 | NB21-1012AF488 | |||
| Alexa Fluor 647 | NB21-1012AF647 | |||
| Alexa Fluor 700 | NB21-1012AF700 | |||
| Biotin | NB21-1012B | |||
| DyLight 405 | NB21-1012V | |||
| DyLight 488 | NB21-1012G | |||
| DyLight 550 | NB21-1012R | |||
| DyLight 650 | NB21-1012C | |||
| FITC | NB21-1012F | |||
| HRP | NB21-1012H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1012SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1101AF405 | |||
| Alexa Fluor 488 | NB21-1101AF488 | |||
| Alexa Fluor 647 | NB21-1101AF647 | |||
| Alexa Fluor 700 | NB21-1101AF700 | |||
| Biotin | NB21-1101B | |||
| DyLight 405 | NB21-1101V | |||
| DyLight 488 | NB21-1101G | |||
| DyLight 550 | NB21-1101R | |||
| DyLight 650 | NB21-1101C | |||
| FITC | NB21-1101F | |||
| HRP | NB21-1101H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1101SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1053AF405 | |||
| Alexa Fluor 488 | NB21-1053AF488 | |||
| Alexa Fluor 647 | NB21-1053AF647 | |||
| Alexa Fluor 700 | NB21-1053AF700 | |||
| Biotin | NB21-1053B | |||
| DyLight 405 | NB21-1053V | |||
| DyLight 488 | NB21-1053G | |||
| DyLight 550 | NB21-1053R | |||
| DyLight 650 | NB21-1053C | |||
| FITC | NB21-1053F | |||
| HRP | NB21-1053H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1053SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-12179-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1271V | |||
| FITC | NB21-1271F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1271-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25995 | |||
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NB21-1253] - Analysis of H3K36me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NB21-1253-img0002.jpg "Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NB21-1253] - Analysis of H3K36me3 in picomoles of peptide.")
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NB21-1253] - Analysis of Histone H3K36me3 in C. elegans embryo lysate. Observed molecular weight is ~18 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Western-Blot-NB21-1253-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody - BSA Free [NB21-1253] - Detectionof H3K36me3 in NIH/3T3 histone preps. Observed molecular weight is 15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody---BSA-Free-Western-Blot-NB21-1253-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys4, p Thr3] Antibody [NB21-1015] - Analysis of Histone H3pT3/K4ac in the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--ac-Lys4]-Antibody-Dot-Blot-NB21-1015-img0005.jpg "Dot Blot: Histone H3 [ac Lys4, p Thr3] Antibody [NB21-1015] - Analysis of Histone H3pT3/K4ac in the peptides listed above in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys4, p Thr3] Antibody [NB21-1015] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac--Lys4--p-Thr3]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1015-img0006.jpg)
![Western Blot: Histone H3 [ac Lys4, p Thr3] Antibody [NB21-1015] - Analysis of Histone H3 pT3/K4ac in 1: HeLa histone prep. 2: NIH3T3 histone prep. 3: C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--ac-Lys4]-Antibody-Western-Blot-NB21-1015-img0001.jpg)
![Dot Blot: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Analysis of H3pT3/K4me1 using the peptides listed above in the 100, 10 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Monomethyl-Lys4]-Antibody-Dot-Blot-NB21-1012-img0003.jpg "Dot Blot: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Analysis of H3pT3/K4me1 using the peptides listed above in the 100, 10 and 1 picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Monomethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1012-img0008.jpg)
![Western Blot: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Analysis of Histone H3 pT3/K4me1 in: 1: HeLa histone prep. 2: NIH 3T3 histone prep. 3: C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1012-img0007.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NB21-1101-img0003.jpg "Dot Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 with the peptides stated above in picomoles of peptide.")
![Western Blot: Histone H3 [p Thr11] Antibody [NB21-1101] - Analysis of H3pT11 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NB21-1101-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr11] Antibody [NB21-1101] - Staining of H3pT11 in HeLa cells using FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1101-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - 2 ug of NB21-1053 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1053-img0008.jpg "Chromatin Immunoprecipitation: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - 2 ug of NB21-1053 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Western Blot: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - Analysis of H3K9me2/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9-p-Thr6]-Antibody-Western-Blot-NB21-1053-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Dimethyl Lys9] Antibody [NB21-1053] - Histone H3.2 K9me2 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Dimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1053-img0007.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NBP2-54617-img0003.jpg "Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - HeLa cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9/14ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunofluorescence-NBP2-54617-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - ChIP assays were performed using human HeLa cells, the antibody against H3K9/14ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-ac-Lys14-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54617-img0001.jpg)
![ELISA: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9/14ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-ELISA-NBP2-54617-img0002.jpg)
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To test the cross reactivity of the antibody against H3K9/14ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NBP2-54617-img0006.jpg)
![Protein Array: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Protein-Array-NBP2-54617-img0005.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-AH3-120-Western-Blot-NB120-12179-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa")
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-59199-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - ChIP assays were performed using HeLa cells, the antibody against H3K36me3 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59199-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me3 and the crude serum. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:19,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-59199-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K36me3 with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-59199-img0005.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Dot-Blot-NB21-1271-img0006.jpg "Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - WB analysis of Histone H3 K37me1 in 1. HeLa histone prep, 2. NIH 3T3 histone prep and 3. C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Western-Blot-NB21-1271-img0007.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Western-Blot-NB21-1271-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Immunohistochemistry staining of HepG2 cells using Clone RM166.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-24620241332474.png "Immunohistochemistry-Paraffin: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Immunohistochemistry staining of HepG2 cells using Clone RM166.")
![Western Blot: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM166 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-22620242311027.png)
![Multiplex Immunoassay: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-22620242311050.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM166 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-22620242311068.png)
![Sandwich ELISA: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Sandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using an anti-Histone H3 (1 ug/mL) as the capture antibody and biotinylated clone RM166 (2 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-2462024132632.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using Clone RM166 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-chromatin-immunoprecipitation-2462024225351..png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-24620241332436.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Immunohistochemistry staining of HepG2 cells using Clone RM161.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-226202422321216.png)
![Multiplex Immunoassay: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-226202422321241.png)
![Western Blot: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using RM161 at 0.25 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-2462024133247.png)
![Sandwich ELISA: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Sandwich ELISA against acetylated histone H3 at Lys 9 using Clone RM161 (1 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-24620241332451.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - ChIP performed on HeLa cells using Clone RM161 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-22620242311074.png)
![Western Blot: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61538 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Western-Blot-NBP2-61538-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using NBP2-61538 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61538 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Immunocytochemistry-Immunofluorescence-NBP2-61538-img0009.jpg)
![Immunohistochemistry: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Immunohistochemistry staining of HepG2 cells using NBP2-61538.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Immunohistochemistry-NBP2-61538-img0010.jpg)
![Chromatin Immunoprecipitation (ChIP): Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using 5 ug NBP2-61538. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Chromatin-Immunoprecipitation-ChIP-NBP2-61538-img0013.jpg)
![ELISA: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - Sandwich ELISA using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using NBP2-61547 at 1 ug/ml as the capture antibody and biotinylated anti-H3K18ac NBP2-61538 at 2ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-ELISA-NBP2-61538-img0004.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys18] Antibody (RM166) [NBP2-61538] - NBP2-61538 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys18-Antibody-RM166-Multiplex-Immunoassay-NBP2-61538-img0012.jpg)