| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25994 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56595SS | |||
| Azide and BSA Free | NBP2-33237 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1095SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1273V | |||
| FITC | NB21-1273F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1273SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1164AF405 | |||
| Alexa Fluor 488 | NB21-1164AF488 | |||
| Alexa Fluor 647 | NB21-1164AF647 | |||
| Alexa Fluor 700 | NB21-1164AF700 | |||
| Biotin | NB21-1164B | |||
| DyLight 405 | NB21-1164V | |||
| DyLight 488 | NB21-1164G | |||
| DyLight 550 | NB21-1164R | |||
| DyLight 650 | NB21-1164C | |||
| FITC | NB21-1164F | |||
| HRP | NB21-1164H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1164SS | |||
![Western Blot: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using NBP2-61536 at 0.25 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Western-Blot-NBP2-61536-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using NBP2-61536 at 0.25 ug/ml. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - ChIP performed on HeLa cells using Clone RM161 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61536_rabbit-monoclonal-histone-h3-ac-lys9-antibody-rm161-24620241332431.png)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61536 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Immunocytochemistry-Immunofluorescence-NBP2-61536-img0010.jpg)
![Immunohistochemistry: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Immunohistochemistry staining of HepG2 cells using NBP2-61536.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Immunohistochemistry-NBP2-61536-img0011.jpg)
![ELISA: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - Sandwich ELISA using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using NBP2-61547 at 1 ug/ml as the capture antibody and NBP2-61536 at 1 ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-ELISA-NBP2-61536-img0007.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys9] Antibody (RM161) [NBP2-61536] - NBP2-61536 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-RM161-Multiplex-Immunoassay-NBP2-61536-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K20me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~11 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-54620-img0005.jpg "Western Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K20me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~11 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - HeLa cells were stained with the antibody against H4K20me3 and with DAPI. Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Immunofluorescence-NBP2-54620-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - ChIP assays were performed using human K562 cells, the antibody against H4K20me3 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoters of the active genes EIF4A2 and GAPDH, used as negative controls, and for ZNF510 and the Sat2 satellite repeat region used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys20-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54620-img0001.jpg)
![ELISA: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K20me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,700.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-54620-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - To test the cross reactivity of the antibody against H4K20me3. A Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-54620-img0003.jpg)
![Protein Array: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Protein-Array-NBP2-54620-img0004.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Western-Blot-NBP2-54613-img0004.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - HeLa cells were stained with the antibody against H3K4me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Immunofluorescence-NBP2-54613-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - ChIP assays were performed using human K562 cells, the antibody against H3K4me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-3-Monomethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54613-img0008.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - To test the cross reactivity of the antibody against H3K4me1, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Dot-Blot-NBP2-54613-img0003.jpg)
![ELISA: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K4me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:10,300.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-ELISA-NBP2-54613-img0009.jpg)
![Protein Array: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Protein-Array-NBP2-54613-img0010.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-117C826-Western-Blot-NB100-56595-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa")
![Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.](http://images.novusbio.com/fullsize/antibody/nb21-1095_rabbit-histone-h3-p-ser10-ac-lys9-pab-western-blot-1132024114213.jpg "Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.")
![Dot Blot analysis of Histone H3 [p Ser10, ac Lys9] Antibody in the peptides listed in 1.0 and 0.5 picomoles of peptide.](http://images.novusbio.com/fullsize/antibody/nb21-1095_rabbit-histone-h3-p-ser10-ac-lys9-pab-dot-blot-1132024115254..jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NBP2-59187-img0003.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Panel B, C, D and E: staining of the cells with the H3K4me2 antibody after incubation of the antibody with 5 ng/uL blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K4, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K4me2 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. A: cells were immunofluorescently labeled with the H3K4me2 antibody (left) diluted 1:5,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - ChIP was performed with the antibody against H3K4me2 on sheared chromatin from 500,000 HeLaS3 cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration of the antibody of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0006.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K4me2 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:48,200.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-ELISA-NBP2-59187-img0001.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me2 with peptides containing other modifications of histone H3 and H4 and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59187-img0002.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NBP2-59180-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NBP2-59180] - ChIP assays were performed using human HeLa cells, the antibody against H3K9ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers speci c for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that acetylation of K9 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59180-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys9] Antibody [NBP2-59180] - HeLa cells were stained with the antibody against H3K9ac and with DAPI. Cells were mixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immuno uorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunofluorescence-NBP2-59180-img0003.jpg)
![ELISA: Histone H3 [ac Lys9] Antibody [NBP2-59180] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9ac in antigen coated wells. The antigen used was a peptide containing the histone modi cation of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,700.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-ELISA-NBP2-59180-img0005.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~18 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Western-Blot-NBP2-59198-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~18 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - HeLa cells were stained with the Diagenode antibody against H3K9me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9me3 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59198-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me3 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using the](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59198-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9me3 with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Dot-Blot-NBP2-59198-img0007.jpg)
![ELISA: Histone H3 [Trimethyl Lys9] Antibody [NBP2-59198] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-ELISA-NBP2-59198-img0009.jpg)
![Western Blot: Histone H3 [Trimethyl Lys37] Antibody [NB21-1273] - Analysis of Histone H3 K37me3 in (1) HeLa histone prep and (2) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys37]-Antibody-Western-Blot-NB21-1273-img0003.jpg "Western Blot: Histone H3 [Trimethyl Lys37] Antibody [NB21-1273] - Analysis of Histone H3 K37me3 in (1) HeLa histone prep and (2) C. elegans embryo lysate. Observed molecular weight is ~15 kDa.")
![Dot Blot: Histone H3 [Trimethyl Lys37] Antibody [NB21-1273] - Analysis of Histone H3 K37me3 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys37]-Antibody-Dot-Blot-NB21-1273-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys23] Antibody [NB21-1164] - Dot blot analysis of Histone H3 K23ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys23]-Antibody-Dot-Blot-NB21-1164-img0001.jpg "Dot Blot: Histone H3 [ac Lys23] Antibody [NB21-1164] - Dot blot analysis of Histone H3 K23ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys23] Antibody [NB21-1164] - Western blot analysis of Histone H3 K23ac in 1. NIH 3T3 histone prep lysate and 2. HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys23]-Antibody-Western-Blot-NB21-1164-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32431) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-26820241525101.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32431) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate Lane 4: COS-1 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 14 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32431) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-278202416331219.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (positive) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (negative) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Proteins loading: 100ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 15 seconds; ECL.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-28820241459255.jpg)
![Immunohistochemistry: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32431) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-29820241492625.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody (JE00-98) [NBP3-32431] - Immunocytochemistry analysis of HeLa cells labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32431) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32431_rabbit-histone-h3-dimethyl-lys4-mab-je00-98-2920241192379.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Western-Blot-NBP2-59200-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Mouse NIH3T3 cells were stained with the antibody against H3K9me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Immunofluorescence-NBP2-59200-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me2 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59200-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9me2 , crude serum and Flow through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys9]-Antibody-ELISA-NBP2-59200-img0002.jpg)