| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1251AF405 | |||
| Alexa Fluor 488 | NB21-1251AF488 | |||
| Alexa Fluor 647 | NB21-1251AF647 | |||
| Alexa Fluor 700 | NB21-1251AF700 | |||
| Biotin | NB21-1251B | |||
| DyLight 405 | NB21-1251V | |||
| DyLight 488 | NB21-1251G | |||
| DyLight 550 | NB21-1251R | |||
| DyLight 650 | NB21-1251C | |||
| FITC | NB21-1251F | |||
| HRP | NB21-1251H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1251SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1343AF405 | |||
| Alexa Fluor 488 | NB21-1343AF488 | |||
| Alexa Fluor 647 | NB21-1343AF647 | |||
| Alexa Fluor 700 | NB21-1343AF700 | |||
| Biotin | NB21-1343B | |||
| DyLight 405 | NB21-1343V | |||
| DyLight 488 | NB21-1343G | |||
| DyLight 550 | NB21-1343R | |||
| DyLight 650 | NB21-1343C | |||
| FITC | NB21-1343F | |||
| HRP | NB21-1343H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1343SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1037AF405 | |||
| Alexa Fluor 488 | NB21-1037AF488 | |||
| Alexa Fluor 647 | NB21-1037AF647 | |||
| Alexa Fluor 700 | NB21-1037AF700 | |||
| Biotin | NB21-1037B | |||
| DyLight 405 | NB21-1037V | |||
| DyLight 488 | NB21-1037G | |||
| DyLight 550 | NB21-1037R | |||
| DyLight 650 | NB21-1037C | |||
| FITC | NB21-1037F | |||
| HRP | NB21-1037H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1037SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1141AF405 | |||
| Alexa Fluor 488 | NB21-1141AF488 | |||
| Alexa Fluor 647 | NB21-1141AF647 | |||
| Alexa Fluor 700 | NB21-1141AF700 | |||
| Biotin | NB21-1141B | |||
| DyLight 405 | NB21-1141V | |||
| DyLight 488 | NB21-1141G | |||
| DyLight 550 | NB21-1141R | |||
| DyLight 650 | NB21-1141C | |||
| FITC | NB21-1141F | |||
| HRP | NB21-1141H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1141SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1013AF405 | |||
| Alexa Fluor 488 | NB21-1013AF488 | |||
| Alexa Fluor 647 | NB21-1013AF647 | |||
| Alexa Fluor 700 | NB21-1013AF700 | |||
| Biotin | NB21-1013B | |||
| DyLight 405 | NB21-1013V | |||
| DyLight 488 | NB21-1013G | |||
| DyLight 550 | NB21-1013R | |||
| DyLight 650 | NB21-1013C | |||
| FITC | NB21-1013F | |||
| HRP | NB21-1013H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1013SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-747-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1092AF405 | |||
| Alexa Fluor 488 | NB21-1092AF488 | |||
| Alexa Fluor 647 | NB21-1092AF647 | |||
| Alexa Fluor 700 | NB21-1092AF700 | |||
| Biotin | NB21-1092B | |||
| DyLight 405 | NB21-1092V | |||
| DyLight 488 | NB21-1092G | |||
| DyLight 550 | NB21-1092R | |||
| DyLight 650 | NB21-1092C | |||
| FITC | NB21-1092F | |||
| HRP | NB21-1092H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1092SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1012AF405 | |||
| Alexa Fluor 488 | NB21-1012AF488 | |||
| Alexa Fluor 647 | NB21-1012AF647 | |||
| Alexa Fluor 700 | NB21-1012AF700 | |||
| Biotin | NB21-1012B | |||
| DyLight 405 | NB21-1012V | |||
| DyLight 488 | NB21-1012G | |||
| DyLight 550 | NB21-1012R | |||
| DyLight 650 | NB21-1012C | |||
| FITC | NB21-1012F | |||
| HRP | NB21-1012H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1012SS | |||
![Dot Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 using the peptides stated in 10, 6, 3, 1, 0.6 and 0.3 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Dot-Blot-NB21-1251-img0008.jpg "Dot Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 using the peptides stated in 10, 6, 3, 1, 0.6 and 0.3 picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of Histone H3 K36me1 in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Western-Blot-NB21-1251-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Histone H3 K36me1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1251-img0013.jpg)
![Western Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Western-Blot-NB21-1251-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Antibody was tested in HeLa cells during telophase with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys56]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1343-img0009.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Antibody was tested in HeLa cells during telophase with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Dot Blot: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Analysis of H3K56me3 using the peptides stated above in 100, 10 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys56]-Antibody-Dot-Blot-NB21-1343-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Analysis of Histone H3K56me3 in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys56]-Antibody-Western-Blot-NB21-1343-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Antibody was tested in HeLa cells during prophase with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys56]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1343-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Aanalysis of H3K4me1/pt6 in C. elegans embryonic lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Antibody-Western-Blot-NB21-1037-img0004.jpg "Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Aanalysis of H3K4me1/pt6 in C. elegans embryonic lysate. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Staining of H3K4me1/pT6 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4--p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1037-img0007.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody [NB21-1037] - Detection of H3K4me1/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4-p-Thr6]-Antibody-Western-Blot-NB21-1037-img0001.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys4, p Thr6] Antibody - BSA Free [NB21-1037] - Analysis of H3K4me1/pT6 antibody in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4--p-Thr6]-Antibody---BSA-Free-Dot-Blot-NB21-1037-img0008.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of HIstone H3K18me1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Dot-Blot-NB21-1141-img0006.jpg "Dot Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of HIstone H3K18me1 in picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Detection of H3K18me1 in HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Western-Blot-NB21-1141-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1141-img0011.jpg)
![Western Blot: Histone H3 [Monomethyl Lys18] Antibody [NB21-1141] - Analysis of Histone H3K18me1 in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys18]-Antibody-Western-Blot-NB21-1141-img0007.jpg)
![Dot Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Dot Blot analysis of H3pT3/K4me2 using the peptides listed above in 100, 10 & 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Dot-Blot-NB21-1013-img0005.jpg "Dot Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Dot Blot analysis of H3pT3/K4me2 using the peptides listed above in 100, 10 & 1 picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Histone H3 pT3/K4me2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1013-img0012.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1013 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1013-img0010.jpg)
![Western Blot: Histone H3 [p Thr3, Dimethyl Lys4] Antibody [NB21-1013] - WB analysis of Histone H3 pT3/K4me2 in NIH-3T3 histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1013-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-54611-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - HeLa cells were stained with the antibody against H3K36me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K36me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Immunofluorescence-NBP2-54611-img0003.jpg)
![Chromatin Immunoprecipitation Sequencing: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - ChIP assays were performed using human K562 cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the coding region of the inactive MB gene and the Sat satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP2-54611-img0015.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with an Histone ChIP-seq kit, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the promoter and a region located 1 kb upstream of the promoter of the GAPDH gene, used as negative controls.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-54611-img0014.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - To test the cross reactivity of the antibody against H3K36me3, a Dot Blot analysis was performed with peptides containing other modifications or unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-54611-img0004.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K36me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:132,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-54611-img0012.jpg)
![Protein Array: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - The specificity of the antibody was demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. The figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark. The peptide array analysis shows a slight cross reaction with H4K20me3 that was not observed in dot blot.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Protein-Array-NBP2-54611-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3 diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-54614-img0013.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27me3 diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Human HeLa cells were stained with the antibody against H3K27me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54614-img0012.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me3, H3K9me3 and H3K36me3 modifications and the unmodified H3K27 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K27me3 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-54614-img0015.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive TSH2B and MYT1 genes, used as positive controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-54614-img0014.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3K27me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-ELISA-NBP2-54614-img0008.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-54614] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me3 with peptides containing other modifications of histone H3 and H4 and the unmodified H3K27 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. The figure shows a high specificity of the antibody for the modification of interest. Please note that the antibody also recognizes the modification if S28 is phosphorylated.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Dot-Blot-NBP2-54614-img0009.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [NBP3-15921] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-7W5T2-Western-Blot-NBP3-15921-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [NBP3-15921] - Western blot analysis of extracts of various cell lines, using Histone H3 antibody (NBP3-15921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [NBP3-15921] - Dot-blot analysis of all sorts of peptides using Histone H3 antibody (NBP3-15921) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-7W5T2-Dot-Blot-NBP3-15921-img0002.jpg)
![Immunohistochemistry: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Histone H3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-5220251725196.jpg)
![Immunohistochemistry: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Histone H3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517251922.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and H3K36me3 Rabbit mAb . The ChIP sequencing results indicate the enrichment pattern of H3K36me3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517202926.jpg)
![Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Immunoprecipitation analysis of 600 ug extracts of 293F cells using 5 ug Histone H3 antibody . Western blot was performed from the immunoprecipitate using Histone H3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517251960.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (7W5T2) [Histone H3] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15921_rabbit-histone-h3-trimethyl-lys-36-mab-7w5t2-52202517213568.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB100-747] - Human Prostate 5 ug/ml.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB100-747-img0001.jpg "Immunohistochemistry-Paraffin: Histone H3 Antibody [NB100-747] - Human Prostate 5 ug/ml.")
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - Western blot was performed on whole cell (50 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.2 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Western-Blot-NBP2-54612-img0016.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - Western blot was performed on whole cell (50 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.2 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K9me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - HeLa cells were stained with the Diagenode antibody against H3K9me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me3 antibody (left) diluted 1:250 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54612-img0017.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and for ZNF510 and the Sat2 satellite repeat, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-54612-img0012.jpg)
![Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - NIH 3T3 cells were stained with the antibody against H3K9me3, left or with the negative control recombinant antibody, right. The bottom panel shows counterstaining of the cells with DAPI. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Immunofluorescence-NBP2-54612-img0009.jpg)
![ELISA: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:42,700.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-ELISA-NBP2-54612-img0013.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - To test the cross reactivity of the antibody against H3K9me3, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Dot-Blot-NBP2-54612-img0014.jpg)
![Protein Array: Histone H3 [Trimethyl Lys9] Antibody [NBP2-54612] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:5,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys9]-Antibody-Protein-Array-NBP2-54612-img0015.jpg)
![Western Blot: Histone H3 [p Thr11, p Ser10] Antibody [NB21-1092] - Analysis of h3pS10/pT11 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10-p-Thr11]-Antibody-Western-Blot-NB21-1092-img0003.jpg "Western Blot: Histone H3 [p Thr11, p Ser10] Antibody [NB21-1092] - Analysis of h3pS10/pT11 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr11, p Ser10] Antibody [NB21-1092] - Staining of H3pS10/pT11 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11--p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1092-img0006.jpg)
![Western Blot: Histone H3 [p Thr11, p Ser10] Antibody [NB21-1092] - Detection of H3pS10/pT11 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10-p-Thr11]-Antibody-Western-Blot-NB21-1092-img0001.jpg)
![Dot Blot: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Analysis of H3pT3/K4me1 using the peptides listed above in the 100, 10 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Monomethyl-Lys4]-Antibody-Dot-Blot-NB21-1012-img0003.jpg "Dot Blot: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Analysis of H3pT3/K4me1 using the peptides listed above in the 100, 10 and 1 picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Monomethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1012-img0008.jpg)
![Western Blot: Histone H3 [p Thr3, Monomethyl Lys4] Antibody [NB21-1012] - Analysis of Histone H3 pT3/K4me1 in: 1: HeLa histone prep. 2: NIH 3T3 histone prep. 3: C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3--Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1012-img0007.jpg)