FAB3832P) and either (A) Mouse Anti-Human IL-22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG
2BFlow Cytometry Isotype Control (Catalog #
MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_lightbox">
FAB3832P) and either (A) Mouse Anti-Human IL-22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG
2BFlow Cytometry Isotype Control (Catalog #
MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog #
FAB3832P) and either (A) Mouse Anti-Human IL-22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG
2BFlow Cytometry Isotype Control (Catalog #
MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_thumb" />
FAB3832P) and either (A) Mouse Anti-Human IL-22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human IL-22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />
782-IL) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑22BP Fc Chimera Avi-tag (Catalog # AVI1087) binds with an ED50 of 50.0-400 ng/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant Human IL-22 Protein (782-IL) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑22BP Fc Chimera Avi-tag (Catalog # AVI1087) binds with an ED50 of 50.0-400 ng/mL." class="big_thumb" />
![Western Blot: IL-22BP/IL22 RA2 Antibody [NBP1-85455] - Analysis in control (vector only transfected HEK293T lysate) and IL22RA2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/IL-22BP-IL22-RA2-Antibody-Western-Blot-NBP1-85455-img0005.jpg "Western Blot: IL-22BP/IL22 RA2 Antibody [NBP1-85455] - Analysis in control (vector only transfected HEK293T lysate) and IL22RA2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![N/A IL-22BP/IL22 RA2 [Biotin]](http://images.novusbio.com/fullsize/elisa/IL22BP_DY108705_ELISA_2568.jpg "N/A IL-22BP/IL22 RA2 [Biotin]")
![Western Blot: IL-22BP/IL22 RA2 Antibody [NBP1-32246] - Sample(30 ug of whole cell lysate) A:HeLa S3 12% SDS PAGE, antibody diluted at 1:200.](http://images.novusbio.com/fullsize/IL-22BP-IL22-RA2-Antibody-Western-Blot-NBP1-32246-img0005.jpg "Western Blot: IL-22BP/IL22 RA2 Antibody [NBP1-32246] - Sample(30 ug of whole cell lysate) A:HeLa S3 12% SDS PAGE, antibody diluted at 1:200.")
![Immunohistochemistry-Paraffin: IL-22BP/IL22 RA2 Antibody [NBP1-32246] - Paraffin-embedded PC14 xenograft; antibody dilution 1:250.](http://images.novusbio.com/fullsize/IL-22BP-IL22-RA2-Antibody-Immunohistochemistry-Paraffin-NBP1-32246-img0004.jpg)
