HAF016). A specific band was detected for IQGAP2 at approximately 185 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP2 at approximately 185 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5068-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37885-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04682-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37884-25ul | |||
![Immunocytochemistry/Immunofluorescence: IQGAP2 Antibody [NBP2-37885] - Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane & vesicles.](http://images.novusbio.com/fullsize/IQGAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37885-img0004.jpg "Immunocytochemistry/Immunofluorescence: IQGAP2 Antibody [NBP2-37885] - Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane & vesicles.")
![Western Blot: IQGAP2 Antibody [NBP3-04682] - Analysis of extracts of various cell lines, using IQGAP2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/IQGAP2-Antibody-Western-Blot-NBP3-04682-img0001.jpg "Western Blot: IQGAP2 Antibody [NBP3-04682] - Analysis of extracts of various cell lines, using IQGAP2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunohistochemistry-Paraffin: IQGAP2 Antibody [NBP3-04682] - Immunohistochemistry of paraffin-embedded Rat kidney using IQGAP2 Rabbit pAb (NBP3-04682) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IQGAP2-Antibody-Immunohistochemistry-Paraffin-NBP3-04682-img0003.jpg)
![Immunohistochemistry-Paraffin: IQGAP2 Antibody [NBP3-04682] - Immunohistochemistry of paraffin-embedded Human breast cancer using IQGAP2 Rabbit pAb (NBP3-04682) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IQGAP2-Antibody-Immunohistochemistry-Paraffin-NBP3-04682-img0002.jpg)
![Immunohistochemistry: IQGAP2 Antibody (JE64-93) [NBP3-32481] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-IQGAP2 antibody (NBP3-32481) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32481) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32481_rabbit-iqgap2-mab-je64-93-29820241492676.jpg "Immunohistochemistry: IQGAP2 Antibody (JE64-93) [NBP3-32481] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-IQGAP2 antibody (NBP3-32481) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32481) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis of human placenta tissue lysates with NBP3-32481 at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32481 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32481_-western-blot-639204597794161828.jpg)
![Western blot analysis on different lysates with NBP3-32481 at 1/1,000 dilution. Lane 1: Human liver tissue lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32481 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32481_-western-blot-639204598087697805.jpg)
![Western Blot: IQGAP2 Antibody (JE64-54) [NBP3-32480] - Western blot analysis of IQGAP2 on human placenta tissue lysates with Rabbit anti-IQGAP2 antibody (NBP3-32480) at 1/500 dilution.<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 181 kDa<br/> Observed band size: 181 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32480) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32480_rabbit-iqgap2-mab-je64-54-27820241521249.jpg "Western Blot: IQGAP2 Antibody (JE64-54) [NBP3-32480] - Western blot analysis of IQGAP2 on human placenta tissue lysates with Rabbit anti-IQGAP2 antibody (NBP3-32480) at 1/500 dilution.<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 181 kDa<br/> Observed band size: 181 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32480) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IQGAP2 Antibody (JE64-54) [NBP3-32480] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-IQGAP2 antibody (NBP3-32480) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32480) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32480_rabbit-iqgap2-mab-je64-54-29820241420936.jpg)
![Western Blot: IQGAP2 Antibody (S02-1I7) [NBP3-19668] - Western blot detection of IQGAP2 in Ramos lysates using NBP3-19668. Predicted band size: 181kDa. Observed band size: 181kDa.](http://images.novusbio.com/fullsize/IQGAP2-Antibody-S02-1I7-Western-Blot-NBP3-19668-img0001.jpg "Western Blot: IQGAP2 Antibody (S02-1I7) [NBP3-19668] - Western blot detection of IQGAP2 in Ramos lysates using NBP3-19668. Predicted band size: 181kDa. Observed band size: 181kDa.")
![Western Blot: IQGAP2 Antibody (1B10) [H00010788-M01] - IQGAP2 monoclonal antibody (M01), clone 1B10 Analysis of IQGAP2 expression in HeLa.](http://images.novusbio.com/fullsize/IQGAP2-Antibody-1B10-Western-Blot-H00010788-M01-img0004.jpg "Western Blot: IQGAP2 Antibody (1B10) [H00010788-M01] - IQGAP2 monoclonal antibody (M01), clone 1B10 Analysis of IQGAP2 expression in HeLa.")
![Sandwich ELISA: IQGAP2 Antibody (1B10) [H00010788-M01] - Detection limit for recombinant GST tagged IQGAP2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IQGAP2-Antibody-1B10-Sandwich-ELISA-H00010788-M01-img0003.jpg)
