| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5120-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77160-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48503-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57238-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98547-50ul | |||
![Western Blot: PIAS3 Antibody (JE30-36) [NBP3-32820] - Western blot analysis of PIAS3 on different lysates with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: HL-60 cell lysate Lane 3: Rat testis tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32820) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32820_rabbit-pias3-mab-je30-36-288202412461661.jpg "Western Blot: PIAS3 Antibody (JE30-36) [NBP3-32820] - Western blot analysis of PIAS3 on different lysates with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: HL-60 cell lysate Lane 3: Rat testis tissue lysate (20 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 68 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32820) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PIAS3 Antibody (JE30-36) [NBP3-32820] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32820) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32820_rabbit-pias3-mab-je30-36-298202414262620.jpg)
![Immunocytochemistry/ Immunofluorescence: PIAS3 Antibody (JE30-36) [NBP3-32820] - Immunocytochemistry analysis of NCI-H441 cells labeling PIAS3 with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PIAS3 antibody (NBP3-32820) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32820_rabbit-pias3-mab-je30-36-2920241192381.jpg)
![Western Blot: PIAS3 Antibody [NBP1-77160] - K562 cell lysate with PIAS3 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Western-Blot-NBP1-77160-img0001.jpg "Western Blot: PIAS3 Antibody [NBP1-77160] - K562 cell lysate with PIAS3 antibody at 1 ug/mL.")
![Western Blot: PIAS3 Antibody [NBP1-77160] - Western for PIAS3 in THP1 macrophage cells, Image from verified customer review.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Western-Blot-NBP1-77160-img0003.jpg)
![Immunohistochemistry: PIAS3 Antibody - BSA Free [NBP1-77160] - Immunohistochemistry of PIAS3 in human breast carcinoma tissue with PIAS3 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77160_rabbit-polyclonal-pias3-antibody-2711202420175189.jpg)
![Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12. Lane 1: PIAS3 transfected lysatE (67 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIAS3-Antibody-4F12-Western-Blot-H00010401-M03-img0003.jpg "Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12. Lane 1: PIAS3 transfected lysatE (67 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Western blot analysis of PIAS3 over-expressed 293 cell line, cotransfected with PIAS3 Validated Chimera RNAi or non-transfected control. Blot probed with H00010401-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/PIAS3-Antibody-4F12-Western-Blot-H00010401-M03-img0002.jpg)
![Immunohistochemistry: PIAS3 Antibody [NBP2-48503] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Immunohistochemistry-NBP2-48503-img0001.jpg "Immunohistochemistry: PIAS3 Antibody [NBP2-48503] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.")
![Immunocytochemistry/Immunofluorescence: PIAS3 Antibody [NBP2-57238] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57238-img0001.jpg "Immunocytochemistry/Immunofluorescence: PIAS3 Antibody [NBP2-57238] - Staining of human cell line MCF7 shows localization to nucleoplasm.")
![Western Blot: PIAS3 Overexpression Lysate (Adult Normal) [NBL1-14387] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIAS3.](http://images.novusbio.com/fullsize/PIAS3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14387-img0002.jpg "Western Blot: PIAS3 Overexpression Lysate (Adult Normal) [NBL1-14387] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIAS3.")
![Immunohistochemistry-Paraffin: PIAS3 Antibody [NBP2-98547] - Immunochemical staining of human PIAS3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PIAS3-Antibody-Immunohistochemistry-Paraffin-NBP2-98547-img0001.jpg "Immunohistochemistry-Paraffin: PIAS3 Antibody [NBP2-98547] - Immunochemical staining of human PIAS3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")